Discrimination of Free-Range and Caged Eggs by Chemometrics Analysis of the Elemental Profiles of Eggshell

As one of the foods commonly eaten all over the world, eggs have attracted more and more attention for their quality and price. A method based on elemental profiles and chemometrics to discriminate between free-range and caged eggs was established. Free-range (n1 = 127) and caged (n2 = 122) eggs wer...

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Main Authors: Shunping Xie, Chengying Hai, Song He, Huanhuan Lu, Lu Xu, Haiyan Fu
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2023/1271409
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author Shunping Xie
Chengying Hai
Song He
Huanhuan Lu
Lu Xu
Haiyan Fu
author_facet Shunping Xie
Chengying Hai
Song He
Huanhuan Lu
Lu Xu
Haiyan Fu
author_sort Shunping Xie
collection DOAJ
description As one of the foods commonly eaten all over the world, eggs have attracted more and more attention for their quality and price. A method based on elemental profiles and chemometrics to discriminate between free-range and caged eggs was established. Free-range (n1 = 127) and caged (n2 = 122) eggs were collected from different producing areas in China. The content of 16 elements (Zn, Pb, Cd, Co, Ni, Fe, Mn, Cr, Mg, Cu, Se, Ca, Al, Sr, Na, and K) in the eggshell was determined using a inductively coupled plasma atomic emission spectrometer (ICP-AES). Outlier diagnosis is performed by robust Stahel–Donoho estimation (SDE) and the Kennard and Stone (K-S) algorithm for training and test set partitioning. Partial least squares discriminant analysis (PLS-DA) and least squares support vector machine (LS-SVM) were used for classification of the two types of eggs. As a result, Cd, Mn, Mg, Se, and K make an important contribution to the classification of free-range and caged eggs. By combining column-wise and row-wise rescaling of the elemental data, the sensitivity, specificity, and accuracy were 91.9%, 91.1%, and 92.7% for PLS-DA, while the results of LS-SVM were 95.3%, 95.6%, and 95.1%, respectively. The result indicates that chemometrics analysis of the elemental profiles of eggshells could provide a useful and effective method to discriminate between free-range and caged eggs.
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spelling doaj-art-c62576e62dbd406282df339de32aec062025-08-20T02:18:39ZengWileyJournal of Analytical Methods in Chemistry2090-88732023-01-01202310.1155/2023/1271409Discrimination of Free-Range and Caged Eggs by Chemometrics Analysis of the Elemental Profiles of EggshellShunping Xie0Chengying Hai1Song He2Huanhuan Lu3Lu Xu4Haiyan Fu5Technology CenterThe Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei ProvinceThe Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei ProvinceThe Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei ProvinceCollege of Material and Chemical EngineeringThe Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei ProvinceAs one of the foods commonly eaten all over the world, eggs have attracted more and more attention for their quality and price. A method based on elemental profiles and chemometrics to discriminate between free-range and caged eggs was established. Free-range (n1 = 127) and caged (n2 = 122) eggs were collected from different producing areas in China. The content of 16 elements (Zn, Pb, Cd, Co, Ni, Fe, Mn, Cr, Mg, Cu, Se, Ca, Al, Sr, Na, and K) in the eggshell was determined using a inductively coupled plasma atomic emission spectrometer (ICP-AES). Outlier diagnosis is performed by robust Stahel–Donoho estimation (SDE) and the Kennard and Stone (K-S) algorithm for training and test set partitioning. Partial least squares discriminant analysis (PLS-DA) and least squares support vector machine (LS-SVM) were used for classification of the two types of eggs. As a result, Cd, Mn, Mg, Se, and K make an important contribution to the classification of free-range and caged eggs. By combining column-wise and row-wise rescaling of the elemental data, the sensitivity, specificity, and accuracy were 91.9%, 91.1%, and 92.7% for PLS-DA, while the results of LS-SVM were 95.3%, 95.6%, and 95.1%, respectively. The result indicates that chemometrics analysis of the elemental profiles of eggshells could provide a useful and effective method to discriminate between free-range and caged eggs.http://dx.doi.org/10.1155/2023/1271409
spellingShingle Shunping Xie
Chengying Hai
Song He
Huanhuan Lu
Lu Xu
Haiyan Fu
Discrimination of Free-Range and Caged Eggs by Chemometrics Analysis of the Elemental Profiles of Eggshell
Journal of Analytical Methods in Chemistry
title Discrimination of Free-Range and Caged Eggs by Chemometrics Analysis of the Elemental Profiles of Eggshell
title_full Discrimination of Free-Range and Caged Eggs by Chemometrics Analysis of the Elemental Profiles of Eggshell
title_fullStr Discrimination of Free-Range and Caged Eggs by Chemometrics Analysis of the Elemental Profiles of Eggshell
title_full_unstemmed Discrimination of Free-Range and Caged Eggs by Chemometrics Analysis of the Elemental Profiles of Eggshell
title_short Discrimination of Free-Range and Caged Eggs by Chemometrics Analysis of the Elemental Profiles of Eggshell
title_sort discrimination of free range and caged eggs by chemometrics analysis of the elemental profiles of eggshell
url http://dx.doi.org/10.1155/2023/1271409
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