Effects of different lactic acid bacteria fermentation on active substances and functional characteristics of honeysuckle liquid: analysis of metabolites of honeysuckle liquid based on metabolomics

In order to study the effects of lactic acid bacteria on the active substances and functions of honeysuckle, metabolomics was used to analyze the differential metabolites of honeysuckle fermented by lactic acid bacteria. The results showed that Lactobacillus acidophilus increased the contents of tot...

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Main Authors: Jing Yang, Hanhan Shi, Lili Cai, Sensen Zhang, Yuanzeng Zhao, Xiaojia Su, Haiyan Sun, Yinping Cao, Yongchao Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1595351/full
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Summary:In order to study the effects of lactic acid bacteria on the active substances and functions of honeysuckle, metabolomics was used to analyze the differential metabolites of honeysuckle fermented by lactic acid bacteria. The results showed that Lactobacillus acidophilus increased the contents of total phenols, flavonoids and chlorogenic acid by 26.48, 22.59, and 33.57%, respectively, compared with Control Group (CK). The antioxidant capacity and α-glucosidase activity of fermented honeysuckle liquid (FLJT) were the strongest, and the scavenging activity and reducing ability of DPPH radical, hydroxyl radical and superoxide anion radical increased by 33.96, 25.60, 58.21 and 39.29%, respectively, compared with unfermented honeysuckle liquid without lactic acid bacteria (LJT). The 991 metabolites with significant differences were screened and enriched into 79 metabolic pathways. The contents of 4-hydroxybenzoic acid, 3, 4-dihydroxyhydrocinnamic acid, chlorogenic acid and 4-hydroxycinnamic acid, which have antioxidant and hypoglycemic activities, and flavonoids in FLJT were increased compared with those in LJT. Lactobacillus acidophilus fermentation could promote the synthesis and accumulation of secondary metabolites in honeysuckle liquid. This study provides a theoretical basis for the development of honeysuckle functional products.
ISSN:1664-302X