ISOLATION AND IDENTIFICATION OF COLIPHAGE FROM UNTREATED WASTEWATER IN KUALA PILAH NEGERI SEMBILAN
Bacteriophages are viruses that can only infect bacterial cells and are perceptible in almost all places where living bacteria are found, as they are highly host specific. Wastewater comprises microorganisms that have become contaminated by the environment. These microorganisms, particularly bacteri...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universiti Teknologi MARA, Negeri Sembilan
2025-04-01
|
| Series: | Journal of Academia |
| Subjects: | |
| Online Access: | https://journal.uitm.edu.my/ojs/index.php/JOA/article/view/4558 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850135412315521024 |
|---|---|
| author | Muhammad Hafizhullah Anuar Nur Surya Zulkifly Nurhamimah Zainal Abidin Sharifah Aminah Syed Mohamad Nur Intan Hasbullah Rashidah Iberahim |
| author_facet | Muhammad Hafizhullah Anuar Nur Surya Zulkifly Nurhamimah Zainal Abidin Sharifah Aminah Syed Mohamad Nur Intan Hasbullah Rashidah Iberahim |
| author_sort | Muhammad Hafizhullah Anuar |
| collection | DOAJ |
| description | Bacteriophages are viruses that can only infect bacterial cells and are perceptible in almost all places where living bacteria are found, as they are highly host specific. Wastewater comprises microorganisms that have become contaminated by the environment. These microorganisms, particularly bacteria, may develop antibiotic resistance and constitute a serious threat to human health. Therefore, the goal of this research is to isolate coliphage from untreated wastewater and to identify the susceptible coliform host. The method used for bacteriophage isolation is plaque formation assay and the selected plaque was further enriched and purified using the double-layer plaque technique to classify bacteriophages. Identification of susceptible coliform host using gram stain and biochemical analysis results were compared with other coliform bacteria (Klebsiella sp, Escherichia coli, and Shigella sp.) to identify the susceptible coliphage host. The isolated coliphages appear late from 2 out of 3 samples collected around Kuala Pilah town. The plaque shows were small and turbid after 7 days of incubation in 37°C. It was suspected to be a temperate phage as the plaque developed was turbid. The susceptible host shows similar biochemical characteristics as Klebsiella sp. In conclusion, the bacteriophage obtained from this study is a temperate bacteriophage from environmental wastewater that could infect a coliphage likeKlebsiella sp. Further identification of susceptible host was suggested including determination of its medical usage. |
| format | Article |
| id | doaj-art-c686df6284a54511b0fcad777e76d059 |
| institution | OA Journals |
| issn | 2289-6368 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Universiti Teknologi MARA, Negeri Sembilan |
| record_format | Article |
| series | Journal of Academia |
| spelling | doaj-art-c686df6284a54511b0fcad777e76d0592025-08-20T02:31:26ZengUniversiti Teknologi MARA, Negeri SembilanJournal of Academia2289-63682025-04-01131293310.24191/joa.v13i1.4558ISOLATION AND IDENTIFICATION OF COLIPHAGE FROM UNTREATED WASTEWATER IN KUALA PILAH NEGERI SEMBILANMuhammad Hafizhullah Anuar Nur Surya Zulkifly 0Nurhamimah Zainal Abidin 1Sharifah Aminah Syed Mohamad 2Nur Intan Hasbullah 3Rashidah Iberahim4School of Biological Sciences, Faculty of Applied SciencesUniversiti Teknologi MARA (UiTM), Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, MalaysiaSchool of Biological Sciences, Faculty of Applied SciencesUniversiti Teknologi MARA (UiTM), Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, MalaysiaMicrobial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), UniversitiTeknologi MARA (UiTM), Puncak