Amebicidal Activity of <i>Escherichia coli</i> Nissle 1917 Against <i>Entamoeba histolytica</i>
Amebiasis is a globally prevalent infection that can lead to fatal outcomes if not adequately treated. Conventional treatment with imidazoles often fails due to side effects and resistance, emphasizing the need for alternative therapies. The probiotic <i>Escherichia coli</i> Nissle 1917...
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2025-04-01
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| author | Vivian Moura-Oliveira Fabrício M. S. Oliveira Olga L. M. Moreno Julia R. Ferreira Raphael E. Szawka Ana C. Campideli-Santana Jullia Teles Luciano S. A. Capettini Flaviano S. Martins Maria A. Gomes |
| author_facet | Vivian Moura-Oliveira Fabrício M. S. Oliveira Olga L. M. Moreno Julia R. Ferreira Raphael E. Szawka Ana C. Campideli-Santana Jullia Teles Luciano S. A. Capettini Flaviano S. Martins Maria A. Gomes |
| author_sort | Vivian Moura-Oliveira |
| collection | DOAJ |
| description | Amebiasis is a globally prevalent infection that can lead to fatal outcomes if not adequately treated. Conventional treatment with imidazoles often fails due to side effects and resistance, emphasizing the need for alternative therapies. The probiotic <i>Escherichia coli</i> Nissle 1917 (EcN) has shown potential in combating intestinal pathogens. This study aimed to evaluate the amebicidal activity of EcN in vitro and its effect on the production of reactive oxygen species (ROS). Trophozoites of <i>Entamoeba histolytica</i> (2.5 × 10⁴ cells/mL) were cultured in 96-well plates and exposed to varying concentrations of EcN (10<sup>2</sup>–10<sup>9</sup> cells/mL). Plates were incubated at 36 °C for 6, 12, and 18 h, after which trophozoite viability was assessed. Intracellular ROS production, including superoxide and hydrogen peroxide, was measured using fluorescent probes. The highest efficacy was observed after 18 h at a CFU concentration of 10<sup>9</sup> cells/mL. Increased ROS production at all probiotic concentrations suggested a role in EcN’s amebicidal mechanism. Morphological changes in trophozoites, such as rounding, vacuolization, and size reduction, were noted after EcN exposure, indicating growth inhibition. These findings suggest EcN induces structural and morphological changes in <i>E. histolytica</i>, inhibiting its growth in vitro. The findings suggest the potential efficacy of EcN; however, definitive confirmation requires data from human clinical trials. |
| format | Article |
| id | doaj-art-c73891d800b943c7afcc440537f294b7 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-c73891d800b943c7afcc440537f294b72025-08-20T03:13:58ZengMDPI AGMicroorganisms2076-26072025-04-0113482810.3390/microorganisms13040828Amebicidal Activity of <i>Escherichia coli</i> Nissle 1917 Against <i>Entamoeba histolytica</i>Vivian Moura-Oliveira0Fabrício M. S. Oliveira1Olga L. M. Moreno2Julia R. Ferreira3Raphael E. Szawka4Ana C. Campideli-Santana5Jullia Teles6Luciano S. A. Capettini7Flaviano S. Martins8Maria A. Gomes9Department of Parasitology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilTranslational Type 2 Immunity Laboratory, Microbiology, Immunology and Tropical Medicine Department, George Washington School of Medicine and Health Sciences, Washington, DC 20052, USADepartment of Pharmacology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilDepartment of Parasitology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilDepartment of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilDepartment of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilDepartment of Parasitology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilDepartment of Pharmacology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilDepartment of Microbiology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilDepartment of Parasitology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilAmebiasis is a globally prevalent infection that can lead to fatal outcomes if not adequately treated. Conventional treatment with imidazoles often fails due to side effects and resistance, emphasizing the need for alternative therapies. The probiotic <i>Escherichia coli</i> Nissle 1917 (EcN) has shown potential in combating intestinal pathogens. This study aimed to evaluate the amebicidal activity of EcN in vitro and its effect on the production of reactive oxygen species (ROS). Trophozoites of <i>Entamoeba histolytica</i> (2.5 × 10⁴ cells/mL) were cultured in 96-well plates and exposed to varying concentrations of EcN (10<sup>2</sup>–10<sup>9</sup> cells/mL). Plates were incubated at 36 °C for 6, 12, and 18 h, after which trophozoite viability was assessed. Intracellular ROS production, including superoxide and hydrogen peroxide, was measured using fluorescent probes. The highest efficacy was observed after 18 h at a CFU concentration of 10<sup>9</sup> cells/mL. Increased ROS production at all probiotic concentrations suggested a role in EcN’s amebicidal mechanism. Morphological changes in trophozoites, such as rounding, vacuolization, and size reduction, were noted after EcN exposure, indicating growth inhibition. These findings suggest EcN induces structural and morphological changes in <i>E. histolytica</i>, inhibiting its growth in vitro. The findings suggest the potential efficacy of EcN; however, definitive confirmation requires data from human clinical trials.https://www.mdpi.com/2076-2607/13/4/828<i>Entamoeba histolytica</i><i>Escherichia coli</i>Nissleprobioticreactive oxygen species |
| spellingShingle | Vivian Moura-Oliveira Fabrício M. S. Oliveira Olga L. M. Moreno Julia R. Ferreira Raphael E. Szawka Ana C. Campideli-Santana Jullia Teles Luciano S. A. Capettini Flaviano S. Martins Maria A. Gomes Amebicidal Activity of <i>Escherichia coli</i> Nissle 1917 Against <i>Entamoeba histolytica</i> Microorganisms <i>Entamoeba histolytica</i> <i>Escherichia coli</i> Nissle probiotic reactive oxygen species |
| title | Amebicidal Activity of <i>Escherichia coli</i> Nissle 1917 Against <i>Entamoeba histolytica</i> |
| title_full | Amebicidal Activity of <i>Escherichia coli</i> Nissle 1917 Against <i>Entamoeba histolytica</i> |
| title_fullStr | Amebicidal Activity of <i>Escherichia coli</i> Nissle 1917 Against <i>Entamoeba histolytica</i> |
| title_full_unstemmed | Amebicidal Activity of <i>Escherichia coli</i> Nissle 1917 Against <i>Entamoeba histolytica</i> |
| title_short | Amebicidal Activity of <i>Escherichia coli</i> Nissle 1917 Against <i>Entamoeba histolytica</i> |
| title_sort | amebicidal activity of i escherichia coli i nissle 1917 against i entamoeba histolytica i |
| topic | <i>Entamoeba histolytica</i> <i>Escherichia coli</i> Nissle probiotic reactive oxygen species |
| url | https://www.mdpi.com/2076-2607/13/4/828 |
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