pH-responsive magnetic Fe3O4 modified chitosan nanoparticles loaded with β-acids to improve colorectal cancer treatmentStatement of significance
Oral drug delivery systems designed for antitumor drug administration in colorectal cancer treatment encounter substantial challenges regarding effective delivery, controlled release, and intestinal microbiota homeostasis. In this study, dual-stimulation (pH + magnetic) responsive chitosan nanoparti...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-10-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425007215 |
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| author | Songlin Guo Xia Qiao Lu Ding Jiayue Liu Yanan Xu Jia Cao Weidong Tian Duan Ma Xu Zhang Bingren Tian |
| author_facet | Songlin Guo Xia Qiao Lu Ding Jiayue Liu Yanan Xu Jia Cao Weidong Tian Duan Ma Xu Zhang Bingren Tian |
| author_sort | Songlin Guo |
| collection | DOAJ |
| description | Oral drug delivery systems designed for antitumor drug administration in colorectal cancer treatment encounter substantial challenges regarding effective delivery, controlled release, and intestinal microbiota homeostasis. In this study, dual-stimulation (pH + magnetic) responsive chitosan nanoparticles (Fe3O4/chitosan (CS)/TPP) loaded with hops β-acids were synthesized for colorectal cancer treatment. In vitro experiments revealed that the fabricated nanoparticles demonstrated sizes ranging from 233 to 381 nm, with zeta potential values exceeding 10 mV. Release studies indicated that β-acids release was pH-dependent. Notably, the β-acids-loaded nanoparticles proved antimicrobial activity through membrane protein interactions. Cell proliferation assays confirmed that these nanoparticles effectively eliminated HCT116 cells while showing minimal toxicity toward NCM460 cells. Following oral administration to mice with in situ colorectal cancer, histopathological evaluation demonstrated that the nanoparticles induced apoptosis at the tumor site, leading to reduced tumor growth. Additionally, the drug-loaded nanoparticles showed enhanced antitumor efficacy compared to free 5-FU. The findings suggest that these nanoparticles significantly increase beneficial bacterial populations while decreasing levels of harmful bacteria. These dual-function nanoparticles, which exhibit both chemotherapeutic and antimicrobial properties, present a promising novel strategy for treating colorectal cancer. |
| format | Article |
| id | doaj-art-c63ad20fc6cf420db037f5f244e1ef3f |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-c63ad20fc6cf420db037f5f244e1ef3f2025-08-20T03:38:59ZengElsevierMaterials Today Bio2590-00642025-10-013410215110.1016/j.mtbio.2025.102151pH-responsive magnetic Fe3O4 modified chitosan nanoparticles loaded with β-acids to improve colorectal cancer treatmentStatement of significanceSonglin Guo0Xia Qiao1Lu Ding2Jiayue Liu3Yanan Xu4Jia Cao5Weidong Tian6Duan Ma7Xu Zhang8Bingren Tian9Surgery Laboratory, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, ChinaSurgery Laboratory, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, ChinaSurgery Laboratory, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, ChinaSurgery Laboratory, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, ChinaSurgery Laboratory, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, ChinaState Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China; Corresponding author.Surgery Laboratory, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China; Department of Biochemistry and Molecular Biology, Research Center for Birth Defects, Institutes of Biomedical Sciences, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, Shanghai, China; Corresponding author.Surgery Laboratory, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China; Corresponding author.Surgery Laboratory, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China; Corresponding author.Oral drug delivery systems designed for antitumor drug administration in colorectal cancer treatment encounter substantial challenges regarding effective delivery, controlled release, and intestinal microbiota homeostasis. In this study, dual-stimulation (pH + magnetic) responsive chitosan nanoparticles (Fe3O4/chitosan (CS)/TPP) loaded with hops β-acids were synthesized for colorectal cancer treatment. In vitro experiments revealed that the fabricated nanoparticles demonstrated sizes ranging from 233 to 381 nm, with zeta potential values exceeding 10 mV. Release studies indicated that β-acids release was pH-dependent. Notably, the β-acids-loaded nanoparticles proved antimicrobial activity through membrane protein interactions. Cell proliferation assays confirmed that these nanoparticles effectively eliminated HCT116 cells while showing minimal toxicity toward NCM460 cells. Following oral administration to mice with in situ colorectal cancer, histopathological evaluation demonstrated that the nanoparticles induced apoptosis at the tumor site, leading to reduced tumor growth. Additionally, the drug-loaded nanoparticles showed enhanced antitumor efficacy compared to free 5-FU. The findings suggest that these nanoparticles significantly increase beneficial bacterial populations while decreasing levels of harmful bacteria. These dual-function nanoparticles, which exhibit both chemotherapeutic and antimicrobial properties, present a promising novel strategy for treating colorectal cancer.http://www.sciencedirect.com/science/article/pii/S2590006425007215ChitosanHops β-acidsFe3O4pH+magnetic nanoparticleAntibacterialColorectal cancer |
| spellingShingle | Songlin Guo Xia Qiao Lu Ding Jiayue Liu Yanan Xu Jia Cao Weidong Tian Duan Ma Xu Zhang Bingren Tian pH-responsive magnetic Fe3O4 modified chitosan nanoparticles loaded with β-acids to improve colorectal cancer treatmentStatement of significance Materials Today Bio Chitosan Hops β-acids Fe3O4 pH+magnetic nanoparticle Antibacterial Colorectal cancer |
| title | pH-responsive magnetic Fe3O4 modified chitosan nanoparticles loaded with β-acids to improve colorectal cancer treatmentStatement of significance |
| title_full | pH-responsive magnetic Fe3O4 modified chitosan nanoparticles loaded with β-acids to improve colorectal cancer treatmentStatement of significance |
| title_fullStr | pH-responsive magnetic Fe3O4 modified chitosan nanoparticles loaded with β-acids to improve colorectal cancer treatmentStatement of significance |
| title_full_unstemmed | pH-responsive magnetic Fe3O4 modified chitosan nanoparticles loaded with β-acids to improve colorectal cancer treatmentStatement of significance |
| title_short | pH-responsive magnetic Fe3O4 modified chitosan nanoparticles loaded with β-acids to improve colorectal cancer treatmentStatement of significance |
| title_sort | ph responsive magnetic fe3o4 modified chitosan nanoparticles loaded with β acids to improve colorectal cancer treatmentstatement of significance |
| topic | Chitosan Hops β-acids Fe3O4 pH+magnetic nanoparticle Antibacterial Colorectal cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425007215 |
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