Small volume platelet concentrates for neonatal use are more susceptible to shear-induced storage lesion
The impact of the biophysical environment on the platelet storage lesion (PSL) has mainly focused on reduced temperature storage, overlooking the significance of storage-induced shear stress. Shear stress in platelet storage refers to the frictional force acting parallel to the bag surface and exist...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Platelets |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09537104.2024.2389967 |
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| author | Dean Pym Amanda J. Davies Jessica O. Williams Christine Saunders Chloë E. George Philip E. James |
| author_facet | Dean Pym Amanda J. Davies Jessica O. Williams Christine Saunders Chloë E. George Philip E. James |
| author_sort | Dean Pym |
| collection | DOAJ |
| description | The impact of the biophysical environment on the platelet storage lesion (PSL) has mainly focused on reduced temperature storage, overlooking the significance of storage-induced shear stress. Shear stress in platelet storage refers to the frictional force acting parallel to the bag surface and exists solely through the implementation of agitation. This study investigates whether minimizing exposure to agitation-induced shear stress can alleviate the unexplained loss of function in stored platelet concentrates for neonatal transfusion (neonatal PCs). Using particle tracking analysis, fluid motion was measured in neonatal and adult platelet storage bags under agitation frequencies ranging from 20–60 rpm. Platelets stored at 20-60 rpm agitation over 8 days were examined by biochemical analysis, aggregation, and expression of activation markers. Results indicate that neonatal PCs experience significantly higher storage-induced shear stress compared to adult doses, leading to reduced functionality and increased activation from day 2 of storage. Adjusting the neonatal PC agitation frequency to 20 rpm improved functionality in early storage, while 40 rpm maintains this improvement throughout storage with reduced activation, compared to 60 rpm storage. This study confirms that small volume PC storage for neonatal use contributes to the PSL through the induction of shear stress, suggesting further evaluation of the recommended agitation frequency for neonatal PCs or postponement of the production of neonatal PCs until requested for neonatal transfusion. |
| format | Article |
| id | doaj-art-c70292073e1540ffae69e0da718b17ff |
| institution | OA Journals |
| issn | 0953-7104 1369-1635 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj-art-c70292073e1540ffae69e0da718b17ff2025-08-20T02:27:18ZengTaylor & Francis GroupPlatelets0953-71041369-16352024-12-0135110.1080/09537104.2024.2389967Small volume platelet concentrates for neonatal use are more susceptible to shear-induced storage lesionDean Pym0Amanda J. Davies1Jessica O. Williams2Christine Saunders3Chloë E. George4Philip E. James5Centre of Cardiovascular Health and Ageing, Cardiff Metropolitan University, Cardiff, Wales, UKCentre of Cardiovascular Health and Ageing, Cardiff Metropolitan University, Cardiff, Wales, UKCentre of Cardiovascular Health and Ageing, Cardiff Metropolitan University, Cardiff, Wales, UKWelsh Blood Service, Component Development and Research Laboratory, Pontyclun, Wales, UKWelsh Blood Service, Component Development and Research Laboratory, Pontyclun, Wales, UKCentre of Cardiovascular Health and Ageing, Cardiff Metropolitan University, Cardiff, Wales, UKThe impact of the biophysical environment on the platelet storage lesion (PSL) has mainly focused on reduced temperature storage, overlooking the significance of storage-induced shear stress. Shear stress in platelet storage refers to the frictional force acting parallel to the bag surface and exists solely through the implementation of agitation. This study investigates whether minimizing exposure to agitation-induced shear stress can alleviate the unexplained loss of function in stored platelet concentrates for neonatal transfusion (neonatal PCs). Using particle tracking analysis, fluid motion was measured in neonatal and adult platelet storage bags under agitation frequencies ranging from 20–60 rpm. Platelets stored at 20-60 rpm agitation over 8 days were examined by biochemical analysis, aggregation, and expression of activation markers. Results indicate that neonatal PCs experience significantly higher storage-induced shear stress compared to adult doses, leading to reduced functionality and increased activation from day 2 of storage. Adjusting the neonatal PC agitation frequency to 20 rpm improved functionality in early storage, while 40 rpm maintains this improvement throughout storage with reduced activation, compared to 60 rpm storage. This study confirms that small volume PC storage for neonatal use contributes to the PSL through the induction of shear stress, suggesting further evaluation of the recommended agitation frequency for neonatal PCs or postponement of the production of neonatal PCs until requested for neonatal transfusion.https://www.tandfonline.com/doi/10.1080/09537104.2024.2389967Agitationneonatalplateletshear stress |
| spellingShingle | Dean Pym Amanda J. Davies Jessica O. Williams Christine Saunders Chloë E. George Philip E. James Small volume platelet concentrates for neonatal use are more susceptible to shear-induced storage lesion Platelets Agitation neonatal platelet shear stress |
| title | Small volume platelet concentrates for neonatal use are more susceptible to shear-induced storage lesion |
| title_full | Small volume platelet concentrates for neonatal use are more susceptible to shear-induced storage lesion |
| title_fullStr | Small volume platelet concentrates for neonatal use are more susceptible to shear-induced storage lesion |
| title_full_unstemmed | Small volume platelet concentrates for neonatal use are more susceptible to shear-induced storage lesion |
| title_short | Small volume platelet concentrates for neonatal use are more susceptible to shear-induced storage lesion |
| title_sort | small volume platelet concentrates for neonatal use are more susceptible to shear induced storage lesion |
| topic | Agitation neonatal platelet shear stress |
| url | https://www.tandfonline.com/doi/10.1080/09537104.2024.2389967 |
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