Alam, Selangor, MalaysiaSchool of Biological Sciences, Faculty of Applied SciencesUniversiti Teknologi MARA (UiTM), Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, MalaysiaSchool of Biological Sciences, Faculty of Applied SciencesUniversiti Teknologi MARA (UiTM), Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, MalaysiaBacteriophages are viruses that can only infect bacterial cells and are perceptible in almost all places where living bacteria are found, as they are highly host specific. Wastewater comprises microorganisms that have become contaminated by the environment. These microorganisms, particularly bacteria, may develop antibiotic resistance and constitute a serious threat to human health. Therefore, the goal of this research is to isolate coliphage from untreated wastewater and to identify the susceptible coliform host. The method used for bacteriophage isolation is plaque formation assay and the selected plaque was further enriched and purified using the double-layer plaque technique to classify bacteriophages. Identification of susceptible coliform host using gram stain and biochemical analysis results were compared with other coliform bacteria (Klebsiella sp, Escherichia coli, and Shigella sp.) to identify the susceptible coliphage host. The isolated coliphages appear late from 2 out of 3 samples collected around Kuala Pilah town. The plaque shows were small and turbid after 7 days of incubation in 37°C. It was suspected to be a temperate phage as the plaque developed was turbid. The susceptible host shows similar biochemical characteristics as Klebsiella sp. In conclusion, the bacteriophage obtained from this study is a temperate bacteriophage from environmental wastewater that could infect a coliphage likeKlebsiella sp. Further identification of susceptible host was suggested including determination of its medical usage.https://journal.uitm.edu.my/ojs/index.php/JOA/article/view/4558bacteriophagewastewatere. coliplaque |
| spellingShingle | Muhammad Hafizhullah Anuar Nur Surya Zulkifly Nurhamimah Zainal Abidin Sharifah Aminah Syed Mohamad Nur Intan Hasbullah Rashidah Iberahim ISOLATION AND IDENTIFICATION OF COLIPHAGE FROM UNTREATED WASTEWATER IN KUALA PILAH NEGERI SEMBILAN Journal of Academia bacteriophage wastewater e. coli plaque |
| title | ISOLATION AND IDENTIFICATION OF COLIPHAGE FROM UNTREATED WASTEWATER IN KUALA PILAH NEGERI SEMBILAN |
| title_full | ISOLATION AND IDENTIFICATION OF COLIPHAGE FROM UNTREATED WASTEWATER IN KUALA PILAH NEGERI SEMBILAN |
| title_fullStr | ISOLATION AND IDENTIFICATION OF COLIPHAGE FROM UNTREATED WASTEWATER IN KUALA PILAH NEGERI SEMBILAN |
| title_full_unstemmed | ISOLATION AND IDENTIFICATION OF COLIPHAGE FROM UNTREATED WASTEWATER IN KUALA PILAH NEGERI SEMBILAN |
| title_short | ISOLATION AND IDENTIFICATION OF COLIPHAGE FROM UNTREATED WASTEWATER IN KUALA PILAH NEGERI SEMBILAN |
| title_sort | isolation and identification of coliphage from untreated wastewater in kuala pilah negeri sembilan |
| topic | bacteriophage wastewater e. coli plaque |
| url | https://journal.uitm.edu.my/ojs/index.php/JOA/article/view/4558 |
| work_keys_str_mv | AT muhammadhafizhullahanuar isolationandidentificationofcoliphagefromuntreatedwastewaterinkualapilahnegerisembilan AT nursuryazulkifly isolationandidentificationofcoliphagefromuntreatedwastewaterinkualapilahnegerisembilan AT nurhamimahzainalabidin isolationandidentificationofcoliphagefromuntreatedwastewaterinkualapilahnegerisembilan AT sharifahaminahsyedmohamad isolationandidentificationofcoliphagefromuntreatedwastewaterinkualapilahnegerisembilan AT nurintanhasbullah isolationandidentificationofcoliphagefromuntreatedwastewaterinkualapilahnegerisembilan AT rashidahiberahim isolationandidentificationofcoliphagefromuntreatedwastewaterinkualapilahnegerisembilan |