Icariin-loaded composite scaffold promotes osteogenic differentiation and bone regeneration
Abstract Background Bone defects remain a significant challenge in orthopedics, and traditional treatments often face limitations. Icariin (ICA) has been shown to promote osteogenic differentiation and angiogenesis, which may benefit bone repair. Methods ICA-loaded microspheres were prepared using a...
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BMC
2025-06-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | https://doi.org/10.1186/s12891-025-08824-4 |
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| author | Dong-Sheng Jin Zhang-Hong Zhao Shi-Qiang Ruan Wen-Liang Huang Ren-Yuan Tian Yu Wan Jiang Deng |
| author_facet | Dong-Sheng Jin Zhang-Hong Zhao Shi-Qiang Ruan Wen-Liang Huang Ren-Yuan Tian Yu Wan Jiang Deng |
| author_sort | Dong-Sheng Jin |
| collection | DOAJ |
| description | Abstract Background Bone defects remain a significant challenge in orthopedics, and traditional treatments often face limitations. Icariin (ICA) has been shown to promote osteogenic differentiation and angiogenesis, which may benefit bone repair. Methods ICA-loaded microspheres were prepared using an evaporation method with a co-solvent system. The encapsulation efficiency, drug loading, and release characteristics were evaluated. Silk fibroin/chitosan/nano-hydroxyapatite (SF/CS/nHA) composite scaffolds incorporated with ICA microspheres were fabricated using vacuum freeze-drying. Bone marrow mesenchymal stem cells (BMSCs) were cultured on these scaffolds in vitro. Scanning electron microscopy (SEM) was used to observe the morphology of microspheres and scaffolds, as well as cell adhesion. In vitro assessments of BMSC morphology, proliferation, and migration on different scaffolds were conducted using CCK-8 assays, live/dead staining, and scratch tests. Osteogenic differentiation was evaluated by alkaline phosphatase staining, Alizarin Red staining, immunofluorescence, RT-qPCR, and Western blotting. A rabbit radial critical-size bone defect model was established in vivo, and SF/CS/nHA-ICA composite scaffolds were implanted at the defect sites. Bone repair effects were assessed by CT imaging, hematoxylin-eosin (H&E) staining, and Masson’s trichrome staining. Osteogenic and angiogenic protein expression levels were further analyzed by immunohistochemistry and Western blot. Results In vitro experiments demonstrated that the SF/CS/nHA-ICA group had superior BMSC adhesion, cell morphology, proliferation, and osteogenic differentiation compared to other groups (P < 0.05). In vivo, evaluations indicated that the addition of ICA significantly enhanced bone regeneration and vascularization at the defect sites compared to control and other experimental groups. Western blot and immunohistochemical analyses confirmed significant upregulation of osteogenic and angiogenic proteins (type I collagen, runt-related transcription factor 2, osteocalcin, vascular endothelial growth factor) in the SF/CS/nHA-BMSCs-ICA group. Conclusion ICA-loaded scaffolds effectively promote bone regeneration and repair of bone defects, offering a potential strategy for the treatment of bone defects. |
| format | Article |
| id | doaj-art-c60327ed3ad94c3c9cbf4a17fd74c8cc |
| institution | OA Journals |
| issn | 1471-2474 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
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| series | BMC Musculoskeletal Disorders |
| spelling | doaj-art-c60327ed3ad94c3c9cbf4a17fd74c8cc2025-08-20T02:05:43ZengBMCBMC Musculoskeletal Disorders1471-24742025-06-0126112010.1186/s12891-025-08824-4Icariin-loaded composite scaffold promotes osteogenic differentiation and bone regenerationDong-Sheng Jin0Zhang-Hong Zhao1Shi-Qiang Ruan2Wen-Liang Huang3Ren-Yuan Tian4Yu Wan5Jiang Deng6Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi First People’s HospitalDepartment of Orthopaedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi First People’s HospitalDepartment of Orthopaedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi First People’s