Genetic diversity and prevalence of group A rotavirus infection in children of Imphal, Manipur, India: A hospital-based surveillance study conducted during December 2015 to March 2019

Rotavirus is the most common cause of acute gastroenteritis in infants and children worldwide. The study is hospital-based surveillance of rotavirus diarrhea in children from Imphal, Manipur, India conducted from December 2015 to March 2019. The positivity rate was found to be high ∼69.25% (358/517)...

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Main Authors: Damayanti Yengkhom Devi, Chongtham Shyamsunder Singh, C. Durga Rao, Nima D. Namsa
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Vaccine: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590136225000087
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Summary:Rotavirus is the most common cause of acute gastroenteritis in infants and children worldwide. The study is hospital-based surveillance of rotavirus diarrhea in children from Imphal, Manipur, India conducted from December 2015 to March 2019. The positivity rate was found to be high ∼69.25% (358/517) and proportion of diarrhea cases and rotavirus diarrhea was peak in winter months and mostly in children from 6 to 24 months. G3 (43%) was the most widely circulating genotype in Imphal followed by G1 (16%), G2 (8%), G9 (5%), G8 (3%), G10 (1%), and G4 (1%), while G12 (0.26%) was rarely detected. Among P-types, P[6] (22%) accounted for the highest prevalence followed by P[8] (11%) and P[4] (4%), P[11] (4%), P[10] (3%), P-type mixed infection 3%, while 53% were untypeable. In G/P combinations, we detected 22 different rotavirus strains at varying frequencies. Globally distributed G3P[8] and G1P[8] strains were observed in the study. G3P[6] emerged as the most predominant rotavirus strain followed by G3P[8], G1P[6], G1P[8], and G9P[6]. The common rotavirus strains distributed across the region namely G3P[8], G1P[8], G2P[4], G9P[4], G1P[4], G1P[6], and G9P[6] were also observed. Interestingly, our study has observed a high percentage of unusual strains namely G9P[4], G1P[11], G2P[11], G3P[10], G3P[11], G4P[11], G9P[10], G9P[11],G10P[6], and G10P[8]. Of note, the high frequency of non-typeable rotavirus P-types (56%) are suggestive of point mutations that might have accumulated in the primer-binding region of VP4 gene. The findings of the present study revealed the hospital-based prevalence of rotavirus disease and the circulating genotypes during the pre-vaccination period and highlights the need for continuous surveillance of rotavirus infection post-rotavac vaccine introduction in the state of Manipur, India.
ISSN:2590-1362