Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences
The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A...
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Universitas Airlangga, Faculty of Veterinary Medicine
2023-04-01
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| Series: | Jurnal Medik Veteriner |
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| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/40246 |
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| author | Muhammad Fajar Amrullah Budi Utomo Suzanita Utama Tri Wahyu Suprayogi Tita Damayanti Lestari Tjuk Imam Restiadi Ristaqul Husna Belgania |
| author_facet | Muhammad Fajar Amrullah Budi Utomo Suzanita Utama Tri Wahyu Suprayogi Tita Damayanti Lestari Tjuk Imam Restiadi Ristaqul Husna Belgania |
| author_sort | Muhammad Fajar Amrullah |
| collection | DOAJ |
| description | The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds. |
| format | Article |
| id | doaj-art-c619a1ec6dee413ab7ad0d2d1fb14768 |
| institution | DOAJ |
| issn | 2615-7497 2581-012X |
| language | English |
| publishDate | 2023-04-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj-art-c619a1ec6dee413ab7ad0d2d1fb147682025-08-20T02:42:49ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2023-04-016112513110.20473/jmv.vol6.iss1.2023.125-13138317Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA SequencesMuhammad Fajar Amrullah0https://orcid.org/0000-0003-1589-4572Budi Utomo1https://orcid.org/0000-0002-1147-3263Suzanita Utama2https://orcid.org/0000-0003-3907-5969Tri Wahyu Suprayogi3https://orcid.org/0000-0002-2366-5027Tita Damayanti Lestari4https://orcid.org/0000-0001-8561-3452Tjuk Imam Restiadi5https://orcid.org/0000-0001-9382-6546Ristaqul Husna Belgania6https://orcid.org/0000-0003-3668-1760Master of Reproductive Biology, Faculty of Veterinary Medicine, Universitas AirlanggaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas AirlanggaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas AirlanggaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas AirlanggaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas AirlanggaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas AirlanggaMaster of Reproductive Biology, Faculty of Veterinary Medicine, Universitas AirlanggaThe Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.https://e-journal.unair.ac.id/JMV/article/view/40246genetic diversitygoatleptin genephylogenetic treesnps |
| spellingShingle | Muhammad Fajar Amrullah Budi Utomo Suzanita Utama Tri Wahyu Suprayogi Tita Damayanti Lestari Tjuk Imam Restiadi Ristaqul Husna Belgania Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences Jurnal Medik Veteriner genetic diversity goat leptin gene phylogenetic tree snps |
| title | Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences |
| title_full | Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences |
| title_fullStr | Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences |
| title_full_unstemmed | Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences |
| title_short | Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences |
| title_sort | genetic analysis of the leptin gene in goats based on genbank dna sequences |
| topic | genetic diversity goat leptin gene phylogenetic tree snps |
| url | https://e-journal.unair.ac.id/JMV/article/view/40246 |
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