Next-Generation Sequencing Analysis of 3 Uterine Adenosarcomas with Heterogeneously Differentiated Genomic Mutations

Uterine adenosarcoma (UA) is an uncommon mixed tumor containing a benign to at most mildly atypical epithelial component and a sarcoma-like stroma, usually a low-grade, stromal component, with rare heterogeneous elements. Currently, tumor etiology is largely unknown. To better understand the gene mu...

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Main Authors: Yao Li, Xue Meng, Yuqing Luo, Xiang Huang, Shuai Luo, Jinjing Wang
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2023/7436368
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author Yao Li
Xue Meng
Yuqing Luo
Xiang Huang
Shuai Luo
Jinjing Wang
author_facet Yao Li
Xue Meng
Yuqing Luo
Xiang Huang
Shuai Luo
Jinjing Wang
author_sort Yao Li
collection DOAJ
description Uterine adenosarcoma (UA) is an uncommon mixed tumor containing a benign to at most mildly atypical epithelial component and a sarcoma-like stroma, usually a low-grade, stromal component, with rare heterogeneous elements. Currently, tumor etiology is largely unknown. To better understand the gene mutations in UA, next-generation sequencing (NGS) technology analysis was performed. This study showed that two low-grade UAs with heterologous components had ATRX gene frameshift mutation, and one patient had a MED12 missense mutation. Copy number amplification genes were mainly observed on chromosome 12q13–15. In this study, PIK3/AKT/PTEN pathway mutations were found to be common in adenosarcoma. In addition, a rare BCORL1-PRR14L fusion mutation was also identified. These findings provide a basis for future research into these molecular changes in tumorigenesis and targeted therapy.
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publishDate 2023-01-01
publisher Wiley
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series International Journal of Analytical Chemistry
spelling doaj-art-c74ac78e8b1c4d2eb97b6fac35af8f0b2025-08-20T03:22:57ZengWileyInternational Journal of Analytical Chemistry1687-87792023-01-01202310.1155/2023/7436368Next-Generation Sequencing Analysis of 3 Uterine Adenosarcomas with Heterogeneously Differentiated Genomic MutationsYao Li0Xue Meng1Yuqing Luo2Xiang Huang3Shuai Luo4Jinjing Wang5Department of PathologyDepartment of PathologyDepartment of PathologyDepartment of PathologyDepartment of PathologyDepartment of PathologyUterine adenosarcoma (UA) is an uncommon mixed tumor containing a benign to at most mildly atypical epithelial component and a sarcoma-like stroma, usually a low-grade, stromal component, with rare heterogeneous elements. Currently, tumor etiology is largely unknown. To better understand the gene mutations in UA, next-generation sequencing (NGS) technology analysis was performed. This study showed that two low-grade UAs with heterologous components had ATRX gene frameshift mutation, and one patient had a MED12 missense mutation. Copy number amplification genes were mainly observed on chromosome 12q13–15. In this study, PIK3/AKT/PTEN pathway mutations were found to be common in adenosarcoma. In addition, a rare BCORL1-PRR14L fusion mutation was also identified. These findings provide a basis for future research into these molecular changes in tumorigenesis and targeted therapy.http://dx.doi.org/10.1155/2023/7436368
spellingShingle Yao Li
Xue Meng
Yuqing Luo
Xiang Huang
Shuai Luo
Jinjing Wang
Next-Generation Sequencing Analysis of 3 Uterine Adenosarcomas with Heterogeneously Differentiated Genomic Mutations
International Journal of Analytical Chemistry
title Next-Generation Sequencing Analysis of 3 Uterine Adenosarcomas with Heterogeneously Differentiated Genomic Mutations
title_full Next-Generation Sequencing Analysis of 3 Uterine Adenosarcomas with Heterogeneously Differentiated Genomic Mutations
title_fullStr Next-Generation Sequencing Analysis of 3 Uterine Adenosarcomas with Heterogeneously Differentiated Genomic Mutations
title_full_unstemmed Next-Generation Sequencing Analysis of 3 Uterine Adenosarcomas with Heterogeneously Differentiated Genomic Mutations
title_short Next-Generation Sequencing Analysis of 3 Uterine Adenosarcomas with Heterogeneously Differentiated Genomic Mutations
title_sort next generation sequencing analysis of 3 uterine adenosarcomas with heterogeneously differentiated genomic mutations
url http://dx.doi.org/10.1155/2023/7436368
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