Effects of Hydroxyapatite Nanoparticles on Devitrification crystallization of Different kinds of Cryoprotectants

To study the effects of Hydroxyapatite (HA) nanoparticles on devitrification of different cryoprotectant solutions, the crystallization of Glycerol(60%,w/w) and PEG-600(50%,w/w) solutions with HA nanoparticles of different particle size(20nm、40nm、60nm)and concentrations(0.1%、0.5%)were observed under...

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Bibliographic Details
Main Authors: Lv Fukou, Liu Baolin, Li Weijie
Format: Article
Language:zho
Published: Journal of Refrigeration Magazines Agency Co., Ltd. 2013-01-01
Series:Zhileng xuebao
Subjects:
Online Access:http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2013.04.018
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Summary:To study the effects of Hydroxyapatite (HA) nanoparticles on devitrification of different cryoprotectant solutions, the crystallization of Glycerol(60%,w/w) and PEG-600(50%,w/w) solutions with HA nanoparticles of different particle size(20nm、40nm、60nm)and concentrations(0.1%、0.5%)were observed under a cryomicroscope. Experimental results showed that the morphology of Glycerol and PEG-600 solutions is dendritic and spheric respectively, and both structures are not changed by adding nanoparticles. The ice fraction of Glycerol solution containing 0.5% HA with the particle size of 60nm is 1/7 of that of Glycerol solution without nanoparticles. The ice fraction of PEG-600 solution increased drastically between -64℃ and -52℃, and the ice fraction of PEG-600 solution containing 0.5% HA with the particle size of 40nm increased by 90%. Ice growth rate has two maximum normally, however, there is only one maximum in Glycerol solution containing 0.5% HA with the particle size of 40nm, PEG-600 solution with the particle size of 60nm, and PEG-600 solution without nanoparticles.
ISSN:0253-4339