Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.

Animal models of Zika virus (ZIKV) are needed to better understand tropism and pathogenesis and to test candidate vaccines and therapies to curtail the pandemic. Humans and rhesus macaques possess similar fetal development and placental biology that is not shared between humans and rodents. We inocu...

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Main Authors: Lark L Coffey, Patricia A Pesavento, Rebekah I Keesler, Anil Singapuri, Jennifer Watanabe, Rie Watanabe, JoAnn Yee, Eliza Bliss-Moreau, Christina Cruzen, Kari L Christe, J Rachel Reader, Wilhelm von Morgenland, Anne M Gibbons, A Mark Allen, Jeff Linnen, Kui Gao, Eric Delwart, Graham Simmons, Mars Stone, Marion Lanteri, Sonia Bakkour, Michael Busch, John Morrison, Koen K A Van Rompay
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171148&type=printable
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author Lark L Coffey
Patricia A Pesavento
Rebekah I Keesler
Anil Singapuri
Jennifer Watanabe
Rie Watanabe
JoAnn Yee
Eliza Bliss-Moreau
Christina Cruzen
Kari L Christe
J Rachel Reader
Wilhelm von Morgenland
Anne M Gibbons
A Mark Allen
Jeff Linnen
Kui Gao
Eric Delwart
Graham Simmons
Mars Stone
Marion Lanteri
Sonia Bakkour
Michael Busch
John Morrison
Koen K A Van Rompay
author_facet Lark L Coffey
Patricia A Pesavento
Rebekah I Keesler
Anil Singapuri
Jennifer Watanabe
Rie Watanabe
JoAnn Yee
Eliza Bliss-Moreau
Christina Cruzen
Kari L Christe
J Rachel Reader
Wilhelm von Morgenland
Anne M Gibbons
A Mark Allen
Jeff Linnen
Kui Gao
Eric Delwart
Graham Simmons
Mars Stone
Marion Lanteri
Sonia Bakkour
Michael Busch
John Morrison
Koen K A Van Rompay
author_sort Lark L Coffey
collection DOAJ
description Animal models of Zika virus (ZIKV) are needed to better understand tropism and pathogenesis and to test candidate vaccines and therapies to curtail the pandemic. Humans and rhesus macaques possess similar fetal development and placental biology that is not shared between humans and rodents. We inoculated 2 non-pregnant rhesus macaques with a 2015 Brazilian ZIKV strain. Consistent with most human infections, the animals experienced no clinical disease but developed short-lived plasma viremias that cleared as neutralizing antibody developed. In 1 animal, viral RNA (vRNA) could be detected longer in whole blood than in plasma. Despite no major histopathologic changes, many adult tissues contained vRNA 14 days post-infection with highest levels in hemolymphatic tissues. These observations warrant further studies to investigate ZIKV persistence and its potential clinical implications for transmission via blood products or tissue and organ transplants.
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spelling doaj-art-c69f8eb9be6748fb943c62f406efbf0f2025-08-20T03:04:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e017114810.1371/journal.pone.0171148Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.Lark L CoffeyPatricia A PesaventoRebekah I KeeslerAnil SingapuriJennifer WatanabeRie WatanabeJoAnn YeeEliza Bliss-MoreauChristina CruzenKari L ChristeJ Rachel ReaderWilhelm von MorgenlandAnne M GibbonsA Mark AllenJeff LinnenKui GaoEric DelwartGraham SimmonsMars StoneMarion LanteriSonia BakkourMichael BuschJohn MorrisonKoen K A Van RompayAnimal models of Zika virus (ZIKV) are needed to better understand tropism and pathogenesis and to test candidate vaccines and therapies to curtail the pandemic. Humans and rhesus macaques possess similar fetal development and placental biology that is not shared between humans and rodents. We inoculated 2 non-pregnant rhesus macaques with a 2015 Brazilian ZIKV strain. Consistent with most human infections, the animals experienced no clinical disease but developed short-lived plasma viremias that cleared as neutralizing antibody developed. In 1 animal, viral RNA (vRNA) could be detected longer in whole blood than in plasma. Despite no major histopathologic changes, many adult tissues contained vRNA 14 days post-infection with highest levels in hemolymphatic tissues. These observations warrant further studies to investigate ZIKV persistence and its potential clinical implications for transmission via blood products or tissue and organ transplants.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171148&type=printable
spellingShingle Lark L Coffey
Patricia A Pesavento
Rebekah I Keesler
Anil Singapuri
Jennifer Watanabe
Rie Watanabe
JoAnn Yee
Eliza Bliss-Moreau
Christina Cruzen
Kari L Christe
J Rachel Reader
Wilhelm von Morgenland
Anne M Gibbons
A Mark Allen
Jeff Linnen
Kui Gao
Eric Delwart
Graham Simmons
Mars Stone
Marion Lanteri
Sonia Bakkour
Michael Busch
John Morrison
Koen K A Van Rompay
Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.
PLoS ONE
title Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.
title_full Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.
title_fullStr Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.
title_full_unstemmed Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.
title_short Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.
title_sort zika virus tissue and blood compartmentalization in acute infection of rhesus macaques
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171148&type=printable
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