Discovery of Novel Thanatin-like Antimicrobial Peptides from Bean Bug <i>Riptortus pedestris</i>
<b>Background:</b> Endogenous antimicrobial peptides (AMPs) are evolutionarily ancient molecular factors of innate immunity that play a key role in host defense. The study of the diversity of animal defense peptides has important applications in the context of the growing global antimicr...
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2024-11-01
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| author | Pavel V. Panteleev Julia S. Teplovodskaya Anastasia D. Utkina Anastasia A. Smolina Roman N. Kruglikov Victoria N. Safronova Ilia A. Bolosov Olga V. Korobova Alexander I. Borzilov Tatiana V. Ovchinnikova |
| author_facet | Pavel V. Panteleev Julia S. Teplovodskaya Anastasia D. Utkina Anastasia A. Smolina Roman N. Kruglikov Victoria N. Safronova Ilia A. Bolosov Olga V. Korobova Alexander I. Borzilov Tatiana V. Ovchinnikova |
| author_sort | Pavel V. Panteleev |
| collection | DOAJ |
| description | <b>Background:</b> Endogenous antimicrobial peptides (AMPs) are evolutionarily ancient molecular factors of innate immunity that play a key role in host defense. The study of the diversity of animal defense peptides has important applications in the context of the growing global antimicrobial resistance. <b>Methods:</b> In this study using a transcriptome mining approach, we found three novel thanatin-like β-hairpin AMPs in the bean bug <i>Riptortus pedestris</i>, named Rip-2, Rip-3, and Rip-4. The peptides were expressed in the bacterial system, and their antimicrobial activities were evaluated both in vitro and in vivo. <b>Results:</b> Homologs of the discovered AMPs are widely distributed among different members of the infraorder Pentatomomorpha. Rip-2 was shown to have the most similar structure and LptA-targeting mechanism of action to those of thanatin, but the former peptides demonstrated a higher activity against key Gram-negative ESKAPE pathogens and also displayed a significant efficacy in a lethal model of septicemia caused by <i>E. coli</i> in mice at daily doses greater than 5 mg/kg. In contrast, Rip-3 and Rip-4 peptides caused bacterial membrane damage, did not induce bacterial resistance, and exhibited a strong selectivity against <i>Bacillus</i> and <i>Mycobacterium</i> spp. <b>Conclusions:</b> This study extends the knowledge of the structure and functions of insect host defense AMPs. Each of the novel β-hairpin peptides has a potential to be a template for the development of selective antibiotic drugs. |
| format | Article |
| id | doaj-art-c6f91744bf774d36bd145df3519b6b2f |
| institution | OA Journals |
| issn | 1999-4923 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj-art-c6f91744bf774d36bd145df3519b6b2f2025-08-20T02:05:01ZengMDPI AGPharmaceutics1999-49232024-11-011611145310.3390/pharmaceutics16111453Discovery of Novel Thanatin-like Antimicrobial Peptides from Bean Bug <i>Riptortus pedestris</i>Pavel V. Panteleev0Julia S. Teplovodskaya1Anastasia D. Utkina2Anastasia A. Smolina3Roman N. Kruglikov4Victoria N. Safronova5Ilia A. Bolosov6Olga V. Korobova7Alexander I. Borzilov8Tatiana V. Ovchinnikova9M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaState Research Center for Applied Microbiology & Biotechnology (SRCAMB), 142279 Obolensk, RussiaState Research Center for Applied Microbiology & Biotechnology (SRCAMB), 142279 Obolensk, RussiaM.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia<b>Background:</b> Endogenous antimicrobial peptides (AMPs) are evolutionarily ancient molecular factors of innate immunity that play a key role in host defense. The study of the diversity of animal defense peptides has important applications in the context of the growing global antimicrobial resistance. <b>Methods:</b> In this study using a transcriptome mining approach, we found three novel thanatin-like β-hairpin AMPs in the bean bug <i>Riptortus pedestris</i>, named Rip-2, Rip-3, and Rip-4. The peptides were expressed in the bacterial system, and their antimicrobial activities were evaluated both in vitro and in vivo. <b>Results:</b> Homologs of the discovered AMPs are widely distributed among different members of the infraorder Pentatomomorpha. Rip-2 was shown to have the most similar structure and LptA-targeting mechanism of action to those of thanatin, but the former peptides demonstrated a higher activity against key Gram-negative ESKAPE pathogens and also displayed a significant efficacy in a lethal model of septicemia caused by <i>E. coli</i> in mice at daily doses greater than 5 mg/kg. In contrast, Rip-3 and Rip-4 peptides caused bacterial membrane damage, did not induce bacterial resistance, and exhibited a strong selectivity against <i>Bacillus</i> and <i>Mycobacterium</i> spp. <b>Conclusions:</b> This study extends the knowledge of the structure and functions of insect host defense AMPs. Each of the novel β-hairpin peptides has a potential to be a template for the development of selective antibiotic drugs.https://www.mdpi.com/1999-4923/16/11/1453antimicrobial peptidethanatin<i>Riptortus pedestris</i>transcriptome miningin vivo efficacyLptA |
| spellingShingle | Pavel V. Panteleev Julia S. Teplovodskaya Anastasia D. Utkina Anastasia A. Smolina Roman N. Kruglikov Victoria N. Safronova Ilia A. Bolosov Olga V. Korobova Alexander I. Borzilov Tatiana V. Ovchinnikova Discovery of Novel Thanatin-like Antimicrobial Peptides from Bean Bug <i>Riptortus pedestris</i> Pharmaceutics antimicrobial peptide thanatin <i>Riptortus pedestris</i> transcriptome mining in vivo efficacy LptA |
| title | Discovery of Novel Thanatin-like Antimicrobial Peptides from Bean Bug <i>Riptortus pedestris</i> |
| title_full | Discovery of Novel Thanatin-like Antimicrobial Peptides from Bean Bug <i>Riptortus pedestris</i> |
| title_fullStr | Discovery of Novel Thanatin-like Antimicrobial Peptides from Bean Bug <i>Riptortus pedestris</i> |
| title_full_unstemmed | Discovery of Novel Thanatin-like Antimicrobial Peptides from Bean Bug <i>Riptortus pedestris</i> |
| title_short | Discovery of Novel Thanatin-like Antimicrobial Peptides from Bean Bug <i>Riptortus pedestris</i> |
| title_sort | discovery of novel thanatin like antimicrobial peptides from bean bug i riptortus pedestris i |
| topic | antimicrobial peptide thanatin <i>Riptortus pedestris</i> transcriptome mining in vivo efficacy LptA |
| url | https://www.mdpi.com/1999-4923/16/11/1453 |
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