Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran
Introduction: This study aimed to determine the frequency of extendedspectrum beta-lactamases (ESBL) and different drug resistance phenotypes in Enterobacteriaceae isolated from clinical specimens in Gonbad-e Kavus, Golestan Province. Methods: 220 clinical samples of urine, blood, pus, sputum, C...
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Pasteur Institute of Iran
2022-03-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
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| Online Access: | https://jommid.pasteur.ac.ir/article-1-442-en.html |
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| author | Mohammad Hossein Akbari1 Ania Ahani Azari2 Leila Fozouni2 |
| author_facet | Mohammad Hossein Akbari1 Ania Ahani Azari2 Leila Fozouni2 |
| author_sort | Mohammad Hossein Akbari1 |
| collection | DOAJ |
| description | Introduction: This study aimed to determine the frequency of extendedspectrum
beta-lactamases (ESBL) and different drug resistance phenotypes in
Enterobacteriaceae isolated from clinical specimens in Gonbad-e Kavus,
Golestan Province. Methods: 220 clinical samples of urine, blood, pus, sputum,
CSF, body fluids, and ear and eye discharge were collected during six months
from April to September 2021 at a referral hospital. The samples were cultured
on blood and MacConkey agar and incubated overnight at 37 °C. Standard
biochemical tests and the API20E enteric identification system were used to
identify bacteria. The Kirby-Bauer disk diffusion method determined the
antibiotic resistance pattern, and the phenotypic confirmatory test was used for
detecting ESBL producers. Results: 108 Enterobacteriaceae isolates were
identified from different clinical specimens out of the samples. The isolates were
Escherichia coli (36.1%), Klebsiella pneumonia (25%), Enterobacter cloacae
(18.5%), Citrobacter freundii (11.1%) and Proteus mirabilis (9.2%). The
highest resistance and susceptibility among the isolates belonged to
sulfamethoxazole-trimethoprim (68.5%) and meropenem (11.1%), respectively.
The highest prevalence of multidrug-resistant (MDR) and ESBL were observed
in E. coli and Proteus mirabilis isolates. Conclusion: In this study, the high
frequency of MDR phenotypes in the isolates may suggest an increasing trend
of antibiotic resistance in Enterobacteriaceae. This could greatly impact the
management and treatment of infections caused by these drug-resistant bacteria.
Therefore, infection-control measures and continuous monitoring is
recommended for controlling the spread of ESBL-producing strains in different
geographical areas. |
| format | Article |
| id | doaj-art-c6e1b44af837464489736ecdec50d18f |
| institution | OA Journals |
| issn | 2345-5349 2345-5330 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj-art-c6e1b44af837464489736ecdec50d18f2025-08-20T02:16:04ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302022-03-01101192310.52547/JoMMID.10.1.19Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, IranMohammad Hossein Akbari10https://orcid.org/0000-0003-2807-0580Ania Ahani Azari21https://orcid.org/0000-0001-7025-6739Leila Fozouni22https://orcid.org/0000-0002-2248-03541MSc, Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran2PhD, Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran.2PhD, Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran.Introduction: This study aimed to determine the frequency of extendedspectrum beta-lactamases (ESBL) and different drug resistance phenotypes in Enterobacteriaceae isolated from clinical specimens in Gonbad-e Kavus, Golestan Province. Methods: 220 clinical samples of urine, blood, pus, sputum, CSF, body fluids, and ear and eye discharge were collected during six months from April to September 2021 at a referral hospital. The samples were cultured on blood and MacConkey agar and incubated overnight at 37 °C. Standard biochemical tests and the API20E enteric identification system were used to identify bacteria. The Kirby-Bauer disk diffusion method determined the antibiotic resistance pattern, and the phenotypic confirmatory test was used for detecting ESBL producers. Results: 108 Enterobacteriaceae isolates were identified from different clinical specimens out of the samples. The isolates were Escherichia coli (36.1%), Klebsiella pneumonia (25%), Enterobacter cloacae (18.5%), Citrobacter freundii (11.1%) and Proteus mirabilis (9.2%). The highest resistance and susceptibility among the isolates belonged to sulfamethoxazole-trimethoprim (68.5%) and meropenem (11.1%), respectively. The highest prevalence of multidrug-resistant (MDR) and ESBL were observed in E. coli and Proteus mirabilis isolates. Conclusion: In this study, the high frequency of MDR phenotypes in the isolates may suggest an increasing trend of antibiotic resistance in Enterobacteriaceae. This could greatly impact the management and treatment of infections caused by these drug-resistant bacteria. Therefore, infection-control measures and continuous monitoring is recommended for controlling the spread of ESBL-producing strains in different geographical areas.https://jommid.pasteur.ac.ir/article-1-442-en.htmlbeta-lactamasesdrugresistantenterobacteriaceaeprevalence |
| spellingShingle | Mohammad Hossein Akbari1 Ania Ahani Azari2 Leila Fozouni2 Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran Journal of Medical Microbiology and Infectious Diseases beta-lactamases drugresistant enterobacteriaceae prevalence |
| title | Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran |
| title_full | Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran |
| title_fullStr | Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran |
| title_full_unstemmed | Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran |
| title_short | Broad-Spectrum Beta-Lactamases and Drug-Resistance Phenotypes of Enterobacteriaceae Isolated from Clinical Specimens in Gonbad-e Kavus, Golestan Province, Iran |
| title_sort | broad spectrum beta lactamases and drug resistance phenotypes of enterobacteriaceae isolated from clinical specimens in gonbad e kavus golestan province iran |
| topic | beta-lactamases drugresistant enterobacteriaceae prevalence |
| url | https://jommid.pasteur.ac.ir/article-1-442-en.html |
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