Differentiation of soil metabolic function and microbial communities between plantations and natural reforestation
Reforestation plays a vital role in restoring the soil degradation areas. However, the mechanisms by which different restoration approaches affect the soil properties and microbial communities remain unclear. Aiming to understand the interactions between plant species, soil properties, and microbial...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544641/full |
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| author | Nannan Zhang Nannan Zhang Xiaoxia Chen Xiaoxia Chen Xiaoxia Chen Tingju Ren Tingju Ren Jiangcheng Luo Jiangcheng Luo Jin Liang Jin Liang En Tao Wang Fusun Shi Fusun Shi |
| author_facet | Nannan Zhang Nannan Zhang Xiaoxia Chen Xiaoxia Chen Xiaoxia Chen Tingju Ren Tingju Ren Jiangcheng Luo Jiangcheng Luo Jin Liang Jin Liang En Tao Wang Fusun Shi Fusun Shi |
| author_sort | Nannan Zhang |
| collection | DOAJ |
| description | Reforestation plays a vital role in restoring the soil degradation areas. However, the mechanisms by which different restoration approaches affect the soil properties and microbial communities remain unclear. Aiming to understand the interactions between plant species, soil properties, and microbial communities in different restoration approaches, we investigated the soil microbial community using nontargeted metabolomics to explore how the reforestation approach affects soil physicochemical properties, soil metabolites, and soil microbial communities. The results showed that the reforestation approach, soil layer, and their interactive effects significantly affected soil organic carbon, total nitrogen, dissolved organic carbon, available phosphorus concentrations, and root traits. The diversity and composition of bacterial and fungal communities in natural reforestation (NR) were different from those in artificial mono-plantations, and their network interactions were more significant in NR than in artificial plantations. A clear separation of metabolites between the artificial plantations and NR was observed in the soil metabolite analysis. Two pathways, linoleic acid metabolism, and valine, leucine, and isoleucine biosynthesis, were significantly regulated between the artificial mono-plantations and NR. Different soil traits were significantly correlated with dominant microbial taxa in the four reforestation approaches. 13-L-hydroperoxylinoleic acid, 13-S-hydroxyoctadecadienoic acid, homovanillin, and 9,10-epoxyoctadecenoic acid showed the highest correlation with the microbial taxa in the network. Partial least squares path modeling (PLS-PM) shows that root-mediated soil physicochemical properties were the primary factors affecting the bacterial community among the reforestation approaches. The soil fungal community is directly regulated by plant roots in the subsoil and indirectly regulated by the root-mediated physicochemical properties in the topsoil. We conclude that different reforestation approaches affect the soil microbial community through root and soil physicochemical properties rather than soil metabolites. |
| format | Article |
| id | doaj-art-c6f2b871ef4c4578bf4443e95259a3f5 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-c6f2b871ef4c4578bf4443e95259a3f52025-08-20T03:04:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15446411544641Differentiation of soil metabolic function and microbial communities between plantations and natural reforestationNannan Zhang0Nannan Zhang1Xiaoxia Chen2Xiaoxia Chen3Xiaoxia Chen4Tingju Ren5Tingju Ren6Jiangcheng Luo7Jiangcheng Luo8Jin Liang9Jin Liang10En Tao Wang11Fusun Shi12Fusun Shi13Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaCollege of Life Science, Sichuan Normal University, Chengdu, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaEscuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MexicoChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaReforestation plays a vital role in restoring the soil degradation areas. However, the mechanisms by which different restoration approaches affect the soil properties and microbial communities remain unclear. Aiming to understand the interactions between plant species, soil properties, and microbial communities in different restoration approaches, we investigated the soil microbial community using nontargeted metabolomics to explore how the reforestation approach affects soil physicochemical properties, soil metabolites, and soil microbial communities. The results showed that the reforestation approach, soil layer, and their interactive effects significantly affected soil organic carbon, total nitrogen, dissolved organic carbon, available phosphorus concentrations, and root traits. The diversity and composition of bacterial and fungal communities in natural reforestation (NR) were different from those in artificial mono-plantations, and their network interactions were more significant in NR than in artificial plantations. A clear separation of metabolites between the artificial plantations and NR was observed in the soil metabolite analysis. Two pathways, linoleic acid metabolism, and valine, leucine, and isoleucine biosynthesis, were significantly regulated between the artificial mono-plantations and NR. Different soil traits were significantly correlated with dominant microbial taxa in the four reforestation approaches. 13-L-hydroperoxylinoleic acid, 13-S-hydroxyoctadecadienoic acid, homovanillin, and 9,10-epoxyoctadecenoic acid showed the highest correlation with the microbial taxa in the network. Partial least squares path modeling (PLS-PM) shows that root-mediated soil physicochemical properties were the primary factors affecting the bacterial community among the reforestation approaches. The soil fungal community is directly regulated by plant roots in the subsoil and indirectly regulated by the root-mediated physicochemical properties in the topsoil. We conclude that different reforestation approaches affect the soil microbial community through root and soil physicochemical properties rather than soil metabolites.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544641/fullmicrobiomemetabolomicsplant-microbial interactionmono-planationnatural reforestation |
| spellingShingle | Nannan Zhang Nannan Zhang Xiaoxia Chen Xiaoxia Chen Xiaoxia Chen Tingju Ren Tingju Ren Jiangcheng Luo Jiangcheng Luo Jin Liang Jin Liang En Tao Wang Fusun Shi Fusun Shi Differentiation of soil metabolic function and microbial communities between plantations and natural reforestation Frontiers in Microbiology microbiome metabolomics plant-microbial interaction mono-planation natural reforestation |
| title | Differentiation of soil metabolic function and microbial communities between plantations and natural reforestation |
| title_full | Differentiation of soil metabolic function and microbial communities between plantations and natural reforestation |
| title_fullStr | Differentiation of soil metabolic function and microbial communities between plantations and natural reforestation |
| title_full_unstemmed | Differentiation of soil metabolic function and microbial communities between plantations and natural reforestation |
| title_short | Differentiation of soil metabolic function and microbial communities between plantations and natural reforestation |
| title_sort | differentiation of soil metabolic function and microbial communities between plantations and natural reforestation |
| topic | microbiome metabolomics plant-microbial interaction mono-planation natural reforestation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544641/full |
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