The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cells
Zishen Yuzhen Pill (ZYP) is a Chinese herbal product developed by Shenzhen TCM Hospital, which have been frequently used to treat osteoporosis (OP). This study aimed to determine the major chemical components of ZYP and its prototype compounds and metabolites in rat biological samples, as well as ex...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Analytical Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frans.2025.1533486/full |
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| author | Juanjuan Cheng Xinyue Meng Daozheng Fang Yong Zhu Zhihao Liu Xinyue Li Ke Jie Ke Jie Ke Jie Shiying Huang Huilin Li Shangbin Zhang Jihang Chen Jianping Chen |
| author_facet | Juanjuan Cheng Xinyue Meng Daozheng Fang Yong Zhu Zhihao Liu Xinyue Li Ke Jie Ke Jie Ke Jie Shiying Huang Huilin Li Shangbin Zhang Jihang Chen Jianping Chen |
| author_sort | Juanjuan Cheng |
| collection | DOAJ |
| description | Zishen Yuzhen Pill (ZYP) is a Chinese herbal product developed by Shenzhen TCM Hospital, which have been frequently used to treat osteoporosis (OP). This study aimed to determine the major chemical components of ZYP and its prototype compounds and metabolites in rat biological samples, as well as explore the potential effect of ZYP-containing serum in MC3T3-E1 cells. UPLC-Q/TOF-MS was used to identify the chemical components. Then, ZYP was orally administered to rat, and samples of plasma, urine, feces, bile, and tissue were collected to identify prototype compounds and metabolites. The viability of MC3T3-E1 cells was evaluated using the CCK-8 method after treatment with various concentrations (2%, 4%, and 8%) of ZYP-containing serum. Following treatment of MC3T3-E1 cells with ZYP-containing serum, the activity of alkaline phosphatase (ALP) and Alizarin red S (ARS) were measured, and the levels of Runx2, Opn, Opg and Osterix were quantified using the qPCR and Western blot analysis. And cells were collected for RNA-seq analysis. Results indicated that a total 152 compounds were identified in ZYP, including flavonoids, iridoid, lignans, triterpene saponins, etc. Furthermore, we detected a total of 70 prototype components and 99 metabolites distributed in different tissues. In addition, ZYP-containing serum observably promoted osteogenesis by increasing ALP and ARS activities, as well as up-regulating the expression of Runx2, Opn, Opg and Osterix in MC3T3-E1 cells. RNA-seq results indicated that the beneficial effects may be related to the upregulation of mitochondrial oxidative phosphorylation. This work provided further support for the traditional application of ZYP in the treatment of OP. And this study can promote the further pharmacokinetic and pharmacological evaluation of ZYP. |
| format | Article |
| id | doaj-art-c63d2ecff8724352b424ec4302df9932 |
| institution | DOAJ |
| issn | 2673-9283 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Analytical Science |
| spelling | doaj-art-c63d2ecff8724352b424ec4302df99322025-08-20T03:12:53ZengFrontiers Media S.A.Frontiers in Analytical Science2673-92832025-02-01510.3389/frans.2025.15334861533486The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cellsJuanjuan Cheng0Xinyue Meng1Daozheng Fang2Yong Zhu3Zhihao Liu4Xinyue Li5Ke Jie6Ke Jie7Ke Jie8Shiying Huang9Huilin Li10Shangbin Zhang11Jihang Chen12Jianping Chen13Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Medicine, The Chinese University of Hong Kong, Shenzhen, ChinaShenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Medicine, The Chinese University of Hong Kong, Shenzhen, ChinaShenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Medicine, The Chinese University of Hong Kong, Shenzhen, ChinaShenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Medicine, The Chinese University of Hong Kong, Shenzhen, ChinaShenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Medicine, The Chinese University of Hong Kong, Shenzhen, ChinaThe Eighth Clinical Medical College of Guangzhou University of Chinese Medicine, Foshan, ChinaFoshan Hospital of Traditional Chinese Medicine, Foshan, ChinaGuangzhou University of Chinese Medicine, Guangzhou, ChinaShenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Medicine, The Chinese University of Hong Kong, Shenzhen, ChinaShenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaZishen Yuzhen Pill (ZYP) is a Chinese herbal product developed by Shenzhen TCM Hospital, which have been frequently used to treat osteoporosis (OP). This study aimed to determine the major chemical components of ZYP and its prototype compounds and metabolites in rat biological samples, as well as explore the potential effect of ZYP-containing serum in MC3T3-E1 cells. UPLC-Q/TOF-MS was used to identify the chemical components. Then, ZYP was orally administered to rat, and samples of plasma, urine, feces, bile, and tissue were collected to identify prototype compounds and metabolites. The viability of MC3T3-E1 cells was evaluated using the CCK-8 method after treatment with various concentrations (2%, 4%, and 8%) of ZYP-containing serum. Following treatment of MC3T3-E1 cells with ZYP-containing serum, the activity of alkaline phosphatase (ALP) and Alizarin red S (ARS) were measured, and the levels of Runx2, Opn, Opg and Osterix were quantified using the qPCR and Western blot analysis. And cells were collected for RNA-seq analysis. Results indicated that a total 152 compounds were identified in ZYP, including flavonoids, iridoid, lignans, triterpene saponins, etc. Furthermore, we detected a total of 70 prototype components and 99 metabolites distributed in different tissues. In addition, ZYP-containing serum observably promoted osteogenesis by increasing ALP and ARS activities, as well as up-regulating the expression of Runx2, Opn, Opg and Osterix in MC3T3-E1 cells. RNA-seq results indicated that the beneficial effects may be related to the upregulation of mitochondrial oxidative phosphorylation. This work provided further support for the traditional application of ZYP in the treatment of OP. And this study can promote the further pharmacokinetic and pharmacological evaluation of ZYP.https://www.frontiersin.org/articles/10.3389/frans.2025.1533486/fullZishen Yuzhen PillUPLC-QTOF/MSchemical characterizationosteoporosisMC3T3-E1 cells |
| spellingShingle | Juanjuan Cheng Xinyue Meng Daozheng Fang Yong Zhu Zhihao Liu Xinyue Li Ke Jie Ke Jie Ke Jie Shiying Huang Huilin Li Shangbin Zhang Jihang Chen Jianping Chen The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cells Frontiers in Analytical Science Zishen Yuzhen Pill UPLC-QTOF/MS chemical characterization osteoporosis MC3T3-E1 cells |
| title | The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cells |
| title_full | The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cells |
| title_fullStr | The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cells |
| title_full_unstemmed | The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cells |
| title_short | The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cells |
| title_sort | chemicalome profiling of zishen yuzhen pill in vivo and its promoting effect on osteogenic differentiation of mc3t3 e1 cells |
| topic | Zishen Yuzhen Pill UPLC-QTOF/MS chemical characterization osteoporosis MC3T3-E1 cells |
| url | https://www.frontiersin.org/articles/10.3389/frans.2025.1533486/full |
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