Kinetic Modeling and Biological Activities of <i>Rosa canina</i> L. Pseudo-Fruit Extracts Obtained via Enzyme-Assisted Extraction
This study investigates the enzyme-assisted extraction (EAE) of bioactive compounds from the pseudo-fruit of the wild rose (<i>Rosa canina</i> L.), also known as rosehip, using a commercial cellulolytic enzyme preparation, Cellic<sup>®</sup> CTec3 HS. The effects of extractio...
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MDPI AG
2025-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/5/558 |
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| author | Zafeiria Lemoni Styliani Kalantzi Theopisti Lymperopoulou Andromachi Tzani Georgios Stavropoulos Anastasia Detsi Diomi Mamma |
| author_facet | Zafeiria Lemoni Styliani Kalantzi Theopisti Lymperopoulou Andromachi Tzani Georgios Stavropoulos Anastasia Detsi Diomi Mamma |
| author_sort | Zafeiria Lemoni |
| collection | DOAJ |
| description | This study investigates the enzyme-assisted extraction (EAE) of bioactive compounds from the pseudo-fruit of the wild rose (<i>Rosa canina</i> L.), also known as rosehip, using a commercial cellulolytic enzyme preparation, Cellic<sup>®</sup> CTec3 HS. The effects of extraction time, solid to liquid ratio, and enzyme loading on total phenolic content (TPC) and total flavonoid content (TFC) were evaluated. The highest yields of TPC (168.3 ± 1.1 GAE/g DM) and TFC (72.3 ± 0.8 mg CAE/g DM) were obtained at 360 min, using 1% (<i>v</i>/<i>v</i>) enzyme loading and a 6% (<i>w</i>/<i>v</i>) solid to liquid ratio. Kinetic modeling of the extraction process was performed using first-order, second-order, Peleg’s, and power law models. The power law model best described the extraction dynamics. The obtained extracts were assessed for their biological activities including antioxidant, antimicrobial, anti-aging, and anti-diabetic properties. The extract obtained under optimal extraction conditions exhibited potent tyrosinase inhibition (80%) and moderate to low inhibition of α-glucosidase (15%) and α-amylase (20%) activities. The IC<sub>50</sub> for DPPH radical scavenging was 0.44 μL extract/mL while the extract exhibited significant antibacterial activity against <i>Escherichia coli</i> growth (79% inhibition). These findings suggest that the extract, obtained through EAE, has promising biological properties with potential applications in the food, pharmaceutical, and cosmetic industries. |
| format | Article |
| id | doaj-art-c71b5f7c531947b7bd2d9664ba0d49f9 |
| institution | DOAJ |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-c71b5f7c531947b7bd2d9664ba0d49f92025-08-20T03:14:29ZengMDPI AGAntioxidants2076-39212025-05-0114555810.3390/antiox14050558Kinetic Modeling and Biological Activities of <i>Rosa canina</i> L. Pseudo-Fruit Extracts Obtained via Enzyme-Assisted ExtractionZafeiria Lemoni0Styliani Kalantzi1Theopisti Lymperopoulou2Andromachi Tzani3Georgios Stavropoulos4Anastasia Detsi5Diomi Mamma6Biotechnology Laboratory, School of Chemical Engineering, Zografou Campus, National Technical University of Athens, 9 Iroon Polytechniou Str, 15780 Athens, GreeceBiotechnology Laboratory, School of Chemical Engineering, Zografou Campus, National Technical University of Athens, 9 Iroon Polytechniou Str, 15780 Athens, GreeceProducts and Operations Quality Control Laboratory, School of Chemical Engineering, Zografou Campus, National Technical University of Athens, 15780 Athens, GreeceLaboratory of Organic Chemistry, School of Chemical Engineering, Zografou Campus, National Technical University of Athens, 15780 Athens, GreeceKorres S.A.