CircRNA SEC24A promotes osteoarthritis through miR-107-5p/CASP3 axis
Background: Osteoarthritis (OA) is the most frequently diagnosed chronic joint disease. CircSEC24A is significantly elevated in OA chondrocytes upon IL-1β stimulation. However, its biological function in OA is still not fully understood. Methods: The circRNAs-miRNA-mRNA network was predicted by bioi...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320424000737 |
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| author | Tuerxunjiang Dadihanc Yong Zhang Guo-Qing Li Hai-Kang Zhou Jingyong Huang Xue Zhang Zhi-Qiang Li Hai-Rong Ma |
| author_facet | Tuerxunjiang Dadihanc Yong Zhang Guo-Qing Li Hai-Kang Zhou Jingyong Huang Xue Zhang Zhi-Qiang Li Hai-Rong Ma |
| author_sort | Tuerxunjiang Dadihanc |
| collection | DOAJ |
| description | Background: Osteoarthritis (OA) is the most frequently diagnosed chronic joint disease. CircSEC24A is significantly elevated in OA chondrocytes upon IL-1β stimulation. However, its biological function in OA is still not fully understood. Methods: The circRNAs-miRNA-mRNA network was predicted by bioinformatics analysis. An in vitro OA chondrocytes model was established by IL-1β stimulation. The expression of circSEC24A, miR-107-5p, CASP3, apoptosis-related molecules and extracellular matrix (ECM) components were detected by Western blot and qRT-PCR. MTT assay and Annexin V/PI staining were employed to monitor cell viability and apoptosis, respectively. The interaction between circSEC24A and miR-107-5p, as well as the binding between miR-107-5p and CASP3 3’ UTR were detected by luciferase reporter and RIP assays. Cytokine secretion was monitored by ELISA assay. The role of circSEC24A was also explored in anterior cruciate ligament transection (ACLT) rat models. Results: CircSEC24A and CASP3 were increased, but miR-107-5p was decreased in rat OA cartilage tissues and OA chondrocytes. CircSEC24A acted as a sponge of miR-107-5p. Knockdown of circSEC24A promoted chondrocyte proliferation, but suppressed chondrocyte apoptosis, ECM degradation and inflammation via sponging miR-107-5p. CASP3 was identified as a miR-107-5p target gene. MiR-107-5p mimics protected against OA progression via targeting CASP3. Silencing of circSEC24A alleviated OA progression in ACLT model. Conclusion: CircSEC24A promotes OA progression through miR-107-5p/CASP3 axis. |
| format | Article |
| id | doaj-art-c76c51ca452241af8c311fc52b0f0e88 |
| institution | DOAJ |
| issn | 2352-3204 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-c76c51ca452241af8c311fc52b0f0e882025-08-20T02:48:58ZengElsevierRegenerative Therapy2352-32042024-06-0126607010.1016/j.reth.2024.04.011CircRNA SEC24A promotes osteoarthritis through miR-107-5p/CASP3 axisTuerxunjiang Dadihanc0Yong Zhang1Guo-Qing Li2Hai-Kang Zhou3Jingyong Huang4Xue Zhang5Zhi-Qiang Li6Hai-Rong Ma7State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Province, PR China; Department of Orthopaedic Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Province, PR ChinaSchool of Life Science and Technology, Shanghai Jiao Tong University, Shanghai 200010, PR ChinaDepartment of Orthopaedic Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Province, PR ChinaDepartment of Orthopaedic Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Province, PR ChinaDepartment of Orthopaedic Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Province, PR ChinaState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Province, PR ChinaAnimal Research Center, Xinjiang Medical University, Urumqi 830054, Xinjiang Province, PR ChinaState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Province, PR China; Corresponding author.Background: Osteoarthritis (OA) is the most frequently diagnosed chronic joint disease. CircSEC24A is significantly elevated in OA chondrocytes upon IL-1β stimulation. However, its biological function in OA is still not fully understood. Methods: The circRNAs-miRNA-mRNA network was predicted by bioinformatics analysis. An in vitro OA chondrocytes model was established by IL-1β stimulation. The expression of circSEC24A, miR-107-5p, CASP3, apoptosis-related molecules and extracellular matrix (ECM) components were detected by Western blot and qRT-PCR. MTT assay and Annexin V/PI staining were employed to monitor cell viability and apoptosis, respectively. The interaction between circSEC24A and miR-107-5p, as well as the binding between miR-107-5p and CASP3 3’ UTR were detected by luciferase reporter and RIP assays. Cytokine secretion was monitored by ELISA assay. The role of circSEC24A was also explored in anterior cruciate ligament transection (ACLT) rat models. Results: CircSEC24A and CASP3 were increased, but miR-107-5p was decreased in rat OA cartilage tissues and OA chondrocytes. CircSEC24A acted as a sponge of miR-107-5p. Knockdown of circSEC24A promoted chondrocyte proliferation, but suppressed chondrocyte apoptosis, ECM degradation and inflammation via sponging miR-107-5p. CASP3 was identified as a miR-107-5p target gene. MiR-107-5p mimics protected against OA progression via targeting CASP3. Silencing of circSEC24A alleviated OA progression in ACLT model. Conclusion: CircSEC24A promotes OA progression through miR-107-5p/CASP3 axis.http://www.sciencedirect.com/science/article/pii/S2352320424000737OsteoarthritisChondrocytecircSEC24AmiR-107-5pCASP3 |
| spellingShingle | Tuerxunjiang Dadihanc Yong Zhang Guo-Qing Li Hai-Kang Zhou Jingyong Huang Xue Zhang Zhi-Qiang Li Hai-Rong Ma CircRNA SEC24A promotes osteoarthritis through miR-107-5p/CASP3 axis Regenerative Therapy Osteoarthritis Chondrocyte circSEC24A miR-107-5p CASP3 |
| title | CircRNA SEC24A promotes osteoarthritis through miR-107-5p/CASP3 axis |
| title_full | CircRNA SEC24A promotes osteoarthritis through miR-107-5p/CASP3 axis |
| title_fullStr | CircRNA SEC24A promotes osteoarthritis through miR-107-5p/CASP3 axis |
| title_full_unstemmed | CircRNA SEC24A promotes osteoarthritis through miR-107-5p/CASP3 axis |
| title_short | CircRNA SEC24A promotes osteoarthritis through miR-107-5p/CASP3 axis |
| title_sort | circrna sec24a promotes osteoarthritis through mir 107 5p casp3 axis |
| topic | Osteoarthritis Chondrocyte circSEC24A miR-107-5p CASP3 |
| url | http://www.sciencedirect.com/science/article/pii/S2352320424000737 |
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