The Gut Microbiota of the Greater Horseshoe Bat Confers Rapidly Corresponding Immune Cells in Mice

Background: Emerging infectious diseases threaten human and animal health, with most pathogens originating from wildlife. Bats are natural hosts for many infectious agents. Previous studies have demonstrated that changes in some specific genes in bats may contribute to resistance to viral infections...

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Main Authors: Shan Luo, Xinlei Huang, Siyu Chen, Junyi Li, Hui Wu, Yuhua He, Lei Zhou, Boyu Liu, Jiang Feng
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/5/685
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author Shan Luo
Xinlei Huang
Siyu Chen
Junyi Li
Hui Wu
Yuhua He
Lei Zhou
Boyu Liu
Jiang Feng
author_facet Shan Luo
Xinlei Huang
Siyu Chen
Junyi Li
Hui Wu
Yuhua He
Lei Zhou
Boyu Liu
Jiang Feng
author_sort Shan Luo
collection DOAJ
description Background: Emerging infectious diseases threaten human and animal health, with most pathogens originating from wildlife. Bats are natural hosts for many infectious agents. Previous studies have demonstrated that changes in some specific genes in bats may contribute to resistance to viral infections, but they have mostly overlooked the immune function of the bat gut microbiota. Aims: In this study, we used fecal transplants to transfer the gut microbiota from the Greater Horseshoe Bat (<i>Rhinolophus ferrumequinum</i>) into mice treated with antibiotics. The gut microbiota changes in mice were detected using 16S rRNA high-throughput sequencing technology. Flow cytometry was used to detect changes in associated immune cells in the spleen and mesenteric lymph nodes of the mice. Results: The results showed that the gut microbiota of mice showed characteristics of some bat gut microbiota. The Greater Horseshoe Bat’s gut microbiota changed some immune cells’ composition in the spleen and mesenteric lymph nodes of mice and also conferred a faster and higher proportion of natural killer cell activation. Conclusion: This result provides new evidence for the regulatory immune function of bat gut microbiota and contributes to a deeper insight into the unique immune system of bats.
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issn 2076-2615
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series Animals
spelling doaj-art-c70e9227d0a844ef976341cee567db4c2025-08-20T02:52:48ZengMDPI AGAnimals2076-26152025-02-0115568510.3390/ani15050685The Gut Microbiota of the Greater Horseshoe Bat Confers Rapidly Corresponding Immune Cells in MiceShan Luo0Xinlei Huang1Siyu Chen2Junyi Li3Hui Wu4Yuhua He5Lei Zhou6Boyu Liu7Jiang Feng8College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132109, ChinaCollege of Life Science, Jilin Agricultural University, Changchun 130117, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132109, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun 130117, ChinaCollege of Life Science, Jilin Agricultural University, Changchun 130117, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132109, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132109, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132109, ChinaCollege of Life Science, Jilin Agricultural University, Changchun 130117, ChinaBackground: Emerging infectious diseases threaten human and animal health, with most pathogens originating from wildlife. Bats are natural hosts for many infectious agents. Previous studies have demonstrated that changes in some specific genes in bats may contribute to resistance to viral infections, but they have mostly overlooked the immune function of the bat gut microbiota. Aims: In this study, we used fecal transplants to transfer the gut microbiota from the Greater Horseshoe Bat (<i>Rhinolophus ferrumequinum</i>) into mice treated with antibiotics. The gut microbiota changes in mice were detected using 16S rRNA high-throughput sequencing technology. Flow cytometry was used to detect changes in associated immune cells in the spleen and mesenteric lymph nodes of the mice. Results: The results showed that the gut microbiota of mice showed characteristics of some bat gut microbiota. The Greater Horseshoe Bat’s gut microbiota changed some immune cells’ composition in the spleen and mesenteric lymph nodes of mice and also conferred a faster and higher proportion of natural killer cell activation. Conclusion: This result provides new evidence for the regulatory immune function of bat gut microbiota and contributes to a deeper insight into the unique immune system of bats.https://www.mdpi.com/2076-2615/15/5/685bats<i>Chiroptera</i>microbiotaimmunomodulationfecal transplant
spellingShingle Shan Luo
Xinlei Huang
Siyu Chen
Junyi Li
Hui Wu
Yuhua He
Lei Zhou
Boyu Liu
Jiang Feng
The Gut Microbiota of the Greater Horseshoe Bat Confers Rapidly Corresponding Immune Cells in Mice
Animals
bats
<i>Chiroptera</i>
microbiota
immunomodulation
fecal transplant
title The Gut Microbiota of the Greater Horseshoe Bat Confers Rapidly Corresponding Immune Cells in Mice
title_full The Gut Microbiota of the Greater Horseshoe Bat Confers Rapidly Corresponding Immune Cells in Mice
title_fullStr The Gut Microbiota of the Greater Horseshoe Bat Confers Rapidly Corresponding Immune Cells in Mice
title_full_unstemmed The Gut Microbiota of the Greater Horseshoe Bat Confers Rapidly Corresponding Immune Cells in Mice
title_short The Gut Microbiota of the Greater Horseshoe Bat Confers Rapidly Corresponding Immune Cells in Mice
title_sort gut microbiota of the greater horseshoe bat confers rapidly corresponding immune cells in mice
topic bats
<i>Chiroptera</i>
microbiota
immunomodulation
fecal transplant
url https://www.mdpi.com/2076-2615/15/5/685
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