Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens
Objective This study evaluated the effects of dietary sodium sulfate (Na2SO4) supplementation on eggshell quality, uterine ion transportation, and glycosaminoglycan (GAG) synthesis. Methods A total of 432 48-wk-old Hy-line Brown laying hens were randomly divided into 6 dietary treatments with 8 repl...
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Asian-Australasian Association of Animal Production Societies
2025-05-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0456.pdf |
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| author | Kai-bo Fu Dong Dai Jian-min Zhou Jing Wang Hai-jun Zhang Shu-geng Wu Guang-hai Qi Jing Wang |
| author_facet | Kai-bo Fu Dong Dai Jian-min Zhou Jing Wang Hai-jun Zhang Shu-geng Wu Guang-hai Qi Jing Wang |
| author_sort | Kai-bo Fu |
| collection | DOAJ |
| description | Objective This study evaluated the effects of dietary sodium sulfate (Na2SO4) supplementation on eggshell quality, uterine ion transportation, and glycosaminoglycan (GAG) synthesis. Methods A total of 432 48-wk-old Hy-line Brown laying hens were randomly divided into 6 dietary treatments with 8 replicates of 9 birds each. The experimental laying hens were fed the corn-soybean meal diets (containing 0.15% NaCl) supplemented with 0.22%, 0.37%, 0.52%, 0.68%, 0.83%, or 0.99% Na2SO4 for 12 weeks. Results Results showed that the eggshell breaking strength and eggshell ratio significantly increased in the 0.68% Na2SO4 group at the end of wk 56 and wk 60 (p<0.05). In addition, eggshell thickness and weight significantly increased in the 0.68% Na2SO4 group at the end of wk 60 (p<0.05). Eggshell calcium content in the 0.68% Na2SO4 group was higher than that of 0.22% and 0.99% groups (p<0.001). The concentrations of K+ and Ca2+ in the uterine fluid were significantly greater in the 0.68% group than in the other groups (p<0.05). Dietary Na2SO4 increased the gene expression of SLC8A1, SCNN1A, ATP1B1, and KCNMA1 quadratically in the uterus (p<0.05), and higher values were observed in 0.68% group. Additionally, the GAG contents of the eggshell, and ATP-sulfurylase, sulfotransferase, chondroitin sulfate, and dermatan sulfate contents of the isthmus increased linearly with the increment of dietary Na2SO4 (p<0.05). There was a remarkable reduction in mammillary knob width, mammillary thickness, and the percentage of the mammillary layer (p<0.05), and an increment in mammillary knob density, effective thickness, and total thickness in the 0.68% group compared with the 0.22% and 0.99% groups (p<0.05). Conclusion Overall, there was no dose-related difference with the increment of dietary Na2SO4 levels. The addition of 0.68% Na2SO4 in the corn-soybean basal diet (0.15% Cl) regulated uterine ion transport, increased GAG contents of eggshell, and improved eggshell ultrastructure and quality. |
| format | Article |
| id | doaj-art-c7599058bb0d4c37a209d1a7dbaba25f |
| institution | Kabale University |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-c7599058bb0d4c37a209d1a7dbaba25f2025-08-20T03:53:07ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-05-013851029104010.5713/ab.24.045625384Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hensKai-bo Fu0Dong Dai1Jian-min Zhou2Jing Wang3Hai-jun Zhang4Shu-geng Wu5Guang-hai Qi6Jing Wang7 Key Laboratory of Feed Biotechnology of Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China Key Laboratory of Feed Biotechnology of Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China Key Laboratory of Feed Biotechnology of Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China Key Laboratory of Feed Biotechnology of Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China Key Laboratory of Feed Biotechnology of Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China Key Laboratory of Feed Biotechnology of Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China Key Laboratory of Feed Biotechnology of Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China Key Laboratory of Feed Biotechnology of Ministry of Agriculture & Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaObjective This study evaluated the effects of dietary sodium sulfate (Na2SO4) supplementation on eggshell quality, uterine ion transportation, and glycosaminoglycan (GAG) synthesis. Methods A total of 432 48-wk-old Hy-line Brown laying hens were randomly divided into 6 dietary treatments with 8 replicates of 9 birds each. The experimental laying hens were fed the corn-soybean meal diets (containing 0.15% NaCl) supplemented with 0.22%, 0.37%, 0.52%, 0.68%, 0.83%, or 0.99% Na2SO4 for 12 weeks. Results Results showed that the eggshell breaking strength and eggshell ratio significantly increased in the 0.68% Na2SO4 group at the end of wk 56 and wk 60 (p<0.05). In addition, eggshell thickness and weight significantly increased in the 0.68% Na2SO4 group at the end of wk 60 (p<0.05). Eggshell calcium content in the 0.68% Na2SO4 group was higher than that of 0.22% and 0.99% groups (p<0.001). The concentrations of K+ and Ca2+ in the uterine fluid were significantly greater in the 0.68% group than in the other groups (p<0.05). Dietary Na2SO4 increased the gene expression of SLC8A1, SCNN1A, ATP1B1, and KCNMA1 quadratically in the uterus (p<0.05), and higher values were observed in 0.68% group. Additionally, the GAG contents of the eggshell, and ATP-sulfurylase, sulfotransferase, chondroitin sulfate, and dermatan sulfate contents of the isthmus increased linearly with the increment of dietary Na2SO4 (p<0.05). There was a remarkable reduction in mammillary knob width, mammillary thickness, and the percentage of the mammillary layer (p<0.05), and an increment in mammillary knob density, effective thickness, and total thickness in the 0.68% group compared with the 0.22% and 0.99% groups (p<0.05). Conclusion Overall, there was no dose-related difference with the increment of dietary Na2SO4 levels. The addition of 0.68% Na2SO4 in the corn-soybean basal diet (0.15% Cl) regulated uterine ion transport, increased GAG contents of eggshell, and improved eggshell ultrastructure and quality.http://www.animbiosci.org/upload/pdf/ab-24-0456.pdfeggshell qualityglycosaminoglycanlaying hensodium sulfateuterine ion transportation |
| spellingShingle | Kai-bo Fu Dong Dai Jian-min Zhou Jing Wang Hai-jun Zhang Shu-geng Wu Guang-hai Qi Jing Wang Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens Animal Bioscience eggshell quality glycosaminoglycan laying hen sodium sulfate uterine ion transportation |
| title | Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens |
| title_full | Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens |
| title_fullStr | Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens |
| title_full_unstemmed | Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens |
| title_short | Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens |
| title_sort | dietary sodium sulfate supplementation improves eggshell quality uterine ion transportation and glycosaminoglycan synthesis in laying hens |
| topic | eggshell quality glycosaminoglycan laying hen sodium sulfate uterine ion transportation |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0456.pdf |
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