A functional SNP of the core promoter region within goat CDC25A gene affects litter size
The Cell division cycle 25A (CDC25A) gene has been considered as a candidate gene associated with reproductive traits for goat breeding. In this study, five truncated fragments divided at position-2285 nt to +198 nt were amplified and cloned into the luciferase reporter vectors to identify the core...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1471123/full |
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| author | Taiyuan Zhang Jingxuan Wang Yangyang Bai Qian Wang Ke Wang Haijing Zhu Lei Qu Zhengang Guo Chuanying Pan Xianyong Lan |
| author_facet | Taiyuan Zhang Jingxuan Wang Yangyang Bai Qian Wang Ke Wang Haijing Zhu Lei Qu Zhengang Guo Chuanying Pan Xianyong Lan |
| author_sort | Taiyuan Zhang |
| collection | DOAJ |
| description | The Cell division cycle 25A (CDC25A) gene has been considered as a candidate gene associated with reproductive traits for goat breeding. In this study, five truncated fragments divided at position-2285 nt to +198 nt were amplified and cloned into the luciferase reporter vectors to identify the core promoter. The luciferase reporter assay showed that the core promoter of CDC25A was located at position-663 nt to-237 nt. Afterwards, a single nucleotide polymorphism (NC_030829.1:g.51731829A > C) at the core promoter was detected using sequencing and KASP in a population of 1,016 goats and luciferase reporter vectors carrying the A allele or C allele were transfected into cells, respectively. The results displayed that the higher relative luciferase activity was observed in plasmids carrying the A allele rather than the C allele. The litter size of individuals with the AA genotype was significantly better than those with other genotypes, which corresponded to increased transcriptional activity in plasmids carrying the A allele. In short, our study provides a potential molecular genetic marker for improving reproductive efficiency in goat breeding. |
| format | Article |
| id | doaj-art-c614ed1136694aebb21cd7a95ba02dda |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-c614ed1136694aebb21cd7a95ba02dda2025-02-05T07:32:07ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-02-011110.3389/fvets.2024.14711231471123A functional SNP of the core promoter region within goat CDC25A gene affects litter sizeTaiyuan Zhang0Jingxuan Wang1Yangyang Bai2Qian Wang3Ke Wang4Haijing Zhu5Lei Qu6Zhengang Guo7Chuanying Pan8Xianyong Lan9College of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, College of Life Science, Yulin University, Yulin, ChinaShaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, College of Life Science, Yulin University, Yulin, ChinaTesting Center for Livestock and Poultry Germplasm, Guiyang, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaThe Cell division cycle 25A (CDC25A) gene has been considered as a candidate gene associated with reproductive traits for goat breeding. In this study, five truncated fragments divided at position-2285 nt to +198 nt were amplified and cloned into the luciferase reporter vectors to identify the core promoter. The luciferase reporter assay showed that the core promoter of CDC25A was located at position-663 nt to-237 nt. Afterwards, a single nucleotide polymorphism (NC_030829.1:g.51731829A > C) at the core promoter was detected using sequencing and KASP in a population of 1,016 goats and luciferase reporter vectors carrying the A allele or C allele were transfected into cells, respectively. The results displayed that the higher relative luciferase activity was observed in plasmids carrying the A allele rather than the C allele. The litter size of individuals with the AA genotype was significantly better than those with other genotypes, which corresponded to increased transcriptional activity in plasmids carrying the A allele. In short, our study provides a potential molecular genetic marker for improving reproductive efficiency in goat breeding.https://www.frontiersin.org/articles/10.3389/fvets.2024.1471123/fullgoatlitter sizeCDC25A genepromoter activitySNPKASP |
| spellingShingle | Taiyuan Zhang Jingxuan Wang Yangyang Bai Qian Wang Ke Wang Haijing Zhu Lei Qu Zhengang Guo Chuanying Pan Xianyong Lan A functional SNP of the core promoter region within goat CDC25A gene affects litter size Frontiers in Veterinary Science goat litter size CDC25A gene promoter activity SNP KASP |
| title | A functional SNP of the core promoter region within goat CDC25A gene affects litter size |
| title_full | A functional SNP of the core promoter region within goat CDC25A gene affects litter size |
| title_fullStr | A functional SNP of the core promoter region within goat CDC25A gene affects litter size |
| title_full_unstemmed | A functional SNP of the core promoter region within goat CDC25A gene affects litter size |
| title_short | A functional SNP of the core promoter region within goat CDC25A gene affects litter size |
| title_sort | functional snp of the core promoter region within goat cdc25a gene affects litter size |
| topic | goat litter size CDC25A gene promoter activity SNP KASP |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1471123/full |
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