An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum
Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this study, an inhibitive enzyme assay for heavy metals has been de...
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/678356 |
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| author | Gunasekaran Baskaran Noor Azlina Masdor Mohd Arif Syed Mohd Yunus Shukor |
| author_facet | Gunasekaran Baskaran Noor Azlina Masdor Mohd Arif Syed Mohd Yunus Shukor |
| author_sort | Gunasekaran Baskaran |
| collection | DOAJ |
| description | Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this study, an inhibitive enzyme assay for heavy metals has been developed using crude proteases from Coriandrum sativum. In this assay, casein was used as a substrate and Coomassie dye was used to denote the completion of casein hydrolysis. In the absence of inhibitors, casein was hydrolysed and the solution became brown, while in the presence of metal ions such as Hg2+ and Zn2+, the hydrolysis of casein was inhibited and the solution remained blue. Both Hg2+ and Zn2+ exhibited one-phase binding curve with IC50 values of 3.217 mg/L and 0.727 mg/L, respectively. The limits of detection (LOD) and limits of quantitation (LOQ) for Hg were 0.241 and 0.802 mg/L, respectively, while the LOD and LOQ for Zn were 0.228 and 0.761 mg/L, respectively. The enzyme exhibited broad pH ranges for activity. The crude proteases extracted from Coriandrum sativum showed good potential for the development of a rapid, sensitive, and economic inhibitive assay for the biomonitoring of Hg2+ and Zn2+ in the aquatic environments. |
| format | Article |
| id | doaj-art-c705ced7e58f40458dfc6697b392787f |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-c705ced7e58f40458dfc6697b392787f2025-02-03T01:31:35ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/678356678356An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativumGunasekaran Baskaran0Noor Azlina Masdor1Mohd Arif Syed2Mohd Yunus Shukor3Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaBiotechnology Research Centre, MARDI, P.O. Box 12301, 50774 Kuala Lumpur, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaHeavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this study, an inhibitive enzyme assay for heavy metals has been developed using crude proteases from Coriandrum sativum. In this assay, casein was used as a substrate and Coomassie dye was used to denote the completion of casein hydrolysis. In the absence of inhibitors, casein was hydrolysed and the solution became brown, while in the presence of metal ions such as Hg2+ and Zn2+, the hydrolysis of casein was inhibited and the solution remained blue. Both Hg2+ and Zn2+ exhibited one-phase binding curve with IC50 values of 3.217 mg/L and 0.727 mg/L, respectively. The limits of detection (LOD) and limits of quantitation (LOQ) for Hg were 0.241 and 0.802 mg/L, respectively, while the LOD and LOQ for Zn were 0.228 and 0.761 mg/L, respectively. The enzyme exhibited broad pH ranges for activity. The crude proteases extracted from Coriandrum sativum showed good potential for the development of a rapid, sensitive, and economic inhibitive assay for the biomonitoring of Hg2+ and Zn2+ in the aquatic environments.http://dx.doi.org/10.1155/2013/678356 |
| spellingShingle | Gunasekaran Baskaran Noor Azlina Masdor Mohd Arif Syed Mohd Yunus Shukor An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum The Scientific World Journal |
| title | An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum |
| title_full | An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum |
| title_fullStr | An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum |
| title_full_unstemmed | An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum |
| title_short | An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum |
| title_sort | inhibitive enzyme assay to detect mercury and zinc using protease from coriandrum sativum |
| url | http://dx.doi.org/10.1155/2013/678356 |
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