The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress.
Checkpoint activation during S phase modulates transcription. In response to replication arrest, the fission yeast Cds1 checkpoint kinase maintains the normal S-phase transcriptional program by regulating MBF, the S-phase transcription factor. We show that similar regulation occurs in response to DN...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2009-09-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0006944&type=printable |
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| author | Chaitali Dutta Nicholas Rhind |
| author_facet | Chaitali Dutta Nicholas Rhind |
| author_sort | Chaitali Dutta |
| collection | DOAJ |
| description | Checkpoint activation during S phase modulates transcription. In response to replication arrest, the fission yeast Cds1 checkpoint kinase maintains the normal S-phase transcriptional program by regulating MBF, the S-phase transcription factor. We show that similar regulation occurs in response to DNA damage during S-phase. We test the relative contributions to replication-stress resistance of transcriptional regulation and the two other major checkpoint functions: cell-cycle arrest and fork stabilization. We show that, although transcriptional regulation provides only modest resistance relative to fork stabilization, it contributes significantly to cell survival. Finally, we investigate the roles of two specific transcripts: mik1 and mrc1. These results demonstrate the general importance of checkpoint regulation of G1/S transcription in response to replicative stress and elucidate the specific roles of Mik1 and Mrc1 in the checkpoint. |
| format | Article |
| id | doaj-art-c707b6f2b5dd44829bd57199cd38cbfa |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2009-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-c707b6f2b5dd44829bd57199cd38cbfa2025-08-20T02:01:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-09-0149e694410.1371/journal.pone.0006944The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress.Chaitali DuttaNicholas RhindCheckpoint activation during S phase modulates transcription. In response to replication arrest, the fission yeast Cds1 checkpoint kinase maintains the normal S-phase transcriptional program by regulating MBF, the S-phase transcription factor. We show that similar regulation occurs in response to DNA damage during S-phase. We test the relative contributions to replication-stress resistance of transcriptional regulation and the two other major checkpoint functions: cell-cycle arrest and fork stabilization. We show that, although transcriptional regulation provides only modest resistance relative to fork stabilization, it contributes significantly to cell survival. Finally, we investigate the roles of two specific transcripts: mik1 and mrc1. These results demonstrate the general importance of checkpoint regulation of G1/S transcription in response to replicative stress and elucidate the specific roles of Mik1 and Mrc1 in the checkpoint.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0006944&type=printable |
| spellingShingle | Chaitali Dutta Nicholas Rhind The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress. PLoS ONE |
| title | The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress. |
| title_full | The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress. |
| title_fullStr | The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress. |
| title_full_unstemmed | The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress. |
| title_short | The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress. |
| title_sort | role of specific checkpoint induced s phase transcripts in resistance to replicative stress |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0006944&type=printable |
| work_keys_str_mv | AT chaitalidutta theroleofspecificcheckpointinducedsphasetranscriptsinresistancetoreplicativestress AT nicholasrhind theroleofspecificcheckpointinducedsphasetranscriptsinresistancetoreplicativestress AT chaitalidutta roleofspecificcheckpointinducedsphasetranscriptsinresistancetoreplicativestress AT nicholasrhind roleofspecificcheckpointinducedsphasetranscriptsinresistancetoreplicativestress |