3D bioprinting for the production of a perfusable vascularized model of a cancer niche
The ever-growing need for improved in vitro models of human tissues to study both healthy and diseased states is advancing the use of techniques such as 3D Bioprinting. We here present our results on the development of a vascularized and perfusable 3D tumor mimic for studies of the early steps of Ne...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1484738/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832582976121077760 |
|---|---|
| author | Federico Maggiotto Federico Maggiotto Lorenzo Bova Lorenzo Bova Lorenzo Bova Sara Micheli Sara Micheli Camilla Pozzer Pina Fusco Pina Fusco Paolo Sgarbossa Fabrizio Billi Elisa Cimetta Elisa Cimetta |
| author_facet | Federico Maggiotto Federico Maggiotto Lorenzo Bova Lorenzo Bova Lorenzo Bova Sara Micheli Sara Micheli Camilla Pozzer Pina Fusco Pina Fusco Paolo Sgarbossa Fabrizio Billi Elisa Cimetta Elisa Cimetta |
| author_sort | Federico Maggiotto |
| collection | DOAJ |
| description | The ever-growing need for improved in vitro models of human tissues to study both healthy and diseased states is advancing the use of techniques such as 3D Bioprinting. We here present our results on the development of a vascularized and perfusable 3D tumor mimic for studies of the early steps of Neuroblastoma metastatic spread. We used a multi-material and sacrificial bioprinting approach to fabricate vascularized 3D cell-laden structures and developed a customized perfusion system enabling maintenance of growth and viability of the constructs for up to 3 weeks. Cell phenotypes and densities in co-culture for both the bulk of the construct and the endothelialization of the vascular channels were optimized to better replicate in vivo conditions and ideally simulate tumor progression. We proved the formation of an endothelium layer lining the vascular channel after 14 days of perfused culture. Cells in the bulk of the construct reflected Neuroblastoma growth and its tendency to recruit endothelial cells contributing to neovascularization. We also collected preliminary evidence of Neuroblastoma cells migration towards the vascular compartment, recapitulating the first stages of metastatic dissemination. |
| format | Article |
| id | doaj-art-c694d02236904f6e9ce511b67f98975a |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-c694d02236904f6e9ce511b67f98975a2025-01-29T06:45:40ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011310.3389/fbioe.2025.148473814847383D bioprinting for the production of a perfusable vascularized model of a cancer nicheFederico Maggiotto0Federico Maggiotto1Lorenzo Bova2Lorenzo Bova3Lorenzo Bova4Sara Micheli5Sara Micheli6Camilla Pozzer7Pina Fusco8Pina Fusco9Paolo Sgarbossa10Fabrizio Billi11Elisa Cimetta12Elisa Cimetta13Department of Industrial Engineering (DII), University of Padua, Padova, ItalyFondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP), Padova, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova, ItalyFondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP), Padova, ItalyUCLA Department of Orthopaedic Surgery, David Geffen School of Medicine, Los Angeles, CA, United StatesDepartment of Industrial Engineering (DII), University of Padua, Padova, ItalyFondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP), Padova, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova, ItalyFondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP), Padova, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova, ItalyUCLA Department of Orthopaedic Surgery, David Geffen School of Medicine, Los Angeles, CA, United StatesDepartment of Industrial Engineering (DII), University of Padua, Padova, ItalyFondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP), Padova, ItalyThe ever-growing need for improved in vitro models of human tissues to study both healthy and diseased states is advancing the use of techniques such as 3D Bioprinting. We here present our results on the development of a vascularized and perfusable 3D tumor mimic for studies of the early steps of Neuroblastoma metastatic spread. We used a multi-material and sacrificial bioprinting approach to fabricate vascularized 3D cell-laden structures and developed a customized perfusion system enabling maintenance of growth and viability of the constructs for up to 3 weeks. Cell phenotypes and densities in co-culture for both the bulk of the construct and the endothelialization of the vascular channels were optimized to better replicate in vivo conditions and ideally simulate tumor progression. We proved the formation of an endothelium layer lining the vascular channel after 14 days of perfused culture. Cells in the bulk of the construct reflected Neuroblastoma growth and its tendency to recruit endothelial cells contributing to neovascularization. We also collected preliminary evidence of Neuroblastoma cells migration towards the vascular compartment, recapitulating the first stages of metastatic dissemination.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1484738/full3D bioprintingvascularizationperfusioncancer nichemetastasisneuroblastoma |
| spellingShingle | Federico Maggiotto Federico Maggiotto Lorenzo Bova Lorenzo Bova Lorenzo Bova Sara Micheli Sara Micheli Camilla Pozzer Pina Fusco Pina Fusco Paolo Sgarbossa Fabrizio Billi Elisa Cimetta Elisa Cimetta 3D bioprinting for the production of a perfusable vascularized model of a cancer niche Frontiers in Bioengineering and Biotechnology 3D bioprinting vascularization perfusion cancer niche metastasis neuroblastoma |
| title | 3D bioprinting for the production of a perfusable vascularized model of a cancer niche |
| title_full | 3D bioprinting for the production of a perfusable vascularized model of a cancer niche |
| title_fullStr | 3D bioprinting for the production of a perfusable vascularized model of a cancer niche |
| title_full_unstemmed | 3D bioprinting for the production of a perfusable vascularized model of a cancer niche |
| title_short | 3D bioprinting for the production of a perfusable vascularized model of a cancer niche |
| title_sort | 3d bioprinting for the production of a perfusable vascularized model of a cancer niche |
| topic | 3D bioprinting vascularization perfusion cancer niche metastasis neuroblastoma |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1484738/full |
| work_keys_str_mv | AT federicomaggiotto 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT federicomaggiotto 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT lorenzobova 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT lorenzobova 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT lorenzobova 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT saramicheli 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT saramicheli 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT camillapozzer 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT pinafusco 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT pinafusco 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT paolosgarbossa 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT fabriziobilli 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT elisacimetta 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche AT elisacimetta 3dbioprintingfortheproductionofaperfusablevascularizedmodelofacancerniche |