A Photo-Controllable DNAzyme-Based Nanosensor for miRNA Imaging in Living Cells

MircroRNA (miRNA) exhibits abnormal expression in many cancer diseases, and the detection and analysis of miRNA are significant for the early diagnosis of diseases and research on miRNA functions. In this work, we construct a UV-triggered DNAzyme (UTD) nanosensor for the early detection of miRNA in...

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Main Authors: Yanfei Zhang, Yiling Zhang, Runqi Ouyang, Zong Dai, Si-Yang Liu
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Chemosensors
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Online Access:https://www.mdpi.com/2227-9040/13/4/123
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author Yanfei Zhang
Yiling Zhang
Runqi Ouyang
Zong Dai
Si-Yang Liu
author_facet Yanfei Zhang
Yiling Zhang
Runqi Ouyang
Zong Dai
Si-Yang Liu
author_sort Yanfei Zhang
collection DOAJ
description MircroRNA (miRNA) exhibits abnormal expression in many cancer diseases, and the detection and analysis of miRNA are significant for the early diagnosis of diseases and research on miRNA functions. In this work, we construct a UV-triggered DNAzyme (UTD) nanosensor for the early detection of miRNA in tumor cells. As the nanodevice was delivered into cells and irradiated by UV light, the controllable imaging of miRNA in living cells was achieved. This method effectively avoids false signal issues, providing a new strategy for high-spatiotemporal-resolution imaging of miRNA in living cells.
format Article
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issn 2227-9040
language English
publishDate 2025-04-01
publisher MDPI AG
record_format Article
series Chemosensors
spelling doaj-art-c775544e724148f39b69a754f019d6132025-08-20T03:14:15ZengMDPI AGChemosensors2227-90402025-04-0113412310.3390/chemosensors13040123A Photo-Controllable DNAzyme-Based Nanosensor for miRNA Imaging in Living CellsYanfei Zhang0Yiling Zhang1Runqi Ouyang2Zong Dai3Si-Yang Liu4Guangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen Campus of Sun Yat-Sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen Campus of Sun Yat-Sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen Campus of Sun Yat-Sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen Campus of Sun Yat-Sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen Campus of Sun Yat-Sen University, Shenzhen 518107, ChinaMircroRNA (miRNA) exhibits abnormal expression in many cancer diseases, and the detection and analysis of miRNA are significant for the early diagnosis of diseases and research on miRNA functions. In this work, we construct a UV-triggered DNAzyme (UTD) nanosensor for the early detection of miRNA in tumor cells. As the nanodevice was delivered into cells and irradiated by UV light, the controllable imaging of miRNA in living cells was achieved. This method effectively avoids false signal issues, providing a new strategy for high-spatiotemporal-resolution imaging of miRNA in living cells.https://www.mdpi.com/2227-9040/13/4/123DNAzymeMiRNA detectionphoto-controllablecell imaging
spellingShingle Yanfei Zhang
Yiling Zhang
Runqi Ouyang
Zong Dai
Si-Yang Liu
A Photo-Controllable DNAzyme-Based Nanosensor for miRNA Imaging in Living Cells
Chemosensors
DNAzyme
MiRNA detection
photo-controllable
cell imaging
title A Photo-Controllable DNAzyme-Based Nanosensor for miRNA Imaging in Living Cells
title_full A Photo-Controllable DNAzyme-Based Nanosensor for miRNA Imaging in Living Cells
title_fullStr A Photo-Controllable DNAzyme-Based Nanosensor for miRNA Imaging in Living Cells
title_full_unstemmed A Photo-Controllable DNAzyme-Based Nanosensor for miRNA Imaging in Living Cells
title_short A Photo-Controllable DNAzyme-Based Nanosensor for miRNA Imaging in Living Cells
title_sort photo controllable dnazyme based nanosensor for mirna imaging in living cells
topic DNAzyme
MiRNA detection
photo-controllable
cell imaging
url https://www.mdpi.com/2227-9040/13/4/123
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