Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in Goats
Six rumen-cannulated lactating Guanzhong goats were used to investigate changes in ruminal fermentation pattern and the microbiota following a subacute ruminal acidosis (SARA) inducing procedure. Induction of SARA was performed by increasing dietary non-fiber carbohydrate (NFC) to neutral detergent...
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KeAi Communications Co., Ltd.
2013-02-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913602312 |
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| author | Da-cheng LIU Xiang-li ZHOU Pei-ting ZHAO Min GAO Hao-qi HAN Hong-lian HU |
| author_facet | Da-cheng LIU Xiang-li ZHOU Pei-ting ZHAO Min GAO Hao-qi HAN Hong-lian HU |
| author_sort | Da-cheng LIU |
| collection | DOAJ |
| description | Six rumen-cannulated lactating Guanzhong goats were used to investigate changes in ruminal fermentation pattern and the microbiota following a subacute ruminal acidosis (SARA) inducing procedure. Induction of SARA was performed by increasing dietary non-fiber carbohydrate (NFC) to neutral detergent fiber (NDF) ratio from 1.02 (stage 1) to 1.24 (stage 2), 1.63 (stage 3) and 2.58 (stage 4). A dynamic pH monitoring system, real-time fluorescent quantitative PCR and conventional anaerobic culture were used to assess changes in ruminal pH and microbiota. Results indicated that rumen fermentation patterns changed significantly with increased NFC: NDF ratio. The decline in ruminal pH was caused by increased ruminal total volatile fatty acids (TVFA), which was mainly attributed to a significant increase in ruminal butyrate, rather than the accumulation of ruminal lactic acid. In addition, in the course of SARA, the number of rumen microoganisms altered significantly, with increases in ruminal amylolytic bacteria, Lactobacilli, Streptococcus bovis and Megasphaera elsdenii, the latter particularly dramatically indicating that it may be the main factor responsible for the increase in butyrate, and decrease in protozoa. |
| format | Article |
| id | doaj-art-c6f05530ce4d42fdbd5643d1fb008b9a |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2013-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-c6f05530ce4d42fdbd5643d1fb008b9a2025-08-20T03:57:47ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192013-02-0112231932610.1016/S2095-3119(13)60231-2Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in GoatsDa-cheng LIU0Xiang-li ZHOU1Pei-ting ZHAO2Min GAO3Hao-qi HAN4Hong-lian HU5College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 010018, P.R. China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease of Ministry of Agriculture, Huhhot 010018, P.R. China; Correspondence LIU Da-cheng, Mobile: 13664816212, Fax: +86-471-4309176College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 010018, P.R. China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease of Ministry of Agriculture, Huhhot 010018, P.R. ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 010018, P.R. China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease of Ministry of Agriculture, Huhhot 010018, P.R. ChinaAnimal Nutrition Institute of Agriculture and Animal Husbandry, Academy of Inner Mongolia, Huhhot 010031, P.R. ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 010018, P.R. China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease of Ministry of Agriculture, Huhhot 010018, P.R. ChinaAnimal Nutrition Institute of Agriculture and Animal Husbandry, Academy of Inner Mongolia, Huhhot 010031, P.R. ChinaSix rumen-cannulated lactating Guanzhong goats were used to investigate changes in ruminal fermentation pattern and the microbiota following a subacute ruminal acidosis (SARA) inducing procedure. Induction of SARA was performed by increasing dietary non-fiber carbohydrate (NFC) to neutral detergent fiber (NDF) ratio from 1.02 (stage 1) to 1.24 (stage 2), 1.63 (stage 3) and 2.58 (stage 4). A dynamic pH monitoring system, real-time fluorescent quantitative PCR and conventional anaerobic culture were used to assess changes in ruminal pH and microbiota. Results indicated that rumen fermentation patterns changed significantly with increased NFC: NDF ratio. The decline in ruminal pH was caused by increased ruminal total volatile fatty acids (TVFA), which was mainly attributed to a significant increase in ruminal butyrate, rather than the accumulation of ruminal lactic acid. In addition, in the course of SARA, the number of rumen microoganisms altered significantly, with increases in ruminal amylolytic bacteria, Lactobacilli, Streptococcus bovis and Megasphaera elsdenii, the latter particularly dramatically indicating that it may be the main factor responsible for the increase in butyrate, and decrease in protozoa.http://www.sciencedirect.com/science/article/pii/S2095311913602312rumen fermentationrumen microorganismssubacute ruminal acidosisgoat |
| spellingShingle | Da-cheng LIU Xiang-li ZHOU Pei-ting ZHAO Min GAO Hao-qi HAN Hong-lian HU Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in Goats Journal of Integrative Agriculture rumen fermentation rumen microorganisms subacute ruminal acidosis goat |
| title | Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in Goats |
| title_full | Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in Goats |
| title_fullStr | Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in Goats |
| title_full_unstemmed | Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in Goats |
| title_short | Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in Goats |
| title_sort | effects of increasing non fiber carbohydrate to neutral detergent fiber ratio on rumen fermentation and microbiota in goats |
| topic | rumen fermentation rumen microorganisms subacute ruminal acidosis goat |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913602312 |
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