Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome
Background: Aspergillus coinfection in patients with severe fever with thrombocytopenia syndrome (SFTS), is a serious clinical challenge associated with increased mortality. Understanding the factors contributing to this co-infection is crucial for improving patient outcomes. This study aimed to rev...
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Elsevier
2025-09-01
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| Series: | Journal of Infection and Public Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034125001868 |
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| author | Meng-Yu Liu Sheng-Fu He Yu-Yao Li Jiao-Jiao Shen Jia-Jia Li Ya-Sheng Li Yan-Yan Liu Ting Wu Jia-Bin Li Li-Fen Hu |
| author_facet | Meng-Yu Liu Sheng-Fu He Yu-Yao Li Jiao-Jiao Shen Jia-Jia Li Ya-Sheng Li Yan-Yan Liu Ting Wu Jia-Bin Li Li-Fen Hu |
| author_sort | Meng-Yu Liu |
| collection | DOAJ |
| description | Background: Aspergillus coinfection in patients with severe fever with thrombocytopenia syndrome (SFTS), is a serious clinical challenge associated with increased mortality. Understanding the factors contributing to this co-infection is crucial for improving patient outcomes. This study aimed to reveal the role of the intestinal mycobiome in SFTS severity and the risk of Aspergillus coinfection, with the goal of identifying potential therapeutic targets. Methods: Fecal samples were collected from 80 patients both during their hospitalization and post-discharge. Internal transcribed spacer (ITS) amplicon sequencing and fungal profiling of intestine were performed. R statistical software (version 3.5.1) was used for data processing and analysis. Results: The intestinal mycobiomes of SFTS patients showed strong alterations characterized by increased Aspergillus species, and a highly heterogeneous mycobiome configuration compared to healthy controls. The Aspergillus had a positive correlation with coinfection of invasive pulmonary aspergillosis (IPA) and disease severity of SFTS (p < 0.001), whereas Saccharomycetales and Candida were more abundant in SFTS patients without IPA (p < 0.001). In SFTS patients with IPA, A. subversicolor, A. flavus and A. penicillioides were the three most common fungal species. Longitudinal dynamic detection revealed that patients who experienced significant fluctuations in their intestinal mycobiome tended to have more severe illness. After recovering, the gut mycobiome of patients can recover and stabilize within a month. Conclusion: The research highlighted enrichment of intestinal Aspergillus was conducive to IPA and disease severity in SFTS patients. Monitoring the gut mycobiome could potentially be used as a biomarker to assess disease severity of SFTS. |
| format | Article |
| id | doaj-art-c7275f394dbe46c3ac64e88d96c77c8a |
| institution | Kabale University |
| issn | 1876-0341 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj-art-c7275f394dbe46c3ac64e88d96c77c8a2025-08-20T03:42:34ZengElsevierJournal of Infection and Public Health1876-03412025-09-0118910283710.1016/j.jiph.2025.102837Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndromeMeng-Yu Liu0Sheng-Fu He1Yu-Yao Li2Jiao-Jiao Shen3Jia-Jia Li4Ya-Sheng Li5Yan-Yan Liu6Ting Wu7Jia-Bin Li8Li-Fen Hu9Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, ChinaAnhui Province Key Laboratory of Infectious Diseases, Anhui Medical University, Hefei, China; Institute of Bacterial Resistance, Anhui Medical University, Hefei, China; Department of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, ChinaDepartment of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, ChinaDepartment of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, ChinaAnhui Province Key Laboratory of Infectious Diseases, Anhui Medical University, Hefei, ChinaAnhui Province Key Laboratory of Infectious Diseases, Anhui Medical University, Hefei, China; Institute of Bacterial Resistance, Anhui Medical University, Hefei, ChinaAnhui Province Key Laboratory of Infectious Diseases, Anhui Medical University, Hefei, China; Institute of Bacterial Resistance, Anhui Medical University, Hefei, ChinaDepartment of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China; Anhui Province Key Laboratory of Infectious Diseases, Anhui Medical University, Hefei, China; Institute of Bacterial Resistance, Anhui Medical University, Hefei, China; Correspondence to: Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Anhui Province Key Laboratory of Infectious Diseases, Jixi Road 218, Hefei, Anhui, ChinaDepartment of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China; Anhui Province Key Laboratory of Infectious Diseases, Anhui Medical University, Hefei, China; Institute of Bacterial Resistance, Anhui Medical University, Hefei, China; Correspondence to: Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Anhui Province Key Laboratory of Infectious Diseases, Jixi Road 218, Hefei, Anhui, ChinaDepartment of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China; Anhui Province Key Laboratory of Infectious Diseases, Anhui Medical University, Hefei, China; Institute of Bacterial Resistance, Anhui Medical University, Hefei, China; Correspondence to: Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Anhui Province Key Laboratory of Infectious Diseases, Jixi Road 218, Hefei, Anhui, ChinaBackground: Aspergillus coinfection in patients with severe fever with thrombocytopenia syndrome (SFTS), is a serious clinical challenge associated with increased mortality. Understanding the factors contributing to this co-infection is crucial for improving patient outcomes. This study aimed to reveal the role of the intestinal mycobiome in SFTS severity and the risk of Aspergillus coinfection, with the goal of identifying potential therapeutic targets. Methods: Fecal samples were collected from 80 patients both during their hospitalization and post-discharge. Internal transcribed spacer (ITS) amplicon sequencing and fungal profiling of intestine were performed. R statistical software (version 3.5.1) was used for data processing and analysis. Results: The intestinal mycobiomes of SFTS patients showed strong alterations characterized by increased Aspergillus species, and a highly heterogeneous mycobiome configuration compared to healthy controls. The Aspergillus had a positive correlation with coinfection of invasive pulmonary aspergillosis (IPA) and disease severity of SFTS (p < 0.001), whereas Saccharomycetales and Candida were more abundant in SFTS patients without IPA (p < 0.001). In SFTS patients with IPA, A. subversicolor, A. flavus and A. penicillioides were the three most common fungal species. Longitudinal dynamic detection revealed that patients who experienced significant fluctuations in their intestinal mycobiome tended to have more severe illness. After recovering, the gut mycobiome of patients can recover and stabilize within a month. Conclusion: The research highlighted enrichment of intestinal Aspergillus was conducive to IPA and disease severity in SFTS patients. Monitoring the gut mycobiome could potentially be used as a biomarker to assess disease severity of SFTS.http://www.sciencedirect.com/science/article/pii/S1876034125001868Severe fever with thrombocytopenia syndromeIntestineMycobiomeInvasive pulmonary aspergillosisDabie bandavirus |
| spellingShingle | Meng-Yu Liu Sheng-Fu He Yu-Yao Li Jiao-Jiao Shen Jia-Jia Li Ya-Sheng Li Yan-Yan Liu Ting Wu Jia-Bin Li Li-Fen Hu Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome Journal of Infection and Public Health Severe fever with thrombocytopenia syndrome Intestine Mycobiome Invasive pulmonary aspergillosis Dabie bandavirus |
| title | Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome |
| title_full | Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome |
| title_fullStr | Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome |
| title_full_unstemmed | Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome |
| title_short | Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome |
| title_sort | alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome |
| topic | Severe fever with thrombocytopenia syndrome Intestine Mycobiome Invasive pulmonary aspergillosis Dabie bandavirus |
| url | http://www.sciencedirect.com/science/article/pii/S1876034125001868 |
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