Comprehensive analysis of the physiological and molecular responses of phosphate-solubilizing bacterium Burkholderia gladioli DJB4–8 in promoting maize growth
Phosphorus (P) is one of the essential macroelements for the growth of maize. The deficiency of P in maize will result in adverse effects, including chlorosis and reduced yield. The Hexi Corridor in China serves as the principal region for seed maize production, with chemical phosphate fertilizer re...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1611674/full |
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| author | Dao-Jun Guo Dao-Jun Guo Dao-Jun Guo Guo-Rong Yang Pratiksha Singh Juan-Juan Wang Juan-Juan Wang Xue-Mei Lan Rajesh Kumar Singh Jing Guo Yu-Die Dong Dong-Ping Li Dong-Ping Li Dong-Ping Li Bin Yang Bin Yang |
| author_facet | Dao-Jun Guo Dao-Jun Guo Dao-Jun Guo Guo-Rong Yang Pratiksha Singh Juan-Juan Wang Juan-Juan Wang Xue-Mei Lan Rajesh Kumar Singh Jing Guo Yu-Die Dong Dong-Ping Li Dong-Ping Li Dong-Ping Li Bin Yang Bin Yang |
| author_sort | Dao-Jun Guo |
| collection | DOAJ |
| description | Phosphorus (P) is one of the essential macroelements for the growth of maize. The deficiency of P in maize will result in adverse effects, including chlorosis and reduced yield. The Hexi Corridor in China serves as the principal region for seed maize production, with chemical phosphate fertilizer remaining the predominant source of P delivery for local maize cultivation. Nonetheless, the agricultural non-point source pollution resulting from the prolonged application of artificial phosphate fertilizers is intensifying. P in farmland soil often exists in an insoluble form, which plants cannot directly absorb and utilize. Phosphate-solubilizing bacteria (PSB) in the rhizosphere are a kind of plant growth-promoting rhizobacteria (PGPR) that can transform insoluble P in soil into soluble P for plants to absorb and utilize. Utilizing PGPR in agricultural production is an ecological approach to achieving sustainable development in agricultural practices and output. In this study, 41 strains of bacteria were isolated from the rhizosphere soil of four maize varieties. According to an in vitro plant growth-promoting (PGP) feature study and 16S RNA molecular identification, Burkholderia gladioli DJB4-8, among all strains tested, exhibited the highest in vitro PGP activity, with a phosphate-solubilizing ability of 8.99 mg/L. By scanning electron microscope (SEM) and green fluorescent protein (GFP) labeling technique, it was found that strain DJB4–8 formed a colonization symbiotic system with maize roots. The inoculation of maize Zhengdan 958 with strain DJB4–8 altered the plant’s photosynthetic physiology and indole-3-acetic acid (IAA) level, and it also dramatically increased the plant’s growth rate. The combined analysis of transcriptome and metabolomics showed that the key genes and metabolites in the interaction between strain DJB4–8 and maize were mainly concentrated in plant growth key pathways such as plant hormone signal transduction, phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, phenylpropane biosynthesis, pentose phosphate pathway, zeatin biosynthesis, amino sugar and nucleotide sugar metabolism, and glutathione metabolism. These findings shed light on the need for additional research into the mechanism of interaction between PSB and maize. |
| format | Article |
| id | doaj-art-c67ae3d66d9747708120717bb43c47ff |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-c67ae3d66d9747708120717bb43c47ff2025-08-20T03:46:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-06-011610.3389/fpls.2025.16116741611674Comprehensive analysis of the physiological and molecular responses of phosphate-solubilizing bacterium Burkholderia gladioli DJB4–8 in promoting maize growthDao-Jun Guo0Dao-Jun Guo1Dao-Jun Guo2Guo-Rong Yang3Pratiksha Singh4Juan-Juan Wang5Juan-Juan Wang6Xue-Mei Lan7Rajesh Kumar Singh8Jing Guo9Yu-Die Dong10Dong-Ping Li11Dong-Ping Li12Dong-Ping Li13Bin Yang14Bin Yang15Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye, Gansu, ChinaCollege of Life Sciences and Engineering, Hexi University, Zhangye, Gansu, ChinaGuangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, Sichuan, ChinaGuangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaKey Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye, Gansu, ChinaCollege of Life Sciences and Engineering, Hexi University, Zhangye, Gansu, ChinaCollege of Agriculture and Ecological Engineering, Hexi University, Zhangye, Gansu, ChinaGuangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaCollege of Life Sciences and Engineering, Hexi University, Zhangye, Gansu, ChinaCollege