Effects of 0–12% soy proteins (four texturized and one isolate) on a lean hybrid meat system: cooking loss, texture, dynamic rheology, microstructure, and T2 NMR

Meat and plant hybrid products have recently emerged as part of the global plant-forward movement. Soy proteins have been used at low levels (2–3 %), in meat products since the 1960s, mainly to enhance yield and sensory characteristics. This study assessed the structure-function relationship in prod...

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Main Authors: Weilun Lin, Shai Barbut
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225000575
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author Weilun Lin
Shai Barbut
author_facet Weilun Lin
Shai Barbut
author_sort Weilun Lin
collection DOAJ
description Meat and plant hybrid products have recently emerged as part of the global plant-forward movement. Soy proteins have been used at low levels (2–3 %), in meat products since the 1960s, mainly to enhance yield and sensory characteristics. This study assessed the structure-function relationship in products containing 0–12 % soy protein isolate (SPI) and four texturized soy proteins (TSPs: TA, TB, TC, and TD). Soy proteins significantly reduced cooking loss and increased hardness compared to the control (CL, no soy), with these effects intensifying as inclusion levels increased. At 12 %, TD resulted in lower hardness than the other soy proteins. Dynamic rheology revealed that at 6% inclusion, SPI increased the final storage modulus (G') compared to the CL, whereas TA, TB, and TC decreased it. The TD treatment exhibited a final G' similar to CL. Micrographs showed that 12 % TB (smallest texturized soy particles) caused discontinuity in the meat matrix, while TD (largest particles) confined meat components within its structure. T2 NMR profiles revealed that all soy proteins restricted the water mobility of the meat batters, with TSPs showing a more pronounced effect than SPI. The relative order of T21 values of cooked meat batters aligns with the cooking loss results. Overall, TSPs showed superior water binding to SPI. In this study, the larger size of TSPs likely had a favorable effect on their binding to the meat matrix at the high inclusion level (12 %). These findings provide insights into the selection of soy proteins for hybrid meat production.
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spelling doaj-art-c71511694acd4e319903c017badc268e2025-08-20T02:07:02ZengElsevierApplied Food Research2772-50222025-06-015110074710.1016/j.afres.2025.100747Effects of 0–12% soy proteins (four texturized and one isolate) on a lean hybrid meat system: cooking loss, texture, dynamic rheology, microstructure, and T2 NMRWeilun Lin0Shai Barbut1Dept. of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1Dept. of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1; Adaptation Physiology Group, Wageningen University & Research, Netherlands; Corresponding author.Meat and plant hybrid products have recently emerged as part of the global plant-forward movement. Soy proteins have been used at low levels (2–3 %), in meat products since the 1960s, mainly to enhance yield and sensory characteristics. This study assessed the structure-function relationship in products containing 0–12 % soy protein isolate (SPI) and four texturized soy proteins (TSPs: TA, TB, TC, and TD). Soy proteins significantly reduced cooking loss and increased hardness compared to the control (CL, no soy), with these effects intensifying as inclusion levels increased. At 12 %, TD resulted in lower hardness than the other soy proteins. Dynamic rheology revealed that at 6% inclusion, SPI increased the final storage modulus (G') compared to the CL, whereas TA, TB, and TC decreased it. The TD treatment exhibited a final G' similar to CL. Micrographs showed that 12 % TB (smallest texturized soy particles) caused discontinuity in the meat matrix, while TD (largest particles) confined meat components within its structure. T2 NMR profiles revealed that all soy proteins restricted the water mobility of the meat batters, with TSPs showing a more pronounced effect than SPI. The relative order of T21 values of cooked meat batters aligns with the cooking loss results. Overall, TSPs showed superior water binding to SPI. In this study, the larger size of TSPs likely had a favorable effect on their binding to the meat matrix at the high inclusion level (12 %). These findings provide insights into the selection of soy proteins for hybrid meat production.http://www.sciencedirect.com/science/article/pii/S2772502225000575Hybrid meatTextureMicrostructureNuclear magnetic resonanceSoy proteinsRheology
spellingShingle Weilun Lin
Shai Barbut
Effects of 0–12% soy proteins (four texturized and one isolate) on a lean hybrid meat system: cooking loss, texture, dynamic rheology, microstructure, and T2 NMR
Applied Food Research
Hybrid meat
Texture
Microstructure
Nuclear magnetic resonance
Soy proteins
Rheology
title Effects of 0–12% soy proteins (four texturized and one isolate) on a lean hybrid meat system: cooking loss, texture, dynamic rheology, microstructure, and T2 NMR
title_full Effects of 0–12% soy proteins (four texturized and one isolate) on a lean hybrid meat system: cooking loss, texture, dynamic rheology, microstructure, and T2 NMR
title_fullStr Effects of 0–12% soy proteins (four texturized and one isolate) on a lean hybrid meat system: cooking loss, texture, dynamic rheology, microstructure, and T2 NMR
title_full_unstemmed Effects of 0–12% soy proteins (four texturized and one isolate) on a lean hybrid meat system: cooking loss, texture, dynamic rheology, microstructure, and T2 NMR
title_short Effects of 0–12% soy proteins (four texturized and one isolate) on a lean hybrid meat system: cooking loss, texture, dynamic rheology, microstructure, and T2 NMR
title_sort effects of 0 12 soy proteins four texturized and one isolate on a lean hybrid meat system cooking loss texture dynamic rheology microstructure and t2 nmr
topic Hybrid meat
Texture
Microstructure
Nuclear magnetic resonance
Soy proteins
Rheology
url http://www.sciencedirect.com/science/article/pii/S2772502225000575
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