The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer
Introduction Copy number variation is a significant characteristic of colorectal cancer progression. RBFOX1 (A2BP1) is the gene with the highest frequency of copy number loss in colorectal cancer, but current research related to it and colorectal cancer is relatively scarce. Methods Data from TCGA a...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2025-05-01
|
| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/15330338251333695 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849732792783470592 |
|---|---|
| author | Jian Li MM Youheng Wang MM Jian Xu MS Min Niu BS Songlin Wan MD Yi Liu BS Zhao Ding MD Guangchun Li MD Qun Qian MD Daojiang Li MD |
| author_facet | Jian Li MM Youheng Wang MM Jian Xu MS Min Niu BS Songlin Wan MD Yi Liu BS Zhao Ding MD Guangchun Li MD Qun Qian MD Daojiang Li MD |
| author_sort | Jian Li MM |
| collection | DOAJ |
| description | Introduction Copy number variation is a significant characteristic of colorectal cancer progression. RBFOX1 (A2BP1) is the gene with the highest frequency of copy number loss in colorectal cancer, but current research related to it and colorectal cancer is relatively scarce. Methods Data from TCGA and other sources were used to analyze the copy number variation and mRNA expression levels of RBFOX1, as well as their correlation with clinical pathological data. Immunohistochemistry and immunofluorescence experiments were used to analyze the expression of RBFOX1 protein in colorectal cancer cells and tissues. Results RBFOX1 has a high frequency (22.4%) of copy number loss and diverse copy number variations in colorectal cancer tissues. High-level RBFOX1 deletion is prone to occur in the right-sided colon and tissues with high microsatellite instability. The copy number variation of RBFOX1 and mRNA expression are not correlated. In tumor tissues, RBFOX1 mRNA shows a characteristic of reduced expression, which is significantly related to BRAF mutation (P = 4.7e-05, P = 0.03). Low expression of RBFOX1 is prone to occur in the right-sided colon and tissues with high microsatellite instability. The protein encoded by RBFOX1 is expressed in normal intestinal tissues, but shows a characteristic of absence in some colorectal cancer tissues. Conclusion In the right-sided colon and tissues with high microsatellite instability, RBFOX1 shows copy number loss and low mRNA expression. This characteristic is closely related to BRAF gene mutation, and the protein of RBFOX1 is absent in some colorectal cancer tissues. |
| format | Article |
| id | doaj-art-c78a04d53cd94ec28464bcf6252907ea |
| institution | DOAJ |
| issn | 1533-0338 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj-art-c78a04d53cd94ec28464bcf6252907ea2025-08-20T03:08:13ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382025-05-012410.1177/15330338251333695The Expression Characteristics of the RBFOX1 Gene in Colorectal CancerJian Li MM0Youheng Wang MM1Jian Xu MS2Min Niu BS3 Songlin Wan MD4Yi Liu BS5Zhao Ding MD6Guangchun Li MD7Qun Qian MD8Daojiang Li MD9 JingMen People's Hospital, Jingchu University of Technology Affiliated JingMen People's Hospital Quality Control Center of Colorectal and Anal Surgery of Health Commission of Hubei Province, Wuhan, Hubei Province, China Department of Pathology of Zhongnan Hospital of Wuhan University, Wuhan, Hubei Province, China Operating Room of Zhongnan Hospital of Wuhan University, Wuhan, Hubei Province, China Quality Control Center of Colorectal and Anal Surgery of Health Commission of Hubei Province, Wuhan, Hubei Province, China Quality Control Center of Colorectal and Anal Surgery of Health Commission of Hubei Province, Wuhan, Hubei Province, China Quality Control Center of Colorectal and Anal Surgery of Health Commission of Hubei Province, Wuhan, Hubei Province, China Quality Control Center of Colorectal and Anal Surgery of Health Commission of Hubei Province, Wuhan, Hubei Province, China Quality Control Center of Colorectal and Anal Surgery of Health Commission of Hubei Province, Wuhan, Hubei Province, China Quality Control Center of Colorectal and Anal Surgery of Health Commission of Hubei Province, Wuhan, Hubei Province, ChinaIntroduction Copy number variation is a significant characteristic of colorectal cancer progression. RBFOX1 (A2BP1) is the gene with the highest frequency of copy number loss in colorectal cancer, but current research related to it and colorectal cancer is relatively scarce. Methods Data from TCGA and other sources were used to analyze the copy number variation and mRNA expression levels of RBFOX1, as well as their correlation with clinical pathological data. Immunohistochemistry and immunofluorescence experiments were used to analyze the expression of RBFOX1 protein in colorectal cancer cells and tissues. Results RBFOX1 has a high frequency (22.4%) of copy number loss and diverse copy number variations in colorectal cancer tissues. High-level RBFOX1 deletion is prone to occur in the right-sided colon and tissues with high microsatellite instability. The copy number variation of RBFOX1 and mRNA expression are not correlated. In tumor tissues, RBFOX1 mRNA shows a characteristic of reduced expression, which is significantly related to BRAF mutation (P = 4.7e-05, P = 0.03). Low expression of RBFOX1 is prone to occur in the right-sided colon and tissues with high microsatellite instability. The protein encoded by RBFOX1 is expressed in normal intestinal tissues, but shows a characteristic of absence in some colorectal cancer tissues. Conclusion In the right-sided colon and tissues with high microsatellite instability, RBFOX1 shows copy number loss and low mRNA expression. This characteristic is closely related to BRAF gene mutation, and the protein of RBFOX1 is absent in some colorectal cancer tissues.https://doi.org/10.1177/15330338251333695 |
| spellingShingle | Jian Li MM Youheng Wang MM Jian Xu MS Min Niu BS Songlin Wan MD Yi Liu BS Zhao Ding MD Guangchun Li MD Qun Qian MD Daojiang Li MD The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer Technology in Cancer Research & Treatment |
| title | The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer |
| title_full | The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer |
| title_fullStr | The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer |
| title_full_unstemmed | The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer |
| title_short | The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer |
| title_sort | expression characteristics of the rbfox1 gene in colorectal cancer |
| url | https://doi.org/10.1177/15330338251333695 |
| work_keys_str_mv | AT jianlimm theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT youhengwangmm theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT jianxums theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT minniubs theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT songlinwanmd theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT yiliubs theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT zhaodingmd theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT guangchunlimd theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT qunqianmd theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT daojianglimd theexpressioncharacteristicsoftherbfox1geneincolorectalcancer AT jianlimm expressioncharacteristicsoftherbfox1geneincolorectalcancer AT youhengwangmm expressioncharacteristicsoftherbfox1geneincolorectalcancer AT jianxums expressioncharacteristicsoftherbfox1geneincolorectalcancer AT minniubs expressioncharacteristicsoftherbfox1geneincolorectalcancer AT songlinwanmd expressioncharacteristicsoftherbfox1geneincolorectalcancer AT yiliubs expressioncharacteristicsoftherbfox1geneincolorectalcancer AT zhaodingmd expressioncharacteristicsoftherbfox1geneincolorectalcancer AT guangchunlimd expressioncharacteristicsoftherbfox1geneincolorectalcancer AT qunqianmd expressioncharacteristicsoftherbfox1geneincolorectalcancer AT daojianglimd expressioncharacteristicsoftherbfox1geneincolorectalcancer |