Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse model
Abstract Chronic inflammatory pain is often caused by peripheral tissue damage and persistent inflammation. This disease substantially affects patients’ physical and social well-being. We investigated the role of metallothionein-3 (MT3) in modulating complete Freund’s adjuvant (CFA)-induced intracel...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-02-01
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| Series: | Cell Death Discovery |
| Online Access: | https://doi.org/10.1038/s41420-025-02322-1 |
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| author | Ngoc Buu Tran Sook-Jeong Lee |
| author_facet | Ngoc Buu Tran Sook-Jeong Lee |
| author_sort | Ngoc Buu Tran |
| collection | DOAJ |
| description | Abstract Chronic inflammatory pain is often caused by peripheral tissue damage and persistent inflammation. This disease substantially affects patients’ physical and social well-being. We investigated the role of metallothionein-3 (MT3) in modulating complete Freund’s adjuvant (CFA)-induced intracellular Zn2+ activity in an MT3 knockout mouse model of inflammatory pain in the hind paw. The results demonstrated that increasing intracellular Zn2+ levels ameliorate deficits in motor behavior, as well as inflammation in the paw, spleen, and thymus. Furthermore, intracellular Zn2+ was crucial in regulating oxidative stress markers (glutathione, superoxide dismutase, catalase, and malondialdehyde) and inflammatory cytokines, such as tumor necrosis factor-α and interleukin-6, in MT3 knockout mice induced with CFA. This study highlights the critical role of MT3 in coordinating the intracellular interaction with Zn2+, which is vital for the immune systems’s protective functions. These interactions are fundamental for maintaining metal ion homeostasis and regulating the synthesis of various biomolecules in the body. |
| format | Article |
| id | doaj-art-c6d7179db785445e861ad9ec6a98991c |
| institution | Kabale University |
| issn | 2058-7716 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death Discovery |
| spelling | doaj-art-c6d7179db785445e861ad9ec6a98991c2025-02-09T12:12:30ZengNature Publishing GroupCell Death Discovery2058-77162025-02-0111111110.1038/s41420-025-02322-1Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse modelNgoc Buu Tran0Sook-Jeong Lee1Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Jeonbuk National UniversityDepartment of Bioactive Material Sciences and Research Center of Bioactive Materials, Jeonbuk National UniversityAbstract Chronic inflammatory pain is often caused by peripheral tissue damage and persistent inflammation. This disease substantially affects patients’ physical and social well-being. We investigated the role of metallothionein-3 (MT3) in modulating complete Freund’s adjuvant (CFA)-induced intracellular Zn2+ activity in an MT3 knockout mouse model of inflammatory pain in the hind paw. The results demonstrated that increasing intracellular Zn2+ levels ameliorate deficits in motor behavior, as well as inflammation in the paw, spleen, and thymus. Furthermore, intracellular Zn2+ was crucial in regulating oxidative stress markers (glutathione, superoxide dismutase, catalase, and malondialdehyde) and inflammatory cytokines, such as tumor necrosis factor-α and interleukin-6, in MT3 knockout mice induced with CFA. This study highlights the critical role of MT3 in coordinating the intracellular interaction with Zn2+, which is vital for the immune systems’s protective functions. These interactions are fundamental for maintaining metal ion homeostasis and regulating the synthesis of various biomolecules in the body.https://doi.org/10.1038/s41420-025-02322-1 |
| spellingShingle | Ngoc Buu Tran Sook-Jeong Lee Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse model Cell Death Discovery |
| title | Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse model |
| title_full | Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse model |
| title_fullStr | Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse model |
| title_full_unstemmed | Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse model |
| title_short | Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse model |
| title_sort | metallothionein 3 mediated intracellular zinc mediates antioxidant and anti inflammatory responses in the complete freund s adjuvant induced inflammatory pain mouse model |
| url | https://doi.org/10.1038/s41420-025-02322-1 |
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