Synthesis and characterization of N-octanoyl glycol chitosan as a novel temperature and pH-sensitive injectable hydrogel for biomedical applications
Multi-responsive hydrogel formulations have received significant attention in biomedical applications as promising injectable depots and platforms for tissue regeneration. In this study, a new pH- and temperature-sensitive polymer, octanoyl glycol chitosan (OGC), synthesized by controlled N-octanoyl...
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Elsevier
2025-06-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127525004721 |
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| author | Hoang-Minh Pham Chanyang Joo Mst Jannatul Ferdous Israr Ali Sun-Woong Kang Kang Moo Huh |
| author_facet | Hoang-Minh Pham Chanyang Joo Mst Jannatul Ferdous Israr Ali Sun-Woong Kang Kang Moo Huh |
| author_sort | Hoang-Minh Pham |
| collection | DOAJ |
| description | Multi-responsive hydrogel formulations have received significant attention in biomedical applications as promising injectable depots and platforms for tissue regeneration. In this study, a new pH- and temperature-sensitive polymer, octanoyl glycol chitosan (OGC), synthesized by controlled N-octanoylation of glycol chitosan and evaluated as a reversible hydrogel system capable of undergoing sol–gel transition in response to changes in pH and temperature. The OGC hydrogels, with varying degrees of octanoylation (DO), were characterized by 1H NMR and ATR-FTIR analyses, and their physicochemical properties were evaluated in terms of injectability, thermo- and pH-sensitivity, biodegradability, self-healing, cytotoxicity, 3D cell culture support, and in vivo biocompatibility. The OGC exhibited controlled thermosensitive sol–gel transition behavior, with transition temperature ranging from 23 °C to 58 °C, which could be tuned by adjusting the DO and polymer concentration. The effect of pH on gelation was also observed within the pH range of 5 to 7.4. OGC demonstrated excellent injectability and self-healing properties. In scaffold-free 3D cell culture systems, the OGC supported spheroid formation and maintained stability for up to 7 days. In vivo studies in ICR mice revealed no significant inflammatory response upon implantation. These results suggest that the OGC could be a promising injectable biomaterial for various biomedical applications. |
| format | Article |
| id | doaj-art-c68956d75f544a22bf64c6ac89980b99 |
| institution | OA Journals |
| issn | 0264-1275 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Materials & Design |
| spelling | doaj-art-c68956d75f544a22bf64c6ac89980b992025-08-20T02:09:18ZengElsevierMaterials & Design0264-12752025-06-0125411405210.1016/j.matdes.2025.114052Synthesis and characterization of N-octanoyl glycol chitosan as a novel temperature and pH-sensitive injectable hydrogel for biomedical applicationsHoang-Minh Pham0Chanyang Joo1Mst Jannatul Ferdous2Israr Ali3Sun-Woong Kang4Kang Moo Huh5Department of Materials Science and Engineering, Chungnam National University, Daejeon 34134, Republic of KoreaDepartment of Polymer Science and Engineering, Chungnam National University, Daejeon 34134, Republic of KoreaDepartment of Materials Science and Engineering, Chungnam National University, Daejeon 34134, Republic of KoreaDepartment of Materials Science and Engineering, Chungnam National University, Daejeon 34134, Republic of KoreaResearch Group for Biomimetic Advanced Technology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, Republic of Korea; School of Korea Institute of Toxicology, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea; Corresponding author at: Research Group for Biomimetic Advanced Technology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, Republic of Korea.Department of Materials Science and Engineering, Chungnam National University, Daejeon 34134, Republic of Korea; Department of Polymer Science and Engineering, Chungnam National University, Daejeon 34134, Republic of Korea; Corresponding author at: Department of Polymer Science and Engineering, Chungnam National University, Daejeon 34134, Republic of Korea.Multi-responsive hydrogel formulations have received significant attention in biomedical applications as promising injectable depots and platforms for tissue regeneration. In this study, a new pH- and temperature-sensitive polymer, octanoyl glycol chitosan (OGC), synthesized by controlled N-octanoylation of glycol chitosan and evaluated as a reversible hydrogel system capable of undergoing sol–gel transition in response to changes in pH and temperature. The OGC hydrogels, with varying degrees of octanoylation (DO), were characterized by 1H NMR and ATR-FTIR analyses, and their physicochemical properties were evaluated in terms of injectability, thermo- and pH-sensitivity, biodegradability, self-healing, cytotoxicity, 3D cell culture support, and in vivo biocompatibility. The OGC exhibited controlled thermosensitive sol–gel transition behavior, with transition temperature ranging from 23 °C to 58 °C, which could be tuned by adjusting the DO and polymer concentration. The effect of pH on gelation was also observed within the pH range of 5 to 7.4. OGC demonstrated excellent injectability and self-healing properties. In scaffold-free 3D cell culture systems, the OGC supported spheroid formation and maintained stability for up to 7 days. In vivo studies in ICR mice revealed no significant inflammatory response upon implantation. These results suggest that the OGC could be a promising injectable biomaterial for various biomedical applications.http://www.sciencedirect.com/science/article/pii/S0264127525004721N-octanoyl glycol chitosanpH-sensitive hydrogelThermosensitive hydrogelDual-sensitive hydrogelSpheroid culture |
| spellingShingle | Hoang-Minh Pham Chanyang Joo Mst Jannatul Ferdous Israr Ali Sun-Woong Kang Kang Moo Huh Synthesis and characterization of N-octanoyl glycol chitosan as a novel temperature and pH-sensitive injectable hydrogel for biomedical applications Materials & Design N-octanoyl glycol chitosan pH-sensitive hydrogel Thermosensitive hydrogel Dual-sensitive hydrogel Spheroid culture |
| title | Synthesis and characterization of N-octanoyl glycol chitosan as a novel temperature and pH-sensitive injectable hydrogel for biomedical applications |
| title_full | Synthesis and characterization of N-octanoyl glycol chitosan as a novel temperature and pH-sensitive injectable hydrogel for biomedical applications |
| title_fullStr | Synthesis and characterization of N-octanoyl glycol chitosan as a novel temperature and pH-sensitive injectable hydrogel for biomedical applications |
| title_full_unstemmed | Synthesis and characterization of N-octanoyl glycol chitosan as a novel temperature and pH-sensitive injectable hydrogel for biomedical applications |
| title_short | Synthesis and characterization of N-octanoyl glycol chitosan as a novel temperature and pH-sensitive injectable hydrogel for biomedical applications |
| title_sort | synthesis and characterization of n octanoyl glycol chitosan as a novel temperature and ph sensitive injectable hydrogel for biomedical applications |
| topic | N-octanoyl glycol chitosan pH-sensitive hydrogel Thermosensitive hydrogel Dual-sensitive hydrogel Spheroid culture |
| url | http://www.sciencedirect.com/science/article/pii/S0264127525004721 |
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