Evaluation of Clinical and Immunopathological Features of Different Infective Doses of Trypanosoma cruzi in Dogs during the Acute Phase
Infection with Trypanosoma cruzi is a major risk in Latin America, and dogs are believed to be good models for evaluating Chagas disease. Here, we evaluated the clinical and immunopathological alterations developed by mongrel dogs experimentally infected with different infective doses (2,000, 20,000...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/635169 |
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| author | Israel A. Quijano-Hernández Alejandro Castro-Barcena Esteban Aparicio-Burgos Marco A. Barbosa-Mireles Julio V. Cruz-Chan Juan C. Vázquez-Chagoyán Manuel E. Bolio-González Eric Dumonteil |
| author_facet | Israel A. Quijano-Hernández Alejandro Castro-Barcena Esteban Aparicio-Burgos Marco A. Barbosa-Mireles Julio V. Cruz-Chan Juan C. Vázquez-Chagoyán Manuel E. Bolio-González Eric Dumonteil |
| author_sort | Israel A. Quijano-Hernández |
| collection | DOAJ |
| description | Infection with Trypanosoma cruzi is a major risk in Latin America, and dogs are believed to be good models for evaluating Chagas disease. Here, we evaluated the clinical and immunopathological alterations developed by mongrel dogs experimentally infected with different infective doses (2,000, 20,000, and 200,000 metacyclic trypomastigotes of Sylvio X10/4 strain kg−1 via intraperitoneal). Clinical and electrocardiographic parameters, as well as antibody production and pathologic lesions were evaluated. All three doses of this strain of T. cruzi induced a similar pattern of infection characterized by cardiac arrhythmias and severe and diffuse myocarditis. Specific anti-T. cruzi IgG indicated seroconversion by day 14 after infection, and IgG levels increased during the period of evaluation. Mortality was observed only in dogs infected with the medium or high parasite doses, but not in the group infected with a low dose of 2,000 parasites kg-1. Infection with a low dose of parasites provides an excellent nonlethal model to evaluate the immunopathology of the acute disease in dogs infected with the Sylvio X10/4 strain of T. cruzi. |
| format | Article |
| id | doaj-art-c75c4f765e5243739d83bfb45db67d7d |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-c75c4f765e5243739d83bfb45db67d7d2025-08-20T02:20:05ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/635169635169Evaluation of Clinical and Immunopathological Features of Different Infective Doses of Trypanosoma cruzi in Dogs during the Acute PhaseIsrael A. Quijano-Hernández0Alejandro Castro-Barcena1Esteban Aparicio-Burgos2Marco A. Barbosa-Mireles3Julio V. Cruz-Chan4Juan C. Vázquez-Chagoyán5Manuel E. Bolio-González6Eric Dumonteil7Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Cerrillo Piedras Blancas s/n, 50200 Toluca, MexicoFacultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Cerrillo Piedras Blancas s/n, 50200 Toluca, MexicoFacultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Cerrillo Piedras Blancas s/n, 50200 Toluca, MexicoFacultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Cerrillo Piedras Blancas s/n, 50200 Toluca, MexicoLaboratorio de Parasitología, Centro de Investigaciones Regionales “Hideyo Noguchi”, Universidad Autónoma de Yucatán, 97000 Mérida, MexicoFacultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Cerrillo Piedras Blancas s/n, 50200 Toluca, MexicoFacultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Carretera Mérida-Xmatkuil, km 15, 97315 Mérida, MexicoLaboratorio de Parasitología, Centro de Investigaciones Regionales “Hideyo Noguchi”, Universidad Autónoma de Yucatán, 97000 Mérida, MexicoInfection with Trypanosoma cruzi is a major risk in Latin America, and dogs are believed to be good models for evaluating Chagas disease. Here, we evaluated the clinical and immunopathological alterations developed by mongrel dogs experimentally infected with different infective doses (2,000, 20,000, and 200,000 metacyclic trypomastigotes of Sylvio X10/4 strain kg−1 via intraperitoneal). Clinical and electrocardiographic parameters, as well as antibody production and pathologic lesions were evaluated. All three doses of this strain of T. cruzi induced a similar pattern of infection characterized by cardiac arrhythmias and severe and diffuse myocarditis. Specific anti-T. cruzi IgG indicated seroconversion by day 14 after infection, and IgG levels increased during the period of evaluation. Mortality was observed only in dogs infected with the medium or high parasite doses, but not in the group infected with a low dose of 2,000 parasites kg-1. Infection with a low dose of parasites provides an excellent nonlethal model to evaluate the immunopathology of the acute disease in dogs infected with the Sylvio X10/4 strain of T. cruzi.http://dx.doi.org/10.1100/2012/635169 |
| spellingShingle | Israel A. Quijano-Hernández Alejandro Castro-Barcena Esteban Aparicio-Burgos Marco A. Barbosa-Mireles Julio V. Cruz-Chan Juan C. Vázquez-Chagoyán Manuel E. Bolio-González Eric Dumonteil Evaluation of Clinical and Immunopathological Features of Different Infective Doses of Trypanosoma cruzi in Dogs during the Acute Phase The Scientific World Journal |
| title | Evaluation of Clinical and Immunopathological Features of Different Infective Doses of Trypanosoma cruzi in Dogs during the Acute Phase |
| title_full | Evaluation of Clinical and Immunopathological Features of Different Infective Doses of Trypanosoma cruzi in Dogs during the Acute Phase |
| title_fullStr | Evaluation of Clinical and Immunopathological Features of Different Infective Doses of Trypanosoma cruzi in Dogs during the Acute Phase |
| title_full_unstemmed | Evaluation of Clinical and Immunopathological Features of Different Infective Doses of Trypanosoma cruzi in Dogs during the Acute Phase |
| title_short | Evaluation of Clinical and Immunopathological Features of Different Infective Doses of Trypanosoma cruzi in Dogs during the Acute Phase |
| title_sort | evaluation of clinical and immunopathological features of different infective doses of trypanosoma cruzi in dogs during the acute phase |
| url | http://dx.doi.org/10.1100/2012/635169 |
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