Rapid Microwave-Assisted Synthesis of CuSe Nanoparticles for High-Sensitivity Serotonin Biosensing in Serum

In this study, a simple and effective approach was developed for the quantitative detection of serotonin. Hexagonal copper selenide nanostructures (CuSe) were employed to modify a disposable screen-printed carbon electrode (SPCE), and their ability to electrochemically detect serotonin in serum samp...

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Main Authors: Sankar Sekar, Ramalingam Manikandan, Shiva Kumar Arumugasamy, Saravanan Sekar, Youngmin Lee, Seung-Cheol Chang, Sejoon Lee
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Chemosensors
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Online Access:https://www.mdpi.com/2227-9040/13/7/264
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author Sankar Sekar
Ramalingam Manikandan
Shiva Kumar Arumugasamy
Saravanan Sekar
Youngmin Lee
Seung-Cheol Chang
Sejoon Lee
author_facet Sankar Sekar
Ramalingam Manikandan
Shiva Kumar Arumugasamy
Saravanan Sekar
Youngmin Lee
Seung-Cheol Chang
Sejoon Lee
author_sort Sankar Sekar
collection DOAJ
description In this study, a simple and effective approach was developed for the quantitative detection of serotonin. Hexagonal copper selenide nanostructures (CuSe) were employed to modify a disposable screen-printed carbon electrode (SPCE), and their ability to electrochemically detect serotonin in serum samples was investigated. The fabricated CuSe nanostructures exhibited an interconnected, cluster-like morphology composed of irregularly shaped particles with a distinct hexagonal crystal structure. The electrochemical results revealed that the CuSe/SPCE sensor showed better electrochemical activity and good analytical sensing performance towards serotonin detection. The sensor exhibited a linear response in the concentration range of 10 to 1000 nM, with an excellent correlation coefficient (R<sup>2</sup> = 0.9998) and a low detection limit of 3 nM. Furthermore, the CuSe/SPCE showed better selectivity, impressive sensitivity (12.45 µM/µA cm<sup>−2</sup>), and good reproducibility toward serotonin detection, making it a promising electrochemical biosensor for serotonin detection in various real biological samples.
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institution Kabale University
issn 2227-9040
language English
publishDate 2025-07-01
publisher MDPI AG
record_format Article
series Chemosensors
spelling doaj-art-c674e9b1bacb456ca0b6703bbd3ae5822025-08-20T03:58:25ZengMDPI AGChemosensors2227-90402025-07-0113726410.3390/chemosensors13070264Rapid Microwave-Assisted Synthesis of CuSe Nanoparticles for High-Sensitivity Serotonin Biosensing in SerumSankar Sekar0Ramalingam Manikandan1Shiva Kumar Arumugasamy2Saravanan Sekar3Youngmin Lee4Seung-Cheol Chang5Sejoon Lee6Division of System Semiconductor, Dongguk University-Seoul, Seoul 04620, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaDepartment of Chemical and Biochemical Engineering, Dongguk University-Seoul, Seoul 04620, Republic of KoreaDepartment of Mechanical Engineering, K. Ramakrishnan College of Technology, Trichy 621112, IndiaDivision of System Semiconductor, Dongguk University-Seoul, Seoul 04620, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaDivision of System Semiconductor, Dongguk University-Seoul, Seoul 04620, Republic of KoreaIn this study, a simple and effective approach was developed for the quantitative detection of serotonin. Hexagonal copper selenide nanostructures (CuSe) were employed to modify a disposable screen-printed carbon electrode (SPCE), and their ability to electrochemically detect serotonin in serum samples was investigated. The fabricated CuSe nanostructures exhibited an interconnected, cluster-like morphology composed of irregularly shaped particles with a distinct hexagonal crystal structure. The electrochemical results revealed that the CuSe/SPCE sensor showed better electrochemical activity and good analytical sensing performance towards serotonin detection. The sensor exhibited a linear response in the concentration range of 10 to 1000 nM, with an excellent correlation coefficient (R<sup>2</sup> = 0.9998) and a low detection limit of 3 nM. Furthermore, the CuSe/SPCE showed better selectivity, impressive sensitivity (12.45 µM/µA cm<sup>−2</sup>), and good reproducibility toward serotonin detection, making it a promising electrochemical biosensor for serotonin detection in various real biological samples.https://www.mdpi.com/2227-9040/13/7/264CuSe nanoparticlesserotoninbiosensorsynergistic effectserum samples
spellingShingle Sankar Sekar
Ramalingam Manikandan
Shiva Kumar Arumugasamy
Saravanan Sekar
Youngmin Lee
Seung-Cheol Chang
Sejoon Lee
Rapid Microwave-Assisted Synthesis of CuSe Nanoparticles for High-Sensitivity Serotonin Biosensing in Serum
Chemosensors
CuSe nanoparticles
serotonin
biosensor
synergistic effect
serum samples
title Rapid Microwave-Assisted Synthesis of CuSe Nanoparticles for High-Sensitivity Serotonin Biosensing in Serum
title_full Rapid Microwave-Assisted Synthesis of CuSe Nanoparticles for High-Sensitivity Serotonin Biosensing in Serum
title_fullStr Rapid Microwave-Assisted Synthesis of CuSe Nanoparticles for High-Sensitivity Serotonin Biosensing in Serum
title_full_unstemmed Rapid Microwave-Assisted Synthesis of CuSe Nanoparticles for High-Sensitivity Serotonin Biosensing in Serum
title_short Rapid Microwave-Assisted Synthesis of CuSe Nanoparticles for High-Sensitivity Serotonin Biosensing in Serum
title_sort rapid microwave assisted synthesis of cuse nanoparticles for high sensitivity serotonin biosensing in serum
topic CuSe nanoparticles
serotonin
biosensor
synergistic effect
serum samples
url https://www.mdpi.com/2227-9040/13/7/264
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