of Life Sciences and Engineering, Hexi University, Zhangye, Gansu, ChinaKey Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye, Gansu, ChinaCollege of Life Sciences and Engineering, Hexi University, Zhangye, Gansu, ChinaGuangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaKey Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye, Gansu, ChinaCollege of Life Sciences and Engineering, Hexi University, Zhangye, Gansu, ChinaPhosphorus (P) is one of the essential macroelements for the growth of maize. The deficiency of P in maize will result in adverse effects, including chlorosis and reduced yield. The Hexi Corridor in China serves as the principal region for seed maize production, with chemical phosphate fertilizer remaining the predominant source of P delivery for local maize cultivation. Nonetheless, the agricultural non-point source pollution resulting from the prolonged application of artificial phosphate fertilizers is intensifying. P in farmland soil often exists in an insoluble form, which plants cannot directly absorb and utilize. Phosphate-solubilizing bacteria (PSB) in the rhizosphere are a kind of plant growth-promoting rhizobacteria (PGPR) that can transform insoluble P in soil into soluble P for plants to absorb and utilize. Utilizing PGPR in agricultural production is an ecological approach to achieving sustainable development in agricultural practices and output. In this study, 41 strains of bacteria were isolated from the rhizosphere soil of four maize varieties. According to an in vitro plant growth-promoting (PGP) feature study and 16S RNA molecular identification, Burkholderia gladioli DJB4-8, among all strains tested, exhibited the highest in vitro PGP activity, with a phosphate-solubilizing ability of 8.99 mg/L. By scanning electron microscope (SEM) and green fluorescent protein (GFP) labeling technique, it was found that strain DJB4–8 formed a colonization symbiotic system with maize roots. The inoculation of maize Zhengdan 958 with strain DJB4–8 altered the plant’s photosynthetic physiology and indole-3-acetic acid (IAA) level, and it also dramatically increased the plant’s growth rate. The combined analysis of transcriptome and metabolomics showed that the key genes and metabolites in the interaction between strain DJB4–8 and maize were mainly concentrated in plant growth key pathways such as plant hormone signal transduction, phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, phenylpropane biosynthesis, pentose phosphate pathway, zeatin biosynthesis, amino sugar and nucleotide sugar metabolism, and glutathione metabolism. These findings shed light on the need for additional research into the mechanism of interaction between PSB and maize.https://www.frontiersin.org/articles/10.3389/fpls.2025.1611674/fullBurkholderia gladioli DJB4-8phosphate-solubilizing bacteriaPGPmaizetranscriptomemetabolomics |
| spellingShingle | Dao-Jun Guo Dao-Jun Guo Dao-Jun Guo Guo-Rong Yang Pratiksha Singh Juan-Juan Wang Juan-Juan Wang Xue-Mei Lan Rajesh Kumar Singh Jing Guo Yu-Die Dong Dong-Ping Li Dong-Ping Li Dong-Ping Li Bin Yang Bin Yang Comprehensive analysis of the physiological and molecular responses of phosphate-solubilizing bacterium Burkholderia gladioli DJB4–8 in promoting maize growth Frontiers in Plant Science Burkholderia gladioli DJB4-8 phosphate-solubilizing bacteria PGP maize transcriptome metabolomics |
| title | Comprehensive analysis of the physiological and molecular responses of phosphate-solubilizing bacterium Burkholderia gladioli DJB4–8 in promoting maize growth |
| title_full | Comprehensive analysis of the physiological and molecular responses of phosphate-solubilizing bacterium Burkholderia gladioli DJB4–8 in promoting maize growth |
| title_fullStr | Comprehensive analysis of the physiological and molecular responses of phosphate-solubilizing bacterium Burkholderia gladioli DJB4–8 in promoting maize growth |
| title_full_unstemmed | Comprehensive analysis of the physiological and molecular responses of phosphate-solubilizing bacterium Burkholderia gladioli DJB4–8 in promoting maize growth |
| title_short | Comprehensive analysis of the physiological and molecular responses of phosphate-solubilizing bacterium Burkholderia gladioli DJB4–8 in promoting maize growth |
| title_sort | comprehensive analysis of the physiological and molecular responses of phosphate solubilizing bacterium burkholderia gladioli djb4 8 in promoting maize growth |
| topic | Burkholderia gladioli DJB4-8 phosphate-solubilizing bacteria PGP maize transcriptome metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1611674/full |
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