The synergy between Sri Lankan bee honey and silver nanoparticles on pathogenic bacteria
The study was to assess the antimicrobial activity of A1-silver-nanoparticles (A1AgNPs) in combination with Sri Lankan bee-honey (SLBH) on medically important Gram-negative and positive bacteria. The A1AgNPs were synthesized following the reduction of silver nitrates by sodium citrate. Using SLBH 20...
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Elsevier
2025-06-01
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| Series: | The Microbe |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194625001414 |
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| author | Kithsiri Senanayake Wikum Widuranga Kumbukgolla Jayaweera Arachchige Asela Sampath Jayaweera |
| author_facet | Kithsiri Senanayake Wikum Widuranga Kumbukgolla Jayaweera Arachchige Asela Sampath Jayaweera |
| author_sort | Kithsiri Senanayake |
| collection | DOAJ |
| description | The study was to assess the antimicrobial activity of A1-silver-nanoparticles (A1AgNPs) in combination with Sri Lankan bee-honey (SLBH) on medically important Gram-negative and positive bacteria. The A1AgNPs were synthesized following the reduction of silver nitrates by sodium citrate. Using SLBH 20 % (v⁄v) stock solution, a dilution series from 1 % (v⁄v) intervals between 0 % and 10 % were made. The agar plates that contain A1AgNPs and SLBH were separately inoculated using bacterial suspension. Each organism's fractional inhibitory concentration index (ΣFIC) was calculated to assess the synergy. The minimum inhibitory concentration (MIC) of A1AgNPs for E. coli (6.7 ± 0.48), Shigella dysenteriae (6.66 ± 0.51), P. vulgaris (7.16 ± 0.4), P. aeruginosa (7.1 ± 0.33) and Salmonella Typhimurium (7.8 ± 0.83) were given in v/v%. Once the addition of equal volumes of A1AgNPs and SLBH, the spectrum of activity of the A1AgNPs improved from Gram-negative spectrum to Gram-positive, including MRSA (MIC 4.31 ± 0.47). Also, MIC values were further reduced, indicating a synergistic activity between the A1AgNPs and SLBH and E. coli, S. dysenteriae, P. vulgaris, P. aeruginosa, and Salmonella Typhimurium ΣFIC was 0.48, 0.48, 0.48, 0.48, and 0.44, respectively. In the future, SLBH can be used as a base for synthetic A1AgNPs to treat pathogenic bacteria. |
| format | Article |
| id | doaj-art-c77826734ee840b4bbc97dc395f5f0dd |
| institution | DOAJ |
| issn | 2950-1946 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Microbe |
| spelling | doaj-art-c77826734ee840b4bbc97dc395f5f0dd2025-08-20T02:57:33ZengElsevierThe Microbe2950-19462025-06-01710037310.1016/j.microb.2025.100373The synergy between Sri Lankan bee honey and silver nanoparticles on pathogenic bacteriaKithsiri Senanayake0Wikum Widuranga Kumbukgolla1Jayaweera Arachchige Asela Sampath Jayaweera2Department of Surgery, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura 50008, Sri LankaDepartment of Biochemistry, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura 50008, Sri LankaDepartment of Microbiology, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura 50008, Sri Lanka; Corresponding author.The study was to assess the antimicrobial activity of A1-silver-nanoparticles (A1AgNPs) in combination with Sri Lankan bee-honey (SLBH) on medically important Gram-negative and positive bacteria. The A1AgNPs were synthesized following the reduction of silver nitrates by sodium citrate. Using SLBH 20 % (v⁄v) stock solution, a dilution series from 1 % (v⁄v) intervals between 0 % and 10 % were made. The agar plates that contain A1AgNPs and SLBH were separately inoculated using bacterial suspension. Each organism's fractional inhibitory concentration index (ΣFIC) was calculated to assess the synergy. The minimum inhibitory concentration (MIC) of A1AgNPs for E. coli (6.7 ± 0.48), Shigella dysenteriae (6.66 ± 0.51), P. vulgaris (7.16 ± 0.4), P. aeruginosa (7.1 ± 0.33) and Salmonella Typhimurium (7.8 ± 0.83) were given in v/v%. Once the addition of equal volumes of A1AgNPs and SLBH, the spectrum of activity of the A1AgNPs improved from Gram-negative spectrum to Gram-positive, including MRSA (MIC 4.31 ± 0.47). Also, MIC values were further reduced, indicating a synergistic activity between the A1AgNPs and SLBH and E. coli, S. dysenteriae, P. vulgaris, P. aeruginosa, and Salmonella Typhimurium ΣFIC was 0.48, 0.48, 0.48, 0.48, and 0.44, respectively. In the future, SLBH can be used as a base for synthetic A1AgNPs to treat pathogenic bacteria.http://www.sciencedirect.com/science/article/pii/S2950194625001414Silver nanoparticles: Sri Lankan Bee HoneyMulti-drug resistant Gram-Negative bacteriaMRSASynergism |
| spellingShingle | Kithsiri Senanayake Wikum Widuranga Kumbukgolla Jayaweera Arachchige Asela Sampath Jayaweera The synergy between Sri Lankan bee honey and silver nanoparticles on pathogenic bacteria The Microbe Silver nanoparticles: Sri Lankan Bee Honey Multi-drug resistant Gram-Negative bacteria MRSA Synergism |
| title | The synergy between Sri Lankan bee honey and silver nanoparticles on pathogenic bacteria |
| title_full | The synergy between Sri Lankan bee honey and silver nanoparticles on pathogenic bacteria |
| title_fullStr | The synergy between Sri Lankan bee honey and silver nanoparticles on pathogenic bacteria |
| title_full_unstemmed | The synergy between Sri Lankan bee honey and silver nanoparticles on pathogenic bacteria |
| title_short | The synergy between Sri Lankan bee honey and silver nanoparticles on pathogenic bacteria |
| title_sort | synergy between sri lankan bee honey and silver nanoparticles on pathogenic bacteria |
| topic | Silver nanoparticles: Sri Lankan Bee Honey Multi-drug resistant Gram-Negative bacteria MRSA Synergism |
| url | http://www.sciencedirect.com/science/article/pii/S2950194625001414 |
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