Atlas of Interactions Between Decoration Proteins and Major Capsid Proteins of Coliphage N4
Coliphage N4 is a representative species of the <i>Schitoviridae</i> family of bacteriophages. Originally structurally studied in 2008, the capsid structure was solved to 14 Å to reveal an interesting arrangement of Ig-like decoration proteins across the surface of the capsid. Herein, we...
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2024-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/17/1/19 |
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| author | Klem McJarrow-Keller Alice-Roza Eruera Alexander J. M. Crowe Rosheny Kumaran Jaekyung Hyun Mihnea Bostina |
| author_facet | Klem McJarrow-Keller Alice-Roza Eruera Alexander J. M. Crowe Rosheny Kumaran Jaekyung Hyun Mihnea Bostina |
| author_sort | Klem McJarrow-Keller |
| collection | DOAJ |
| description | Coliphage N4 is a representative species of the <i>Schitoviridae</i> family of bacteriophages. Originally structurally studied in 2008, the capsid structure was solved to 14 Å to reveal an interesting arrangement of Ig-like decoration proteins across the surface of the capsid. Herein, we present a high-resolution N4 structure, reporting a 2.45 Å map of the capsid obtained via single particle cryogenic-electron microscopy. Structural analysis of the major capsid proteins (MCPs) and decoration proteins (gp56 and gp17) of phage N4 reveals a pattern of interactions across the capsid that are mediated by structurally homologous domains of gp17. In this study, an analysis of the complex interface contacts allows us to confirm that the gp17 Ig-like decoration proteins of N4 are likely employed by the virus to increase the capsid’s structural integrity. |
| format | Article |
| id | doaj-art-c7235c10af3849b7b1462d872107958b |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-c7235c10af3849b7b1462d872107958b2025-01-24T13:52:16ZengMDPI AGViruses1999-49152024-12-011711910.3390/v17010019Atlas of Interactions Between Decoration Proteins and Major Capsid Proteins of Coliphage N4Klem McJarrow-Keller0Alice-Roza Eruera1Alexander J. M. Crowe2Rosheny Kumaran3Jaekyung Hyun4Mihnea Bostina5Department of Microbiology and Immunology, University of Otago, Dunedin 9016, New ZealandDepartment of Microbiology and Immunology, University of Otago, Dunedin 9016, New ZealandDepartment of Microbiology and Immunology, University of Otago, Dunedin 9016, New ZealandDepartment of Microbiology and Immunology, University of Otago, Dunedin 9016, New ZealandSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of KoreaDepartment of Microbiology and Immunology, University of Otago, Dunedin 9016, New ZealandColiphage N4 is a representative species of the <i>Schitoviridae</i> family of bacteriophages. Originally structurally studied in 2008, the capsid structure was solved to 14 Å to reveal an interesting arrangement of Ig-like decoration proteins across the surface of the capsid. Herein, we present a high-resolution N4 structure, reporting a 2.45 Å map of the capsid obtained via single particle cryogenic-electron microscopy. Structural analysis of the major capsid proteins (MCPs) and decoration proteins (gp56 and gp17) of phage N4 reveals a pattern of interactions across the capsid that are mediated by structurally homologous domains of gp17. In this study, an analysis of the complex interface contacts allows us to confirm that the gp17 Ig-like decoration proteins of N4 are likely employed by the virus to increase the capsid’s structural integrity.https://www.mdpi.com/1999-4915/17/1/19cryo-electron microscopybacteriophage N4capsid decoration proteinIg-like decoration protein |
| spellingShingle | Klem McJarrow-Keller Alice-Roza Eruera Alexander J. M. Crowe Rosheny Kumaran Jaekyung Hyun Mihnea Bostina Atlas of Interactions Between Decoration Proteins and Major Capsid Proteins of Coliphage N4 Viruses cryo-electron microscopy bacteriophage N4 capsid decoration protein Ig-like decoration protein |
| title | Atlas of Interactions Between Decoration Proteins and Major Capsid Proteins of Coliphage N4 |
| title_full | Atlas of Interactions Between Decoration Proteins and Major Capsid Proteins of Coliphage N4 |
| title_fullStr | Atlas of Interactions Between Decoration Proteins and Major Capsid Proteins of Coliphage N4 |
| title_full_unstemmed | Atlas of Interactions Between Decoration Proteins and Major Capsid Proteins of Coliphage N4 |
| title_short | Atlas of Interactions Between Decoration Proteins and Major Capsid Proteins of Coliphage N4 |
| title_sort | atlas of interactions between decoration proteins and major capsid proteins of coliphage n4 |
| topic | cryo-electron microscopy bacteriophage N4 capsid decoration protein Ig-like decoration protein |
| url | https://www.mdpi.com/1999-4915/17/1/19 |
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