Identification and Preliminary Analysis of Granulosa Cell Biomarkers to Predict Oocyte In Vitro Maturation Outcome in the Southern White Rhinoceros (<i>Ceratotherium simum simum</i>)
In recent years, biomarkers in granulosa cells (GC) have been determined and associated in several species with oocyte maturation, in vitro fertilization success, and embryo development outcomes. The identification of biomarkers of oocyte competence can aid in improving assisted reproductive technol...
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2024-12-01
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| author | Elena Ruggeri Kristin Klohonatz Barbara Durrant Marc-André Sirard |
| author_facet | Elena Ruggeri Kristin Klohonatz Barbara Durrant Marc-André Sirard |
| author_sort | Elena Ruggeri |
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| description | In recent years, biomarkers in granulosa cells (GC) have been determined and associated in several species with oocyte maturation, in vitro fertilization success, and embryo development outcomes. The identification of biomarkers of oocyte competence can aid in improving assisted reproductive technologies (ARTs) in the southern white rhino (SWR). This study aimed to identify biomarkers present in SWR GC associated with oocytes that either did or did not mature in vitro. We evaluated follicle development (FD), meiotic competence (MC), cell death and atresia (CDA), and embryonic genome activation (EGA). Our objective was to design biomarkers to predict oocyte in vitro maturation results in the SWR. RNA was isolated from GC obtained during ovum pick up (OPU) for qPCR analysis. Overall, 22 genes were assessed, and nine were differentially expressed between GC from oocytes that did or did not mature in vitro (FD-GDF9 and mTOR; MC-GGPS1, JMY, and NPR2; CDA-COL4A1, MACIR, and TMPO; EGA-NFYA). From these data, we determined that GC can be used as a predictor for oocyte in vitro maturation outcome in the SWR. Our results provide crucial information needed to improve in vitro maturation and ARTs in this species. |
| format | Article |
| id | doaj-art-c67c625f1bfa497db90553f0c5cf9d71 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-c67c625f1bfa497db90553f0c5cf9d712024-12-13T16:21:36ZengMDPI AGAnimals2076-26152024-12-011423353810.3390/ani14233538Identification and Preliminary Analysis of Granulosa Cell Biomarkers to Predict Oocyte In Vitro Maturation Outcome in the Southern White Rhinoceros (<i>Ceratotherium simum simum</i>)Elena Ruggeri0Kristin Klohonatz1Barbara Durrant2Marc-André Sirard3Reproductive Sciences, Conservation Science Wildlife Health, San Diego Zoo Wildlife Alliance, Escondido, CA 92027, USACenter for Research on Reproduction and Women’s Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USAReproductive Sciences, Conservation Science Wildlife Health, San Diego Zoo Wildlife Alliance, Escondido, CA 92027, USADépartement des Sciences Animales, Université Laval, Quebec City, QC G1V 0A6, CanadaIn recent years, biomarkers in granulosa cells (GC) have been determined and associated in several species with oocyte maturation, in vitro fertilization success, and embryo development outcomes. The identification of biomarkers of oocyte competence can aid in improving assisted reproductive technologies (ARTs) in the southern white rhino (SWR). This study aimed to identify biomarkers present in SWR GC associated with oocytes that either did or did not mature in vitro. We evaluated follicle development (FD), meiotic competence (MC), cell death and atresia (CDA), and embryonic genome activation (EGA). Our objective was to design biomarkers to predict oocyte in vitro maturation results in the SWR. RNA was isolated from GC obtained during ovum pick up (OPU) for qPCR analysis. Overall, 22 genes were assessed, and nine were differentially expressed between GC from oocytes that did or did not mature in vitro (FD-GDF9 and mTOR; MC-GGPS1, JMY, and NPR2; CDA-COL4A1, MACIR, and TMPO; EGA-NFYA). From these data, we determined that GC can be used as a predictor for oocyte in vitro maturation outcome in the SWR. Our results provide crucial information needed to improve in vitro maturation and ARTs in this species.https://www.mdpi.com/2076-2615/14/23/3538wildlifeassisted reproductive technologiesoocytes |
| spellingShingle | Elena Ruggeri Kristin Klohonatz Barbara Durrant Marc-André Sirard Identification and Preliminary Analysis of Granulosa Cell Biomarkers to Predict Oocyte In Vitro Maturation Outcome in the Southern White Rhinoceros (<i>Ceratotherium simum simum</i>) Animals wildlife assisted reproductive technologies oocytes |
| title | Identification and Preliminary Analysis of Granulosa Cell Biomarkers to Predict Oocyte In Vitro Maturation Outcome in the Southern White Rhinoceros (<i>Ceratotherium simum simum</i>) |
| title_full | Identification and Preliminary Analysis of Granulosa Cell Biomarkers to Predict Oocyte In Vitro Maturation Outcome in the Southern White Rhinoceros (<i>Ceratotherium simum simum</i>) |
| title_fullStr | Identification and Preliminary Analysis of Granulosa Cell Biomarkers to Predict Oocyte In Vitro Maturation Outcome in the Southern White Rhinoceros (<i>Ceratotherium simum simum</i>) |
| title_full_unstemmed | Identification and Preliminary Analysis of Granulosa Cell Biomarkers to Predict Oocyte In Vitro Maturation Outcome in the Southern White Rhinoceros (<i>Ceratotherium simum simum</i>) |
| title_short | Identification and Preliminary Analysis of Granulosa Cell Biomarkers to Predict Oocyte In Vitro Maturation Outcome in the Southern White Rhinoceros (<i>Ceratotherium simum simum</i>) |
| title_sort | identification and preliminary analysis of granulosa cell biomarkers to predict oocyte in vitro maturation outcome in the southern white rhinoceros i ceratotherium simum simum i |
| topic | wildlife assisted reproductive technologies oocytes |
| url | https://www.mdpi.com/2076-2615/14/23/3538 |
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