Laser Emission at 675 nm: Molecular Counteraction of the Aging Process
Background/Objectives: Many lasers applied in skin rejuvenation protocols show emissions with wavelengths falling in the red or near-infrared (NIR) bands. To obtain further in vitro data on the potential therapeutic benefits regarding rejuvenation, we employed a 675 nm laser wavelength on cultured h...
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MDPI AG
2024-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/12/2713 |
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| author | Lorenzo Notari Laura Pieri Francesca Cialdai Irene Fusco Chiara Risaliti Francesca Madeddu Stefano Bacci Tiziano Zingoni Monica Monici |
| author_facet | Lorenzo Notari Laura Pieri Francesca Cialdai Irene Fusco Chiara Risaliti Francesca Madeddu Stefano Bacci Tiziano Zingoni Monica Monici |
| author_sort | Lorenzo Notari |
| collection | DOAJ |
| description | Background/Objectives: Many lasers applied in skin rejuvenation protocols show emissions with wavelengths falling in the red or near-infrared (NIR) bands. To obtain further in vitro data on the potential therapeutic benefits regarding rejuvenation, we employed a 675 nm laser wavelength on cultured human dermal fibroblasts to understand the mechanisms involved in the skin rejuvenation process’s signaling pathways by analyzing cytoskeletal proteins, extracellular matrix (ECM) components, and membrane integrins. Methods: Normal human dermal fibroblasts (NHDFs) were irradiated with a 675 nm laser 24 h after seeding, and immunofluorescence microscopy and Western blotting were applied. Results: The results demonstrate that the laser treatment induces significant changes in human dermal fibroblasts, affecting cytoskeleton organization and the production and reorganization of ECM molecules. The cell response to the treatment appears to predominantly involve paxillin-mediated signaling pathways. Conclusions: These changes suggest that laser treatment can potentially improve the structure and function of skin tissue, with interesting implications for treating skin aging. |
| format | Article |
| id | doaj-art-c65a315cbb5345a7ab3d4fb5a8f954e0 |
| institution | DOAJ |
| issn | 2227-9059 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj-art-c65a315cbb5345a7ab3d4fb5a8f954e02025-08-20T02:53:37ZengMDPI AGBiomedicines2227-90592024-11-011212271310.3390/biomedicines12122713Laser Emission at 675 nm: Molecular Counteraction of the Aging ProcessLorenzo Notari0Laura Pieri1Francesca Cialdai2Irene Fusco3Chiara Risaliti4Francesca Madeddu5Stefano Bacci6Tiziano Zingoni7Monica Monici8ASA Campus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, 50139 Florence, ItalyEl.En. Group, 50041 Calenzano, ItalyASA Campus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, 50139 Florence, ItalyEl.En. Group, 50041 Calenzano, ItalyASA Campus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, 50139 Florence, ItalyEl.En. Group, 50041 Calenzano, ItalyResearch Unit of Histology and Embryology, Department of Biology, University of Florence, 50121 Florence, ItalyEl.En. Group, 50041 Calenzano, ItalyASA Campus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, 50139 Florence, ItalyBackground/Objectives: Many lasers applied in skin rejuvenation protocols show emissions with wavelengths falling in the red or near-infrared (NIR) bands. To obtain further in vitro data on the potential therapeutic benefits regarding rejuvenation, we employed a 675 nm laser wavelength on cultured human dermal fibroblasts to understand the mechanisms involved in the skin rejuvenation process’s signaling pathways by analyzing cytoskeletal proteins, extracellular matrix (ECM) components, and membrane integrins. Methods: Normal human dermal fibroblasts (NHDFs) were irradiated with a 675 nm laser 24 h after seeding, and immunofluorescence microscopy and Western blotting were applied. Results: The results demonstrate that the laser treatment induces significant changes in human dermal fibroblasts, affecting cytoskeleton organization and the production and reorganization of ECM molecules. The cell response to the treatment appears to predominantly involve paxillin-mediated signaling pathways. Conclusions: These changes suggest that laser treatment can potentially improve the structure and function of skin tissue, with interesting implications for treating skin aging.https://www.mdpi.com/2227-9059/12/12/2713675 nm laserextracellular matrix moleculesskin rejuvenation |
| spellingShingle | Lorenzo Notari Laura Pieri Francesca Cialdai Irene Fusco Chiara Risaliti Francesca Madeddu Stefano Bacci Tiziano Zingoni Monica Monici Laser Emission at 675 nm: Molecular Counteraction of the Aging Process Biomedicines 675 nm laser extracellular matrix molecules skin rejuvenation |
| title | Laser Emission at 675 nm: Molecular Counteraction of the Aging Process |
| title_full | Laser Emission at 675 nm: Molecular Counteraction of the Aging Process |
| title_fullStr | Laser Emission at 675 nm: Molecular Counteraction of the Aging Process |
| title_full_unstemmed | Laser Emission at 675 nm: Molecular Counteraction of the Aging Process |
| title_short | Laser Emission at 675 nm: Molecular Counteraction of the Aging Process |
| title_sort | laser emission at 675 nm molecular counteraction of the aging process |
| topic | 675 nm laser extracellular matrix molecules skin rejuvenation |
| url | https://www.mdpi.com/2227-9059/12/12/2713 |
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