Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea
Objectives. The aim of this study was to evaluate the clinical application of three methods for detecting Clostridium difficile in fecal samples. Methods. One hundred and fifty fecal specimens were collected and tested for C. difficile using three methods: (1) the toxigenic culture (TC); (2) the VID...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/8753284 |
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| author | Yanyan Xiao Yong Liu Xiaosong Qin |
| author_facet | Yanyan Xiao Yong Liu Xiaosong Qin |
| author_sort | Yanyan Xiao |
| collection | DOAJ |
| description | Objectives. The aim of this study was to evaluate the clinical application of three methods for detecting Clostridium difficile in fecal samples. Methods. One hundred and fifty fecal specimens were collected and tested for C. difficile using three methods: (1) the toxigenic culture (TC); (2) the VIDAS enzyme immunoassay (EIA): the VIDAS glutamate dehydrogenase (GDH) assay and toxin A/B assay were used to detect GDH antigen and A/B toxin; and (3) the GeneXpert PCR assay. The toxigenic culture was used as a reference to evaluate the performance of the VIDAS EIA and the GeneXpert PCR assay. Results. Of 150 specimens, 26 carried both A and B toxin genes, and none of the samples were positive for the binary toxin gene. Toxin-producing C. difficile was found in 17.3% (26/150) of the samples. Thirty-seven GDH-positive samples were detected using the VIDAS GDH assay, and 15 toxin-positive samples were detected using the VIDAS toxin A/B assay. The GeneXpert PCR assay was used to detect C. difficile in 79 specimens simultaneously, and a total of 18 positive specimens were detected. Conclusion. The VIDAS GDH assay is useful for initial screening of C. difficile. The GeneXpert PCR assay is a simple and quick method. |
| format | Article |
| id | doaj-art-c70f8bbc969d425382f0354435ec1128 |
| institution | OA Journals |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-c70f8bbc969d425382f0354435ec11282025-08-20T02:19:45ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932020-01-01202010.1155/2020/87532848753284Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with DiarrhoeaYanyan Xiao0Yong Liu1Xiaosong Qin2Department of Clinical Laboratory, Shengjing Hospital of China Medical University, Shenyang, ChinaDepartment of Clinical Laboratory, Shengjing Hospital of China Medical University, Shenyang, ChinaDepartment of Clinical Laboratory, Shengjing Hospital of China Medical University, Shenyang, ChinaObjectives. The aim of this study was to evaluate the clinical application of three methods for detecting Clostridium difficile in fecal samples. Methods. One hundred and fifty fecal specimens were collected and tested for C. difficile using three methods: (1) the toxigenic culture (TC); (2) the VIDAS enzyme immunoassay (EIA): the VIDAS glutamate dehydrogenase (GDH) assay and toxin A/B assay were used to detect GDH antigen and A/B toxin; and (3) the GeneXpert PCR assay. The toxigenic culture was used as a reference to evaluate the performance of the VIDAS EIA and the GeneXpert PCR assay. Results. Of 150 specimens, 26 carried both A and B toxin genes, and none of the samples were positive for the binary toxin gene. Toxin-producing C. difficile was found in 17.3% (26/150) of the samples. Thirty-seven GDH-positive samples were detected using the VIDAS GDH assay, and 15 toxin-positive samples were detected using the VIDAS toxin A/B assay. The GeneXpert PCR assay was used to detect C. difficile in 79 specimens simultaneously, and a total of 18 positive specimens were detected. Conclusion. The VIDAS GDH assay is useful for initial screening of C. difficile. The GeneXpert PCR assay is a simple and quick method.http://dx.doi.org/10.1155/2020/8753284 |
| spellingShingle | Yanyan Xiao Yong Liu Xiaosong Qin Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea |
| title_full | Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea |
| title_fullStr | Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea |
| title_full_unstemmed | Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea |
| title_short | Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea |
| title_sort | comparative study of clostridium difficile clinical detection methods in patients with diarrhoea |
| url | http://dx.doi.org/10.1155/2020/8753284 |
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