—Natural Products, 57th km Athens-Lamia Road, 32011 Oinofyta Viotia, GreeceLaboratory of Organic Chemistry, School of Chemical Engineering, Zografou Campus, National Technical University of Athens, 15780 Athens, GreeceBiotechnology Laboratory, School of Chemical Engineering, Zografou Campus, National Technical University of Athens, 9 Iroon Polytechniou Str, 15780 Athens, GreeceThis study investigates the enzyme-assisted extraction (EAE) of bioactive compounds from the pseudo-fruit of the wild rose (<i>Rosa canina</i> L.), also known as rosehip, using a commercial cellulolytic enzyme preparation, Cellic<sup>®</sup> CTec3 HS. The effects of extraction time, solid to liquid ratio, and enzyme loading on total phenolic content (TPC) and total flavonoid content (TFC) were evaluated. The highest yields of TPC (168.3 ± 1.1 GAE/g DM) and TFC (72.3 ± 0.8 mg CAE/g DM) were obtained at 360 min, using 1% (<i>v</i>/<i>v</i>) enzyme loading and a 6% (<i>w</i>/<i>v</i>) solid to liquid ratio. Kinetic modeling of the extraction process was performed using first-order, second-order, Peleg’s, and power law models. The power law model best described the extraction dynamics. The obtained extracts were assessed for their biological activities including antioxidant, antimicrobial, anti-aging, and anti-diabetic properties. The extract obtained under optimal extraction conditions exhibited potent tyrosinase inhibition (80%) and moderate to low inhibition of α-glucosidase (15%) and α-amylase (20%) activities. The IC<sub>50</sub> for DPPH radical scavenging was 0.44 μL extract/mL while the extract exhibited significant antibacterial activity against <i>Escherichia coli</i> growth (79% inhibition). These findings suggest that the extract, obtained through EAE, has promising biological properties with potential applications in the food, pharmaceutical, and cosmetic industries.https://www.mdpi.com/2076-3921/14/5/558enzyme-assisted extraction (EAE)cellulolytic enzymepseudo-fruit of <i>Rosa canina</i> L.total phenolic content (TPC)total flavonoid content (TFC)kinetic models |
| spellingShingle | Zafeiria Lemoni Styliani Kalantzi Theopisti Lymperopoulou Andromachi Tzani Georgios Stavropoulos Anastasia Detsi Diomi Mamma Kinetic Modeling and Biological Activities of <i>Rosa canina</i> L. Pseudo-Fruit Extracts Obtained via Enzyme-Assisted Extraction Antioxidants enzyme-assisted extraction (EAE) cellulolytic enzyme pseudo-fruit of <i>Rosa canina</i> L. total phenolic content (TPC) total flavonoid content (TFC) kinetic models |
| title | Kinetic Modeling and Biological Activities of <i>Rosa canina</i> L. Pseudo-Fruit Extracts Obtained via Enzyme-Assisted Extraction |
| title_full | Kinetic Modeling and Biological Activities of <i>Rosa canina</i> L. Pseudo-Fruit Extracts Obtained via Enzyme-Assisted Extraction |
| title_fullStr | Kinetic Modeling and Biological Activities of <i>Rosa canina</i> L. Pseudo-Fruit Extracts Obtained via Enzyme-Assisted Extraction |
| title_full_unstemmed | Kinetic Modeling and Biological Activities of <i>Rosa canina</i> L. Pseudo-Fruit Extracts Obtained via Enzyme-Assisted Extraction |
| title_short | Kinetic Modeling and Biological Activities of <i>Rosa canina</i> L. Pseudo-Fruit Extracts Obtained via Enzyme-Assisted Extraction |
| title_sort | kinetic modeling and biological activities of i rosa canina i l pseudo fruit extracts obtained via enzyme assisted extraction |
| topic | enzyme-assisted extraction (EAE) cellulolytic enzyme pseudo-fruit of <i>Rosa canina</i> L. total phenolic content (TPC) total flavonoid content (TFC) kinetic models |
| url | https://www.mdpi.com/2076-3921/14/5/558 |
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