Sperm DNA damage and disturbed chromatin condensation indexes (DFI and CMA3) in normozoospermic men with unexplained infertility problem
Purpose The quantitatively measured sperm DNA damage and disturbed chromatin condensation indexes (%DNA fragmentation index [DFI%] and %high DNA staining index [%HDS]) and their relationships with sperm quality in normospermic men with unexplained infertility were investigated. The aim was also high...
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Taylor & Francis Group
2025-12-01
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| Series: | The Aging Male |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13685538.2025.2472774 |
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| author | Mehdi Abedinzadeh Sara Abedinzadeh Fatemeh Sadeghi-Nodoushan Fatemeh Pourrajab |
| author_facet | Mehdi Abedinzadeh Sara Abedinzadeh Fatemeh Sadeghi-Nodoushan Fatemeh Pourrajab |
| author_sort | Mehdi Abedinzadeh |
| collection | DOAJ |
| description | Purpose The quantitatively measured sperm DNA damage and disturbed chromatin condensation indexes (%DNA fragmentation index [DFI%] and %high DNA staining index [%HDS]) and their relationships with sperm quality in normospermic men with unexplained infertility were investigated. The aim was also highlighting the impact of age on both DFI and CMA3 staining and on sperm quality, and their associations with male infertility.Methods In this retrospective study, conventional semen tests, including sperm motility and morphological evaluations and DFI and disturbed chromatin condensation indexes (DFI, CMA3) were performed according to the World Health Organization (WHO) 2021 criteria. DFI and CMA3 were evaluated using sperm chromatin dispersion (SCD) and chromomycin A3 (CMA3) staining assays and then correlation and regression analysis were done.Results By analyzing SCD and CMA3 results, notable differences were found in sperm parameters among different DFI and CMA3 groups (all p < 0.05). It was found that in the male fertility quality sperm concentration, progressive (PR)/non-progressive (NP) motility, immobility, and morphology were significantly associated with sperm DFI and CMA3, but not with age (p < 0.05).Conclusions Sperm molecular index DFI and CMA3 negatively affect male fertility quality through semen parameters (sperm concentration, motility, and morphology). SCD and CMA3 indexes show a significant negative correlation with sperm quality in normospermic males, which highlights its role in the assessment of male fertility potential and molecular evaluation of infertility treatment. |
| format | Article |
| id | doaj-art-c74ff213ceec4c4dbc822f1aef5ee357 |
| institution | OA Journals |
| issn | 1368-5538 1473-0790 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | The Aging Male |
| spelling | doaj-art-c74ff213ceec4c4dbc822f1aef5ee3572025-08-20T02:38:11ZengTaylor & Francis GroupThe Aging Male1368-55381473-07902025-12-0128110.1080/13685538.2025.2472774Sperm DNA damage and disturbed chromatin condensation indexes (DFI and CMA3) in normozoospermic men with unexplained infertility problemMehdi Abedinzadeh0Sara Abedinzadeh1Fatemeh Sadeghi-Nodoushan2Fatemeh Pourrajab3Department of Urology, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Nutrition, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Nutrition, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, IranReproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranPurpose The quantitatively measured sperm DNA damage and disturbed chromatin condensation indexes (%DNA fragmentation index [DFI%] and %high DNA staining index [%HDS]) and their relationships with sperm quality in normospermic men with unexplained infertility were investigated. The aim was also highlighting the impact of age on both DFI and CMA3 staining and on sperm quality, and their associations with male infertility.Methods In this retrospective study, conventional semen tests, including sperm motility and morphological evaluations and DFI and disturbed chromatin condensation indexes (DFI, CMA3) were performed according to the World Health Organization (WHO) 2021 criteria. DFI and CMA3 were evaluated using sperm chromatin dispersion (SCD) and chromomycin A3 (CMA3) staining assays and then correlation and regression analysis were done.Results By analyzing SCD and CMA3 results, notable differences were found in sperm parameters among different DFI and CMA3 groups (all p < 0.05). It was found that in the male fertility quality sperm concentration, progressive (PR)/non-progressive (NP) motility, immobility, and morphology were significantly associated with sperm DFI and CMA3, but not with age (p < 0.05).Conclusions Sperm molecular index DFI and CMA3 negatively affect male fertility quality through semen parameters (sperm concentration, motility, and morphology). SCD and CMA3 indexes show a significant negative correlation with sperm quality in normospermic males, which highlights its role in the assessment of male fertility potential and molecular evaluation of infertility treatment.https://www.tandfonline.com/doi/10.1080/13685538.2025.2472774DNA fragmentation index [DFI]high DNA staining [HDS]chromomycin A3 [CMA3]infertilitynormospermia |
| spellingShingle | Mehdi Abedinzadeh Sara Abedinzadeh Fatemeh Sadeghi-Nodoushan Fatemeh Pourrajab Sperm DNA damage and disturbed chromatin condensation indexes (DFI and CMA3) in normozoospermic men with unexplained infertility problem The Aging Male DNA fragmentation index [DFI] high DNA staining [HDS] chromomycin A3 [CMA3] infertility normospermia |
| title | Sperm DNA damage and disturbed chromatin condensation indexes (DFI and CMA3) in normozoospermic men with unexplained infertility problem |
| title_full | Sperm DNA damage and disturbed chromatin condensation indexes (DFI and CMA3) in normozoospermic men with unexplained infertility problem |
| title_fullStr | Sperm DNA damage and disturbed chromatin condensation indexes (DFI and CMA3) in normozoospermic men with unexplained infertility problem |
| title_full_unstemmed | Sperm DNA damage and disturbed chromatin condensation indexes (DFI and CMA3) in normozoospermic men with unexplained infertility problem |
| title_short | Sperm DNA damage and disturbed chromatin condensation indexes (DFI and CMA3) in normozoospermic men with unexplained infertility problem |
| title_sort | sperm dna damage and disturbed chromatin condensation indexes dfi and cma3 in normozoospermic men with unexplained infertility problem |
| topic | DNA fragmentation index [DFI] high DNA staining [HDS] chromomycin A3 [CMA3] infertility normospermia |
| url | https://www.tandfonline.com/doi/10.1080/13685538.2025.2472774 |
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