Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goats
Objective The aim of this study was to obtain goat CRTC2 gene sequence and elucidate its biological properties, and further study the impact of overexpression and interference of CRTC2 on the cell differentiation of goat subcutaneous precursor adipocytes. Methods The sequence of goat CRTC2 was clone...
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Asian-Australasian Association of Animal Production Societies
2025-05-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0248.pdf |
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| author | Xuening Li Tingting Hu Ruiwen Li Yanyan Li Yaqiu Lin Yong Wang Wei Liu Youli Wang |
| author_facet | Xuening Li Tingting Hu Ruiwen Li Yanyan Li Yaqiu Lin Yong Wang Wei Liu Youli Wang |
| author_sort | Xuening Li |
| collection | DOAJ |
| description | Objective The aim of this study was to obtain goat CRTC2 gene sequence and elucidate its biological properties, and further study the impact of overexpression and interference of CRTC2 on the cell differentiation of goat subcutaneous precursor adipocytes. Methods The sequence of goat CRTC2 was cloned by reverse transcription (RT)-polymerase chain reaction (PCR) and its molecular characterization was analyzed. The expression of CRTC2 gene in goat tissues and subcutaneous precursor adipocytes differentiated from 0 to 120 h was examined by quantitative real-time PCR (qRT-PCR). The effects of CRTC2 on the subcutaneous precursor adipocyte differentiation were investigated by using liposome transfection, Bodipy, Oil Red O staining and qPCR. Results The results showed that the cloned goat CRTC2 gene was 2363 bp long (coding sequence [CDS] 2082 bp), encoding 693 amino acids. The relative expression levels of CRTC2 gene were highest in liver and then in kidney (p<0.05). During differentiation, the highest expression of CRTC2 in subcutaneous precursor adipocytes was observed at 120 of differentiating (p<0.01). In addition, we found that overexpression of CRTC2 significantly increased the expression of lipid metabolism-related genes (C/EBPα, C/EBPβ, PPARγ, DGAT1, DGAT2, ACC, FASN, SREBP1, AP2, LPL, ATGL) and promoted lipid accumulation. We then chemically synthesized goat CRTC2 small interfering RNA and transfected it into goat subcutaneous precursor adipocytes. The results revealed that SiRNA-mediated interference with CRTC2 significantly inhibited its differentiation and suppressed lipid droplet aggregation. Conclusion So, this study indicates that CRTC2 is a positive regulator that promoting cell differentiation of subcutaneous adipocyte in goats, which lays the foundation for an in-depth study of the role of CRTC2 in lipid deposition in goats. |
| format | Article |
| id | doaj-art-c69599e64f4947cebddd72c237f4977f |
| institution | DOAJ |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-c69599e64f4947cebddd72c237f4977f2025-08-20T02:56:12ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-05-0138587388310.5713/ab.24.024825370Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goatsXuening Li0Tingting Hu1Ruiwen Li2Yanyan Li3Yaqiu Lin4Yong Wang5Wei Liu6Youli Wang7 Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, China Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, ChinaObjective The aim of this study was to obtain goat CRTC2 gene sequence and elucidate its biological properties, and further study the impact of overexpression and interference of CRTC2 on the cell differentiation of goat subcutaneous precursor adipocytes. Methods The sequence of goat CRTC2 was cloned by reverse transcription (RT)-polymerase chain reaction (PCR) and its molecular characterization was analyzed. The expression of CRTC2 gene in goat tissues and subcutaneous precursor adipocytes differentiated from 0 to 120 h was examined by quantitative real-time PCR (qRT-PCR). The effects of CRTC2 on the subcutaneous precursor adipocyte differentiation were investigated by using liposome transfection, Bodipy, Oil Red O staining and qPCR. Results The results showed that the cloned goat CRTC2 gene was 2363 bp long (coding sequence [CDS] 2082 bp), encoding 693 amino acids. The relative expression levels of CRTC2 gene were highest in liver and then in kidney (p<0.05). During differentiation, the highest expression of CRTC2 in subcutaneous precursor adipocytes was observed at 120 of differentiating (p<0.01). In addition, we found that overexpression of CRTC2 significantly increased the expression of lipid metabolism-related genes (C/EBPα, C/EBPβ, PPARγ, DGAT1, DGAT2, ACC, FASN, SREBP1, AP2, LPL, ATGL) and promoted lipid accumulation. We then chemically synthesized goat CRTC2 small interfering RNA and transfected it into goat subcutaneous precursor adipocytes. The results revealed that SiRNA-mediated interference with CRTC2 significantly inhibited its differentiation and suppressed lipid droplet aggregation. Conclusion So, this study indicates that CRTC2 is a positive regulator that promoting cell differentiation of subcutaneous adipocyte in goats, which lays the foundation for an in-depth study of the role of CRTC2 in lipid deposition in goats.http://www.animbiosci.org/upload/pdf/ab-24-0248.pdfadipogenesisbiological propertycrtc2goatsubcutaneous precursor adipocytes |
| spellingShingle | Xuening Li Tingting Hu Ruiwen Li Yanyan Li Yaqiu Lin Yong Wang Wei Liu Youli Wang Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goats Animal Bioscience adipogenesis biological property crtc2 goat subcutaneous precursor adipocytes |
| title | Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goats |
| title_full | Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goats |
| title_fullStr | Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goats |
| title_full_unstemmed | Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goats |
| title_short | Effect of gene CRTC2 on the differentiation of subcutaneous precursor adipocytes in goats |
| title_sort | effect of gene crtc2 on the differentiation of subcutaneous precursor adipocytes in goats |
| topic | adipogenesis biological property crtc2 goat subcutaneous precursor adipocytes |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0248.pdf |
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