Phytochemical Profile and Antioxidant Activity of Juglans regia L. Green Husk Extracts from Tuyserkan Region, Iran
Green husk is a byproduct of the walnut (Juglans regia L.) and could be a potential source of phytochemicals with important bioactivities. The extracts of J. regia L. green husk collected from the Tuyserkan region of Hamedan province were evaluated for their phytochemical profile and antioxidant act...
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North Carolina State University
2025-07-01
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| Online Access: | https://ojs.bioresources.com/index.php/BRJ/article/view/24641 |
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| author | Seyyed Khalil HosseiniHashemi Hadi Baseri Jakia Jerin Mehjabin Md. Khorshed Alam Sayed Khosrow HossinAshrafi Marjan Lotfi |
| author_facet | Seyyed Khalil HosseiniHashemi Hadi Baseri Jakia Jerin Mehjabin Md. Khorshed Alam Sayed Khosrow HossinAshrafi Marjan Lotfi |
| author_sort | Seyyed Khalil HosseiniHashemi |
| collection | DOAJ |
| description | Green husk is a byproduct of the walnut (Juglans regia L.) and could be a potential source of phytochemicals with important bioactivities. The extracts of J. regia L. green husk collected from the Tuyserkan region of Hamedan province were evaluated for their phytochemical profile and antioxidant activities. The chemical composition of crude extracts was analyzed by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). LC-MS analysis of green husk aqueous methanolic extract detected several compounds including phenolic acids, flavonoids, and hydrolyzable tannins. GC-MS analysis of the methanolic extracts revealed the presence of 1,2-dimethylbenzene (3.4%), methyl 14-methylpentadecanoate (2.82%), and methyl stearate (2.7%) as major compounds. The major components identified in the GC-MS analysis of non-polar hexane extract were (23S)-ethylcholest-5-en-3-β-ol (32.2%), δ-tocopherol (16.8%), lupeol (11.8%), and octadecane (5.7%). The antioxidant activity of the crude extracts was evaluated by DPPH assay, which showed aqueous methanol extract to be a more effective antioxidant agent (22.7%) compared to the hexane extract (14%) at the concentration of 1000 ppm. The findings suggest that methanolic extracts of walnut green husks from the Tuyserkan region are rich in bioactive compounds and exhibit more potent antioxidant activity than hexane extracts, demonstrating their potential use in pharmaceutical and food industries. |
| format | Article |
| id | doaj-art-c74758da8eae4ef3a1d2d469ac151509 |
| institution | DOAJ |
| issn | 1930-2126 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj-art-c74758da8eae4ef3a1d2d469ac1515092025-08-20T03:02:26ZengNorth Carolina State UniversityBioResources1930-21262025-07-01203726572772947Phytochemical Profile and Antioxidant Activity of Juglans regia L. Green Husk Extracts from Tuyserkan Region, IranSeyyed Khalil HosseiniHashemi0https://orcid.org/0000-0001-6236-0376Hadi Baseri1https://orcid.org/0000-0002-3893-7881Jakia Jerin Mehjabin2https://orcid.org/0000-0003-3117-7151Md. Khorshed Alam3https://orcid.org/0000-0002-8276-6020Sayed Khosrow HossinAshrafi4https://orcid.org/0000-0002-2800-4455Marjan Lotfi5https://orcid.org/0009-0002-4833-4125Department of Wood Science and Paper Technology, Karaj Branch, Islamic Azad University, Karaj, IranSchool of Chemistry, Damghan University, Damghan, IranFaculty of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, JapanEnvironmental Conservation Division, Environment and Fisheries Applied Research Department, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, JapanDepartment of Wood Science and Paper Technology, Ka.C., Islamic Azad University, Karaj, IranDepartment of Chemistry, Bab.C., Islamic Azad University, Babol, IranGreen husk is a byproduct of the walnut (Juglans regia L.) and could be a potential source of phytochemicals with important bioactivities. The extracts of J. regia L. green husk collected from the Tuyserkan region of Hamedan province were evaluated for their phytochemical profile and antioxidant activities. The chemical composition of crude extracts was analyzed by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). LC-MS analysis of green husk aqueous methanolic extract detected several compounds including phenolic acids, flavonoids, and hydrolyzable tannins. GC-MS analysis of the methanolic extracts revealed the presence of 1,2-dimethylbenzene (3.4%), methyl 14-methylpentadecanoate (2.82%), and methyl stearate (2.7%) as major compounds. The major components identified in the GC-MS analysis of non-polar hexane extract were (23S)-ethylcholest-5-en-3-β-ol (32.2%), δ-tocopherol (16.8%), lupeol (11.8%), and octadecane (5.7%). The antioxidant activity of the crude extracts was evaluated by DPPH assay, which showed aqueous methanol extract to be a more effective antioxidant agent (22.7%) compared to the hexane extract (14%) at the concentration of 1000 ppm. The findings suggest that methanolic extracts of walnut green husks from the Tuyserkan region are rich in bioactive compounds and exhibit more potent antioxidant activity than hexane extracts, demonstrating their potential use in pharmaceutical and food industries.https://ojs.bioresources.com/index.php/BRJ/article/view/24641walnut green huskextractschemical compositionantioxidant activitylc/msgc/ms |
| spellingShingle | Seyyed Khalil HosseiniHashemi Hadi Baseri Jakia Jerin Mehjabin Md. Khorshed Alam Sayed Khosrow HossinAshrafi Marjan Lotfi Phytochemical Profile and Antioxidant Activity of Juglans regia L. Green Husk Extracts from Tuyserkan Region, Iran BioResources walnut green husk extracts chemical composition antioxidant activity lc/ms gc/ms |
| title | Phytochemical Profile and Antioxidant Activity of Juglans regia L. Green Husk Extracts from Tuyserkan Region, Iran |
| title_full | Phytochemical Profile and Antioxidant Activity of Juglans regia L. Green Husk Extracts from Tuyserkan Region, Iran |
| title_fullStr | Phytochemical Profile and Antioxidant Activity of Juglans regia L. Green Husk Extracts from Tuyserkan Region, Iran |
| title_full_unstemmed | Phytochemical Profile and Antioxidant Activity of Juglans regia L. Green Husk Extracts from Tuyserkan Region, Iran |
| title_short | Phytochemical Profile and Antioxidant Activity of Juglans regia L. Green Husk Extracts from Tuyserkan Region, Iran |
| title_sort | phytochemical profile and antioxidant activity of juglans regia l green husk extracts from tuyserkan region iran |
| topic | walnut green husk extracts chemical composition antioxidant activity lc/ms gc/ms |
| url | https://ojs.bioresources.com/index.php/BRJ/article/view/24641 |
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