Continuous Electrical Current and Zinc Sulphate Administered by Transdermal Iontophoresis Improves Skin Healing in Diabetic Rats Induced by Alloxan: Morphological and Ultrastructural Analysis
Purpose. Evaluated the effects of continuous electrical current (CEC) or zinc administrated by transdermal iontophoresis (Zn+TDI). Methods. 120 male Wistar rats were submitted to an incision surgery at the anterior region of abdomen and distributed into 6 experimental groups with 40 animals: 3 diabe...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2014/980232 |
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| author | Lucas Langoni Cassettari Pedro Colli Rocha Dias Amanda Natália Lucchesi Maurício Ferraz de Arruda Érika Veruska Paiva Ortolan Mariângela Esther A. Marques César Tadeu Spadella |
| author_facet | Lucas Langoni Cassettari Pedro Colli Rocha Dias Amanda Natália Lucchesi Maurício Ferraz de Arruda Érika Veruska Paiva Ortolan Mariângela Esther A. Marques César Tadeu Spadella |
| author_sort | Lucas Langoni Cassettari |
| collection | DOAJ |
| description | Purpose. Evaluated the effects of continuous electrical current (CEC) or zinc administrated by transdermal iontophoresis (Zn+TDI). Methods. 120 male Wistar rats were submitted to an incision surgery at the anterior region of abdomen and distributed into 6 experimental groups with 40 animals: 3 diabetic groups and 3 normal groups, untreated and treated with CEC alone or with Zn + TDI. Each group was further divided into 4 subgroups with 10 rats each to be evaluated on the 4th, 7th, 14th, and 21st day after surgery. In each period, clinical and laboratory parameters from the animals were analyzed. Results. The analysis by optical and scanning electron microscopy showed a delay in the phases of wound healing in diabetic rats without treatment in all periods of the experiment; breaking strength (BS) was significantly reduced in skin scars of untreated diabetic rats when compared to other groups. In contrast, BS in skin scars of nondiabetic groups and diabetic rats treated with Zn + TDI showed significant increase in those, besides not presenting delayed healing. Conclusion. Electrical stimulation of surgical wounds used alone or in association with zinc by TDI is able to consistently improve the morphological and ultrastructural changes observed in the healing of diabetic animals. |
| format | Article |
| id | doaj-art-c686a47ca61145138bd6f048cad0c014 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-c686a47ca61145138bd6f048cad0c0142025-02-03T05:59:25ZengWileyJournal of Diabetes Research2314-67452314-67532014-01-01201410.1155/2014/980232980232Continuous Electrical Current and Zinc Sulphate Administered by Transdermal Iontophoresis Improves Skin Healing in Diabetic Rats Induced by Alloxan: Morphological and Ultrastructural AnalysisLucas Langoni Cassettari0Pedro Colli Rocha Dias1Amanda Natália Lucchesi2Maurício Ferraz de Arruda3Érika Veruska Paiva Ortolan4Mariângela Esther A. Marques5César Tadeu Spadella6Faculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, BrazilFaculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, BrazilGraduate Program in General Basis of Surgery, Faculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, BrazilMunicipal Institute of Higher Education, IMES-FAFICA, 55030-902 Catanduva, SP, BrazilDepartment of Surgery and Orthopedics, Faculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, BrazilDepartment of Pathology, Faculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, BrazilDepartment of Surgery and Orthopedics, Faculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, BrazilPurpose. Evaluated the effects of continuous electrical current (CEC) or zinc administrated by transdermal iontophoresis (Zn+TDI). Methods. 120 male Wistar rats were submitted to an incision surgery at the anterior region of abdomen and distributed into 6 experimental groups with 40 animals: 3 diabetic groups and 3 normal groups, untreated and treated with CEC alone or with Zn + TDI. Each group was further divided into 4 subgroups with 10 rats each to be evaluated on the 4th, 7th, 14th, and 21st day after surgery. In each period, clinical and laboratory parameters from the animals were analyzed. Results. The analysis by optical and scanning electron microscopy showed a delay in the phases of wound healing in diabetic rats without treatment in all periods of the experiment; breaking strength (BS) was significantly reduced in skin scars of untreated diabetic rats when compared to other groups. In contrast, BS in skin scars of nondiabetic groups and diabetic rats treated with Zn + TDI showed significant increase in those, besides not presenting delayed healing. Conclusion. Electrical stimulation of surgical wounds used alone or in association with zinc by TDI is able to consistently improve the morphological and ultrastructural changes observed in the healing of diabetic animals.http://dx.doi.org/10.1155/2014/980232 |
| spellingShingle | Lucas Langoni Cassettari Pedro Colli Rocha Dias Amanda Natália Lucchesi Maurício Ferraz de Arruda Érika Veruska Paiva Ortolan Mariângela Esther A. Marques César Tadeu Spadella Continuous Electrical Current and Zinc Sulphate Administered by Transdermal Iontophoresis Improves Skin Healing in Diabetic Rats Induced by Alloxan: Morphological and Ultrastructural Analysis Journal of Diabetes Research |
| title | Continuous Electrical Current and Zinc Sulphate Administered by Transdermal Iontophoresis Improves Skin Healing in Diabetic Rats Induced by Alloxan: Morphological and Ultrastructural Analysis |
| title_full | Continuous Electrical Current and Zinc Sulphate Administered by Transdermal Iontophoresis Improves Skin Healing in Diabetic Rats Induced by Alloxan: Morphological and Ultrastructural Analysis |
| title_fullStr | Continuous Electrical Current and Zinc Sulphate Administered by Transdermal Iontophoresis Improves Skin Healing in Diabetic Rats Induced by Alloxan: Morphological and Ultrastructural Analysis |
| title_full_unstemmed | Continuous Electrical Current and Zinc Sulphate Administered by Transdermal Iontophoresis Improves Skin Healing in Diabetic Rats Induced by Alloxan: Morphological and Ultrastructural Analysis |
| title_short | Continuous Electrical Current and Zinc Sulphate Administered by Transdermal Iontophoresis Improves Skin Healing in Diabetic Rats Induced by Alloxan: Morphological and Ultrastructural Analysis |
| title_sort | continuous electrical current and zinc sulphate administered by transdermal iontophoresis improves skin healing in diabetic rats induced by alloxan morphological and ultrastructural analysis |
| url | http://dx.doi.org/10.1155/2014/980232 |
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