Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods
This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Mo...
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Hasan Eleroğlu
2020-12-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4038 |
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| author | Mohammad Afzal Hossain Nadia Khan Disha Jahid Hasan Shourove Pappu Dey |
| author_facet | Mohammad Afzal Hossain Nadia Khan Disha Jahid Hasan Shourove Pappu Dey |
| author_sort | Mohammad Afzal Hossain |
| collection | DOAJ |
| description | This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used. |
| format | Article |
| id | doaj-art-c75eec29e01e42c6b2cfc185f2ca7fb2 |
| institution | DOAJ |
| issn | 2148-127X |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-c75eec29e01e42c6b2cfc185f2ca7fb22025-08-20T03:05:14ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2020-12-018122749275510.24925/turjaf.v8i12.2749-2755.40381931Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction MethodsMohammad Afzal Hossain0Nadia Khan Disha1Jahid Hasan Shourove2Pappu Dey3Department of Food Engineering and Tea Technology, Shahjalal University of Science and Technology, Sylhet-3114Department of Food Engineering and Tea Technology, Shahjalal University of Science and Technology, Sylhet-3114Department of Food Engineering and Tea Technology, Shahjalal University of Science and Technology, Sylhet-3114Department of Food Engineering and Tea Technology, Shahjalal University of Science and Technology, Sylhet-3114This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4038antioxidant activitytotal tanninssolvent extractionextraction timeextraction temperature |
| spellingShingle | Mohammad Afzal Hossain Nadia Khan Disha Jahid Hasan Shourove Pappu Dey Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods Turkish Journal of Agriculture: Food Science and Technology antioxidant activity total tannins solvent extraction extraction time extraction temperature |
| title | Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods |
| title_full | Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods |
| title_fullStr | Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods |
| title_full_unstemmed | Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods |
| title_short | Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods |
| title_sort | determination of antioxidant activity and total tannin from drumstick moringa oleifera lam leaves using different solvent extraction methods |
| topic | antioxidant activity total tannins solvent extraction extraction time extraction temperature |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4038 |
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