Dietary Supplementation of <i>Bacillus subtilis</i> as Probiotic Influenced the Growth Performance, Hematological Parameters, Immune Function, Antioxidant Status, and Digestive Enzyme Activity of Nile Tilapia Fingerlings (<i>Oreochromis niloticus</i>)

Dietary Supplementation of <i>Bacillus subtilis</i> as Probiotic Influenced the Growth Performance, Hematological Parameters, Immune Function, Antioxidant Status, and Digestive Enzyme Activity of Nile Tilapia Fingerlings (<i>Oreochromis niloticus</i>)

This study evaluated the impact of <i>Bacillus subtilis</i> supplementation on growth, antioxidant status, digestive enzyme activities, hematological parameters, blood biochemistry, and immune responses in Nile tilapia (<i>Oreochromis niloticus</i>) fingerlings. A total of 18...

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Bibliographic Details
Main Authors: Hairui Yu, Sadia Nazir, Farah Ijaz, Muhammad Umer Zahid, Maida Mushtaq, Muhammad Khan, Abdur Rahman, Muhammad Aziz Ur Rahman
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Animals
Subjects:
<i>B. subtilis</i>
growth promotor
dietary supplementation
growth
health
immunity
Online Access:https://www.mdpi.com/2076-2615/15/9/1256
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Summary:This study evaluated the impact of <i>Bacillus subtilis</i> supplementation on growth, antioxidant status, digestive enzyme activities, hematological parameters, blood biochemistry, and immune responses in Nile tilapia (<i>Oreochromis niloticus</i>) fingerlings. A total of 180 fingerlings (mean weight: 5 ± 0.5 g) were randomly assigned to four groups: a control group and three probiotic-supplemented groups with 10<sup>6</sup> (S-1), 10<sup>8</sup> (S-2), and 10<sup>10</sup> (S-3) CFU g<sup>−1</sup> of <i>B. subtilis</i>, following a completely randomized design. Fish were fed twice daily for eight weeks. Results showed that the 10<sup>10</sup> CFU g<sup>−1</sup> group exhibited significantly higher weight gain (43 g), specific growth rate (2.5%/day), and better feed conversion ratio (0.7) compared to the control (30 g, 2.2%/day, and 1.4, respectively). Hematological parameters (WBC, RBC, hemoglobin) and immune responses (phagocytic activity, lysozyme) were significantly improved. Blood biochemistry showed decreased ALT/AST levels and increased ALP activity. Digestive enzymes (protease, lipase, amylase) and antioxidant activity (GPx, CAT, SOD) were enhanced, with reduced MDA levels. No significant differences were observed in survival rates or stress responses. These findings suggest that <i>B. subtilis</i> supplementation at 10<sup>10</sup> CFU g<sup>−1</sup> improves growth, immune function, and antioxidant status, making it a promising probiotic for Nile tilapia.
ISSN:2076-2615

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