Investigating the Prevalence of Enterotoxin and Antibiotic Resistance in Methicillin‐Resistant Staphylococcus aureus (MRSA) Isolated From Meat and Edible Viscera of Broiler Chickens
ABSTRACT Background The responsible for staph infection is methicillin‐resistant Staphylococcus aureus (MRSA) which has a long and difficult treatment process due to resistance to this type of antibiotic. This study is designed to investigate the distribution and frequency of antibiotic‐resistant ge...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-07-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.70413 |
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| Summary: | ABSTRACT Background The responsible for staph infection is methicillin‐resistant Staphylococcus aureus (MRSA) which has a long and difficult treatment process due to resistance to this type of antibiotic. This study is designed to investigate the distribution and frequency of antibiotic‐resistant genes and MRSA enterotoxins isolated from the meat and edible viscera of broiler chickens, which are responsible for pathogenicity in humans. Materials and Methods A total of 523 meat and edible viscera of broiler chicken collected from farms in Shahrekord, Iran. The antibiogram test of 142 MRSA isolates was performed by Kirby‐Bauer diffusion disc. Sensitivity or resistance of MRSA was tested on 13 different antibiotics. DNA extracted from MRSA was screened by PCR technique for the presence of antibiotic‐resistant and enterotoxin genes. Results Staphylococcus aureus (S. aureus) isolated with frequency of 51.05% (267 of 523). The presence of mecA gene in S. aureus was examined to detect the MRSA. The most antibiotic‐resistance responsible genes and the pathogenic enterotoxin genes were identified. MRSA was identified by positive amplification of mecA in 53.18% (142 of 267) of S. aureus isolates. S. aureus antimicrobial resistance was most frequently noted against tetracycline (94.37%), ampicillin (88.73%) and penicillin (71.83%). Out of 51 examined isolates, 47 isolates exhibited the sea (92.15%), and 7 isolates exhibited the sej (13.72%). Conclusion The results indicated high prevalence of MRSA in broilers, which is very worrying issue. In addition, in the present study, it was observed that due to the increase in the use of antibiotics in poultry farming, bacteria resistant to methicillin and other antibiotics have a high prevalence. Now, with the knowledge that the consumption of broiler chicken is very high all over the world and with the increasing trend of antibiotic consumption, this issue has become a concern at the global health level. The presence of antibiotic‐resistance and enterotoxigenic genes in MRSA bacteria is a critical threat to human nutrition, making consuming contaminated meat and edible viscera of broiler chickens unsafe. |
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| ISSN: | 2053-1095 |