Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC
BackgroundN6-methyladenosine (m6A) RNA modification is crucial for tumor development and progression; however, which m6A regulators play a pivotal role in head and neck squamous cell carcinoma (HNSCC) remains ambiguous.MethodsUtilizing the Cancer Genome Atlas (TCGA) database, the expression levels o...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2025.1516867/full |
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| author | Yulin Zhang Yixu Wang Jilin Peng Kun Zhao Ling Li Yuan Zhang Ziyu Zhai Sijie Yuan Shichao Li Shichao Li Shichao Li Fanglei Ye Le Wang |
| author_facet | Yulin Zhang Yixu Wang Jilin Peng Kun Zhao Ling Li Yuan Zhang Ziyu Zhai Sijie Yuan Shichao Li Shichao Li Shichao Li Fanglei Ye Le Wang |
| author_sort | Yulin Zhang |
| collection | DOAJ |
| description | BackgroundN6-methyladenosine (m6A) RNA modification is crucial for tumor development and progression; however, which m6A regulators play a pivotal role in head and neck squamous cell carcinoma (HNSCC) remains ambiguous.MethodsUtilizing the Cancer Genome Atlas (TCGA) database, the expression levels of m6A regulators in HNSCC were examined, which led to the identification of heterogeneous nuclear ribonucleoprotein C (HNRNPC) as a key gene. Further experiments were performed in patient samples, stable cell lines, and a murine xenograft tumor model.ResultsA reliable survival risk model of m6A was constructed based on the TCGA database. Gene Expression Omnibus (GEO), normal and tumor tissue microarrays (TMA), and tumor tissue samples from patients with HNSCC were observed that a high level of HNRNPC expression was closely linked to a poor prognosis among patients. Knockdown of HNRNPC in the HNSCC cell lines HSC-3 and CAL-27 resulted in a significant decrease in proliferation, invasion, and malignant transformation abilities. RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation and sequencing (MeRIP-seq) data revealed that HNRNPC is involved in cell differentiation, cell migration and apoptosis. The mouse xenograft model elucidated that HNRNPC can promote tumorigenesis and progression of HNSCC.ConclusionsHNRNPC can serve as a valuable predictor of tumor progression and prognosis in patients with HNSCC. |
| format | Article |
| id | doaj-art-c6aa0e92a7f64af4b511389e3de327cb |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj-art-c6aa0e92a7f64af4b511389e3de327cb2025-02-07T06:49:30ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2025-02-011510.3389/fonc.2025.15168671516867Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCCYulin Zhang0Yixu Wang1Jilin Peng2Kun Zhao3Ling Li4Yuan Zhang5Ziyu Zhai6Sijie Yuan7Shichao Li8Shichao Li9Shichao Li10Fanglei Ye11Le Wang12Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Otolaryngology, Head and Neck Surgery, People’s Hospital, Peking University, Beijing, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Otolaryngology Head and Neck Surgery, Henan Provincial People’s Hospital, Zhengzhou, Henan, ChinaDepartment of Otolaryngology Head and Neck Surgery, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Otolaryngology Head and Neck Surgery, People’s Hospital of Henan University, Zhengzhou, Henan, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaBackgroundN6-methyladenosine (m6A) RNA modification is crucial for tumor development and progression; however, which m6A regulators play a pivotal role in head and neck squamous cell carcinoma (HNSCC) remains ambiguous.MethodsUtilizing the Cancer Genome Atlas (TCGA) database, the expression levels of m6A regulators in HNSCC were examined, which led to the identification of heterogeneous nuclear ribonucleoprotein C (HNRNPC) as a key gene. Further experiments were performed in patient samples, stable cell lines, and a murine xenograft tumor model.ResultsA reliable survival risk model of m6A was constructed based on the TCGA database. Gene Expression Omnibus (GEO), normal and tumor tissue microarrays (TMA), and tumor tissue samples from patients with HNSCC were observed that a high level of HNRNPC expression was closely linked to a poor prognosis among patients. Knockdown of HNRNPC in the HNSCC cell lines HSC-3 and CAL-27 resulted in a significant decrease in proliferation, invasion, and malignant transformation abilities. RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation and sequencing (MeRIP-seq) data revealed that HNRNPC is involved in cell differentiation, cell migration and apoptosis. The mouse xenograft model elucidated that HNRNPC can promote tumorigenesis and progression of HNSCC.ConclusionsHNRNPC can serve as a valuable predictor of tumor progression and prognosis in patients with HNSCC.https://www.frontiersin.org/articles/10.3389/fonc.2025.1516867/fullheterogeneous nuclear ribonucleoprotein Chead and neck squamous cell carcinomaprognostic significancetumor progressionN6-methyladenosine |
| spellingShingle | Yulin Zhang Yixu Wang Jilin Peng Kun Zhao Ling Li Yuan Zhang Ziyu Zhai Sijie Yuan Shichao Li Shichao Li Shichao Li Fanglei Ye Le Wang Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC Frontiers in Oncology heterogeneous nuclear ribonucleoprotein C head and neck squamous cell carcinoma prognostic significance tumor progression N6-methyladenosine |
| title | Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC |
| title_full | Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC |
| title_fullStr | Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC |
| title_full_unstemmed | Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC |
| title_short | Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC |
| title_sort | expression and prognostic significance of the m6a rna methylation regulator hnrnpc in hnscc |
| topic | heterogeneous nuclear ribonucleoprotein C head and neck squamous cell carcinoma prognostic significance tumor progression N6-methyladenosine |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2025.1516867/full |
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