The high-quality telomere-to-telomere genome assembly of the earthworm (Amynthas aspergillum)

Abstract Earthworms have been extensively studied as ancient soil invertebrates, that are highly diverse. Previous studies of these invertebrates have mainly focused on their ecosystem services, medicinal value, and ecological habits. However, their genomic analysis remains inadequate. In this study...

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Main Authors: Guangquan Peng, Yanghe Qin, Zhiming Yan, Mingwei He, Yucheng Zhao, Neng Jiang, Changhong Wei
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05058-w
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author Guangquan Peng
Yanghe Qin
Zhiming Yan
Mingwei He
Yucheng Zhao
Neng Jiang
Changhong Wei
author_facet Guangquan Peng
Yanghe Qin
Zhiming Yan
Mingwei He
Yucheng Zhao
Neng Jiang
Changhong Wei
author_sort Guangquan Peng
collection DOAJ
description Abstract Earthworms have been extensively studied as ancient soil invertebrates, that are highly diverse. Previous studies of these invertebrates have mainly focused on their ecosystem services, medicinal value, and ecological habits. However, their genomic analysis remains inadequate. In this study, we generated the first high-quality telomere-to-telomere (T2T) assembly of the genome of the earthworm, Amynthas aspergillum (Perrier, 1872), which belongs to the genus Amynthas of the family Megascolecidae. The T2T assembly was 758.86 Mb with an N50 contig size of 16.59 Mb. The sequences were anchored to 43 chromosomes (2n = 2x = 86) with a coverage of 98.43% (746.95 Mb), and 83 telomeres were detected. In addition, we also predicted 35,723 protein-coding genes with 97.02% being functionally annotated. This T2T genome assembly will establish substantial groundwork for exploring the evolutionary mechanisms of the earthworm genome and enhance the specificity of its pharmacological effects.
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issn 2052-4463
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publishDate 2025-06-01
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spelling doaj-art-c6d7afd1adcd40cfb88fae0fb397289f2025-08-20T02:30:43ZengNature PortfolioScientific Data2052-44632025-06-011211910.1038/s41597-025-05058-wThe high-quality telomere-to-telomere genome assembly of the earthworm (Amynthas aspergillum)Guangquan Peng0Yanghe Qin1Zhiming Yan2Mingwei He3Yucheng Zhao4Neng Jiang5Changhong Wei6Department of Research & Clinical Laboratory, The Fifth Affiliated Hospital of Guangxi Medical University & The First People’s Hospital of NanningDepartment of Pharmacy, Guangxi Medical University Cancer HospitalDepartment of Pharmacy, Guangxi Medical University Cancer HospitalDepartment of Pharmacy, Guangxi Medical University Cancer HospitalState Key Laboratory of Natural Medicines, Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical UniversityDepartment of Pharmacy, Guangxi Medical University Cancer HospitalDepartment of Research & Clinical Laboratory, The Fifth Affiliated Hospital of Guangxi Medical University & The First People’s Hospital of NanningAbstract Earthworms have been extensively studied as ancient soil invertebrates, that are highly diverse. Previous studies of these invertebrates have mainly focused on their ecosystem services, medicinal value, and ecological habits. However, their genomic analysis remains inadequate. In this study, we generated the first high-quality telomere-to-telomere (T2T) assembly of the genome of the earthworm, Amynthas aspergillum (Perrier, 1872), which belongs to the genus Amynthas of the family Megascolecidae. The T2T assembly was 758.86 Mb with an N50 contig size of 16.59 Mb. The sequences were anchored to 43 chromosomes (2n = 2x = 86) with a coverage of 98.43% (746.95 Mb), and 83 telomeres were detected. In addition, we also predicted 35,723 protein-coding genes with 97.02% being functionally annotated. This T2T genome assembly will establish substantial groundwork for exploring the evolutionary mechanisms of the earthworm genome and enhance the specificity of its pharmacological effects.https://doi.org/10.1038/s41597-025-05058-w
spellingShingle Guangquan Peng
Yanghe Qin
Zhiming Yan
Mingwei He
Yucheng Zhao
Neng Jiang
Changhong Wei
The high-quality telomere-to-telomere genome assembly of the earthworm (Amynthas aspergillum)
Scientific Data
title The high-quality telomere-to-telomere genome assembly of the earthworm (Amynthas aspergillum)
title_full The high-quality telomere-to-telomere genome assembly of the earthworm (Amynthas aspergillum)
title_fullStr The high-quality telomere-to-telomere genome assembly of the earthworm (Amynthas aspergillum)
title_full_unstemmed The high-quality telomere-to-telomere genome assembly of the earthworm (Amynthas aspergillum)
title_short The high-quality telomere-to-telomere genome assembly of the earthworm (Amynthas aspergillum)
title_sort high quality telomere to telomere genome assembly of the earthworm amynthas aspergillum
url https://doi.org/10.1038/s41597-025-05058-w
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