HospitalDepartment of Orthopaedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi First People’s HospitalDepartment of Orthopaedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi First People’s HospitalDepartment of Orthopaedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi First People’s HospitalDepartment of Orthopaedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi First People’s HospitalAbstract Background Bone defects remain a significant challenge in orthopedics, and traditional treatments often face limitations. Icariin (ICA) has been shown to promote osteogenic differentiation and angiogenesis, which may benefit bone repair. Methods ICA-loaded microspheres were prepared using an evaporation method with a co-solvent system. The encapsulation efficiency, drug loading, and release characteristics were evaluated. Silk fibroin/chitosan/nano-hydroxyapatite (SF/CS/nHA) composite scaffolds incorporated with ICA microspheres were fabricated using vacuum freeze-drying. Bone marrow mesenchymal stem cells (BMSCs) were cultured on these scaffolds in vitro. Scanning electron microscopy (SEM) was used to observe the morphology of microspheres and scaffolds, as well as cell adhesion. In vitro assessments of BMSC morphology, proliferation, and migration on different scaffolds were conducted using CCK-8 assays, live/dead staining, and scratch tests. Osteogenic differentiation was evaluated by alkaline phosphatase staining, Alizarin Red staining, immunofluorescence, RT-qPCR, and Western blotting. A rabbit radial critical-size bone defect model was established in vivo, and SF/CS/nHA-ICA composite scaffolds were implanted at the defect sites. Bone repair effects were assessed by CT imaging, hematoxylin-eosin (H&E) staining, and Masson’s trichrome staining. Osteogenic and angiogenic protein expression levels were further analyzed by immunohistochemistry and Western blot. Results In vitro experiments demonstrated that the SF/CS/nHA-ICA group had superior BMSC adhesion, cell morphology, proliferation, and osteogenic differentiation compared to other groups (P < 0.05). In vivo, evaluations indicated that the addition of ICA significantly enhanced bone regeneration and vascularization at the defect sites compared to control and other experimental groups. Western blot and immunohistochemical analyses confirmed significant upregulation of osteogenic and angiogenic proteins (type I collagen, runt-related transcription factor 2, osteocalcin, vascular endothelial growth factor) in the SF/CS/nHA-BMSCs-ICA group. Conclusion ICA-loaded scaffolds effectively promote bone regeneration and repair of bone defects, offering a potential strategy for the treatment of bone defects.https://doi.org/10.1186/s12891-025-08824-4IcariinBiomimetic bone scaffoldBone marrow mesenchymal stem cellsBone regenerationBone tissue engineering |
| spellingShingle | Dong-Sheng Jin Zhang-Hong Zhao Shi-Qiang Ruan Wen-Liang Huang Ren-Yuan Tian Yu Wan Jiang Deng Icariin-loaded composite scaffold promotes osteogenic differentiation and bone regeneration BMC Musculoskeletal Disorders Icariin Biomimetic bone scaffold Bone marrow mesenchymal stem cells Bone regeneration Bone tissue engineering |
| title | Icariin-loaded composite scaffold promotes osteogenic differentiation and bone regeneration |
| title_full | Icariin-loaded composite scaffold promotes osteogenic differentiation and bone regeneration |
| title_fullStr | Icariin-loaded composite scaffold promotes osteogenic differentiation and bone regeneration |
| title_full_unstemmed | Icariin-loaded composite scaffold promotes osteogenic differentiation and bone regeneration |
| title_short | Icariin-loaded composite scaffold promotes osteogenic differentiation and bone regeneration |
| title_sort | icariin loaded composite scaffold promotes osteogenic differentiation and bone regeneration |
| topic | Icariin Biomimetic bone scaffold Bone marrow mesenchymal stem cells Bone regeneration Bone tissue engineering |
| url | https://doi.org/10.1186/s12891-025-08824-4 |
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