Cellular Reprogramming Using Defined Factors and MicroRNAs
Development of human bodies, organs, and tissues contains numerous steps of cellular differentiation including an initial zygote, embryonic stem (ES) cells, three germ layers, and multiple expertized lineages of cells. Induced pluripotent stem (iPS) cells have been recently developed using defined r...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7530942 |
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| author | Takanori Eguchi Takuo Kuboki |
| author_facet | Takanori Eguchi Takuo Kuboki |
| author_sort | Takanori Eguchi |
| collection | DOAJ |
| description | Development of human bodies, organs, and tissues contains numerous steps of cellular differentiation including an initial zygote, embryonic stem (ES) cells, three germ layers, and multiple expertized lineages of cells. Induced pluripotent stem (iPS) cells have been recently developed using defined reprogramming factors such as Nanog, Klf5, Oct3/4 (Pou5f1), Sox2, and Myc. This outstanding innovation is largely changing life science and medicine. Methods of direct reprogramming of cells into myocytes, neurons, chondrocytes, and osteoblasts have been further developed using modified combination of factors such as N-myc, L-myc, Sox9, and microRNAs in defined cell/tissue culture conditions. Mesenchymal stem cells (MSCs) and dental pulp stem cells (DPSCs) are also emerging multipotent stem cells with particular microRNA expression signatures. It was shown that miRNA-720 had a role in cellular reprogramming through targeting the pluripotency factor Nanog and induction of DNA methyltransferases (DNMTs). This review reports histories, topics, and idea of cellular reprogramming. |
| format | Article |
| id | doaj-art-c699644aaeeb4c5798526c95383035bc |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c699644aaeeb4c5798526c95383035bc2025-08-20T03:23:19ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/75309427530942Cellular Reprogramming Using Defined Factors and MicroRNAsTakanori Eguchi0Takuo Kuboki1Division of Molecular and Cell Biology, Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 3 Blackfan Circle, Center for Life Science 6, Boston, MA 02115, USADepartment of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8525, JapanDevelopment of human bodies, organs, and tissues contains numerous steps of cellular differentiation including an initial zygote, embryonic stem (ES) cells, three germ layers, and multiple expertized lineages of cells. Induced pluripotent stem (iPS) cells have been recently developed using defined reprogramming factors such as Nanog, Klf5, Oct3/4 (Pou5f1), Sox2, and Myc. This outstanding innovation is largely changing life science and medicine. Methods of direct reprogramming of cells into myocytes, neurons, chondrocytes, and osteoblasts have been further developed using modified combination of factors such as N-myc, L-myc, Sox9, and microRNAs in defined cell/tissue culture conditions. Mesenchymal stem cells (MSCs) and dental pulp stem cells (DPSCs) are also emerging multipotent stem cells with particular microRNA expression signatures. It was shown that miRNA-720 had a role in cellular reprogramming through targeting the pluripotency factor Nanog and induction of DNA methyltransferases (DNMTs). This review reports histories, topics, and idea of cellular reprogramming.http://dx.doi.org/10.1155/2016/7530942 |
| spellingShingle | Takanori Eguchi Takuo Kuboki Cellular Reprogramming Using Defined Factors and MicroRNAs Stem Cells International |
| title | Cellular Reprogramming Using Defined Factors and MicroRNAs |
| title_full | Cellular Reprogramming Using Defined Factors and MicroRNAs |
| title_fullStr | Cellular Reprogramming Using Defined Factors and MicroRNAs |
| title_full_unstemmed | Cellular Reprogramming Using Defined Factors and MicroRNAs |
| title_short | Cellular Reprogramming Using Defined Factors and MicroRNAs |
| title_sort | cellular reprogramming using defined factors and micrornas |
| url | http://dx.doi.org/10.1155/2016/7530942 |
| work_keys_str_mv | AT takanorieguchi cellularreprogrammingusingdefinedfactorsandmicrornas AT takuokuboki cellularreprogrammingusingdefinedfactorsandmicrornas |