A microfluidic system for dynamic yeast cell imaging
The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2008-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112673 |
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| _version_ | 1850152427651596288 |
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| author | Philip J. Lee Noah C. Helman Wendell A. Lim Paul J. Hung |
| author_facet | Philip J. Lee Noah C. Helman Wendell A. Lim Paul J. Hung |
| author_sort | Philip J. Lee |
| collection | DOAJ |
| description | The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae. |
| format | Article |
| id | doaj-art-c7637a6f65d74f83b623346401d830e9 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-c7637a6f65d74f83b623346401d830e92025-08-20T02:25:59ZengTaylor & Francis GroupBioTechniques0736-62051940-98182008-01-01441919510.2144/000112673A microfluidic system for dynamic yeast cell imagingPhilip J. Lee0Noah C. Helman1Wendell A. Lim2Paul J. Hung31CellASIC Corporation, San Leandro2Department of Cellular and Molecular Pharmacology, UC San Francisco, San Francisco, CA, USA2Department of Cellular and Molecular Pharmacology, UC San Francisco, San Francisco, CA, USA1CellASIC Corporation, San LeandroThe investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae.https://www.future-science.com/doi/10.2144/000112673 |
| spellingShingle | Philip J. Lee Noah C. Helman Wendell A. Lim Paul J. Hung A microfluidic system for dynamic yeast cell imaging BioTechniques |
| title | A microfluidic system for dynamic yeast cell imaging |
| title_full | A microfluidic system for dynamic yeast cell imaging |
| title_fullStr | A microfluidic system for dynamic yeast cell imaging |
| title_full_unstemmed | A microfluidic system for dynamic yeast cell imaging |
| title_short | A microfluidic system for dynamic yeast cell imaging |
| title_sort | microfluidic system for dynamic yeast cell imaging |
| url | https://www.future-science.com/doi/10.2144/000112673 |
| work_keys_str_mv | AT philipjlee amicrofluidicsystemfordynamicyeastcellimaging AT noahchelman amicrofluidicsystemfordynamicyeastcellimaging AT wendellalim amicrofluidicsystemfordynamicyeastcellimaging AT pauljhung amicrofluidicsystemfordynamicyeastcellimaging AT philipjlee microfluidicsystemfordynamicyeastcellimaging AT noahchelman microfluidicsystemfordynamicyeastcellimaging AT wendellalim microfluidicsystemfordynamicyeastcellimaging AT pauljhung microfluidicsystemfordynamicyeastcellimaging |